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The map label for this gene is gltX

Identifier: 15836091

GI number: 15836091

Start: 643416

End: 644933

Strand: Reverse

Name: gltX

Synonym: CPj0560

Alternate gene names: 15836091

Gene position: 644933-643416 (Counterclockwise)

Preceding gene: 15836092

Following gene: 15836089

Centisome position: 52.58

GC content: 45.52

Gene sequence:

>1518_bases
ATGAATTGGGAAAATGTCCGTGTTAGGGTAGCTCCTTCACCTACAGGAGATCCTCATGTAGGTACCGCCTATATGGCTCT
GTTTAATGAAATCTTTGCAAAACGATTTAAAGGGAAAATGATCCTCCGTATCGAAGATACGGATAGAACACGTAGTCGCC
AAGATTACGAAGAAAATATTTTCTCAGCTCTTCGTTGGTGCGGGATCCAATGGGATGAGGGCCCTGATGTAGGCGGCCCC
TATGGCCCCTATCGTCAGTCAGAACGCACGAAAATCTATCAAGGGTATGTTGAGACACTTTTAAAGACAGATTGCGCTTA
TAAGTGCTTTGCAACCCCTCAAGAACTCGCAGAAATGCGCGCTGTGGCAAGTACCCTCGGTTATCGTGGGGGATACGATC
GCAGGTACAGGTACCTCTCTCCCGAAGAGGTAGCCTCACGAGAGGCAGCAGGTCAGCCCTACACCATTCGTCTTAAAGTT
CCCTTATCAGGGGAATGTGTTTTCGAAGATTATAGCAAGGGAAGAGTCGTGTTTCCTTGGGCAGATGTCGATGATCAGGT
CCTAGTAAAATCTGACGGATTCCCCACGTACCACTTTGCTAATGTAATTGACGACCACCTCATGGGCATCACTCACGTCC
TCAGAGGGGAAGAGTGGCTAAGTTCAACTCCTAAGCATCTCCTACTCTACGAAGCTTTTGGCTGGGAGCCTCCCGTCTTT
CTCCATATGCCCCTGCTTCTAAACCCCGATGGAACAAAACTTTCAAAAAGAAAGAACCCTACCTCGATTTTTTATTACCG
CGACTCAGGTTATGTCAAAGAAGCCTTTGTGAACTTCCTGACTCTCATGGGTTACAGTATGGAAGGGGATGAAGAAGTCT
ATTCTCTAGAGCGTATTATAGAAACTTTTAATCCTCGACGTATTGGAAAGTCAGGAGCAGTTTTTGATATTCAAAAGCTC
GACTGGATGAATAAGCATTACCTCAACCACGAGGGTTCGCCAGAGTGCTTGTTAAAAGAACTCCAGGGTTGGCTACTTAA
TGACGAATTTTTCTTAAAAATCCTTCCCCTCTGTCAATCTCGGATTACAACTCTTGCGGAGTTTATCAACCTCACCTCGT
TCTTTTTCTCAGGATTGTTAGAGTACCGTGTTGAGGAACTCCTACCACAGGCCCTTTCTCCAGAGAAAGCAGCTATCCTT
CTCTATAGCTATGTCAAATATCTTGAGAAAACCGATCAATGGACGAAAGAGACGTGTTACCTCGGATCTAAATGGTTAGC
TCAAGCATTCAATGTCCACCATAAAAAAGCCATCATCCCACTGCTTTATGTGGCAATTACTGGGAAAAAGCAGGGGCTCC
CTCTCTTTGACTCTATAGAGATTTTAGGGAAACCTCGAGCGAGAGCACGTCTTGTGTATGCAGAAAAGCTTTTAGGAGGC
GTTCCTAAGAAGCTCGCTGCAACTGTGGATAAGTTCATGCAACGAGAAGACTTCGAAGAGGCTACTTTCGATCTCTAA

Upstream 100 bases:

>100_bases
TTTTATTTTTGGATTTATGCATTTCTGCAGAGAGGATTTCCCTTGAAGAAATAGCCGTTTTCAGGGTATGAAGTCTCTCT
GGTCTAAGGATAAAAGAATC

Downstream 100 bases:

