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The map label for this gene is ada

Identifier: 15836128

GI number: 15836128

Start: 685260

End: 685781

Strand: Direct

Name: ada

Synonym: CPj0596

Alternate gene names: 15836128

Gene position: 685260-685781 (Clockwise)

Preceding gene: 15836127

Following gene: 15836135

Centisome position: 55.87

GC content: 43.87

Gene sequence:

>522_bases
ATGGCTGATGACACCCTCATTCCTAAACTTATGAAGAACTCGCTTTCGCAGGCGTGTTCTGAGGGTTTACTGATTGCTAA
GTATCCTCCACTCCAGGTTATCGTTCACTTTGATAATAACCTAGTTGTTAAAACACATCTTTCAGTAGCTCCTGTCTTCT
CTTGTCTTTTTTTAGGACCAGCAGCTCACAAAGCCATGCAGGAAATTGTTTTATGGTGTTCTCGCTATGCCAACAAGGAA
CATCCTCCTTTTTCCTCGCATTTTGCTAAAGACCTCATCCCCTCACAATATCTCGAAATCCTAAACTGCGTTGCAGAGAT
TCCCTTTGGAGAGCAGCAAACCTACGCTGAAATCGCAAAAAAAACTGATACGCACCCCAGGACTGTAGGAGCCGCATGCA
AACAAAATCCGTTTCTGCTGTTCTTTCCCTGTCATCGCGTCGTAGGAAGCCATGGAGAGCGTAATTACGTCCTAGGGCCT
GTAATTCACGAGATCTTATTGAAATTTGAGAATAGCTACTAA

Upstream 100 bases:

>100_bases
CTATGCTGGAGGAATCCAAAAGCATGAATGGTATTACCGCGGGAGATCTGTATCTAAAGCCAAATTCGAGCGGCTAAATG
CTGCAGGATAGTTTGCGGTA

Downstream 100 bases:

>100_bases
TCCCCAGCTATAGATTTAAGAGTCTTGCAGACGGGGATCTAAAGCATCACGGACTCCGTCTCCTATCAGAGCGATCGCAA
TCAGCAACATCGTTAATATA

Product: methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MADDTLIPKLMKNSLSQACSEGLLIAKYPPLQVIVHFDNNLVVKTHLSVAPVFSCLFLGPAAHKAMQEIVLWCSRYANKE
HPPFSSHFAKDLIPSQYLEILNCVAEIPFGEQQTYAEIAKKTDTHPRTVGAACKQNPFLLFFPCHRVVGSHGERNYVLGP
VIHEILLKFENSY

Sequences:

>Translated_173_residues
MADDTLIPKLMKNSLSQACSEGLLIAKYPPLQVIVHFDNNLVVKTHLSVAPVFSCLFLGPAAHKAMQEIVLWCSRYANKE
HPPFSSHFAKDLIPSQYLEILNCVAEIPFGEQQTYAEIAKKTDTHPRTVGAACKQNPFLLFFPCHRVVGSHGERNYVLGP
VIHEILLKFENSY
>Mature_172_residues
ADDTLIPKLMKNSLSQACSEGLLIAKYPPLQVIVHFDNNLVVKTHLSVAPVFSCLFLGPAAHKAMQEIVLWCSRYANKEH
PPFSSHFAKDLIPSQYLEILNCVAEIPFGEQQTYAEIAKKTDTHPRTVGAACKQNPFLLFFPCHRVVGSHGERNYVLGPV
IHEILLKFENSY

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19405; Mature: 19274

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDTLIPKLMKNSLSQACSEGLLIAKYPPLQVIVHFDNNLVVKTHLSVAPVFSCLFLGP
CCCCHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCEEEEECCHHHHHHHHHHHCH
AAHKAMQEIVLWCSRYANKEHPPFSSHFAKDLIPSQYLEILNCVAEIPFGEQQTYAEIAK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
KTDTHPRTVGAACKQNPFLLFFPCHRVVGSHGERNYVLGPVIHEILLKFENSY
HCCCCCHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADDTLIPKLMKNSLSQACSEGLLIAKYPPLQVIVHFDNNLVVKTHLSVAPVFSCLFLGP
CCCHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCEEEEECCHHHHHHHHHHHCH
AAHKAMQEIVLWCSRYANKEHPPFSSHFAKDLIPSQYLEILNCVAEIPFGEQQTYAEIAK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
KTDTHPRTVGAACKQNPFLLFFPCHRVVGSHGERNYVLGPVIHEILLKFENSY
HCCCCCHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]