>100_bases
ATATCGGTCGCATGAATGTGCGTGGCTGCTAGCTAAGCGAAACAGCCACCGCGGTTTCTATTCATAACCACACTTCCTGT
TTGAGTCGTTGCGAAGACAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNWENVRVRVAPSPTGDPHVGTAYMALFNEIFAKRFKGKMILRIEDTDRTRSRQDYEENIFSALRWCGIQWDEGPDVGGP
YGPYRQSERTKIYQGYVETLLKTDCAYKCFATPQELAEMRAVASTLGYRGGYDRRYRYLSPEEVASREAAGQPYTIRLKV
PLSGECVFEDYSKGRVVFPWADVDDQVLVKSDGFPTYHFANVIDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPVF
LHMPLLLNPDGTKLSKRKNPTSIFYYRDSGYVKEAFVNFLTLMGYSMEGDEEVYSLERIIETFNPRRIGKSGAVFDIQKL
DWMNKHYLNHEGSPECLLKELQGWLLNDEFFLKILPLCQSRITTLAEFINLTSFFFSGLLEYRVEELLPQALSPEKAAIL
LYSYVKYLEKTDQWTKETCYLGSKWLAQAFNVHHKKAIIPLLYVAITGKKQGLPLFDSIEILGKPRARARLVYAEKLLGG
VPKKLAATVDKFMQREDFEEATFDL

Sequences:

>Translated_505_residues
MNWENVRVRVAPSPTGDPHVGTAYMALFNEIFAKRFKGKMILRIEDTDRTRSRQDYEENIFSALRWCGIQWDEGPDVGGP
YGPYRQSERTKIYQGYVETLLKTDCAYKCFATPQELAEMRAVASTLGYRGGYDRRYRYLSPEEVASREAAGQPYTIRLKV
PLSGECVFEDYSKGRVVFPWADVDDQVLVKSDGFPTYHFANVIDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPVF
LHMPLLLNPDGTKLSKRKNPTSIFYYRDSGYVKEAFVNFLTLMGYSMEGDEEVYSLERIIETFNPRRIGKSGAVFDIQKL
DWMNKHYLNHEGSPECLLKELQGWLLNDEFFLKILPLCQSRITTLAEFINLTSFFFSGLLEYRVEELLPQALSPEKAAIL
LYSYVKYLEKTDQWTKETCYLGSKWLAQAFNVHHKKAIIPLLYVAITGKKQGLPLFDSIEILGKPRARARLVYAEKLLGG
VPKKLAATVDKFMQREDFEEATFDL
>Mature_505_residues
MNWENVRVRVAPSPTGDPHVGTAYMALFNEIFAKRFKGKMILRIEDTDRTRSRQDYEENIFSALRWCGIQWDEGPDVGGP
YGPYRQSERTKIYQGYVETLLKTDCAYKCFATPQELAEMRAVASTLGYRGGYDRRYRYLSPEEVASREAAGQPYTIRLKV
PLSGECVFEDYSKGRVVFPWADVDDQVLVKSDGFPTYHFANVIDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPVF
LHMPLLLNPDGTKLSKRKNPTSIFYYRDSGYVKEAFVNFLTLMGYSMEGDEEVYSLERIIETFNPRRIGKSGAVFDIQKL
DWMNKHYLNHEGSPECLLKELQGWLLNDEFFLKILPLCQSRITTLAEFINLTSFFFSGLLEYRVEELLPQALSPEKAAIL
LYSYVKYLEKTDQWTKETCYLGSKWLAQAFNVHHKKAIIPLLYVAITGKKQGLPLFDSIEILGKPRARARLVYAEKLLGG
VPKKLAATVDKFMQREDFEEATFDL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=37.1428571428571, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI62241042, Length=257, Percent_Identity=27.2373540856031, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=33.2640332640333, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI226510925, Length=296, Percent_Identity=30.0675675675676, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=33.4710743801653, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=36.6396761133603, Blast_Score=274, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24665321, Length=501, Percent_Identity=37.7245508982036, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24649466, Length=271, Percent_Identity=27.3062730627306, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHLPN (Q9Z7Z3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C72064
- PIR:   D86560
- RefSeq:   NP_224756.1
- RefSeq:   NP_300615.1
- RefSeq:   NP_444741.1
- RefSeq:   NP_876854.1
- ProteinModelPortal:   Q9Z7Z3
- SMR:   Q9Z7Z3
- GeneID:   1467261
- GeneID:   895358
- GeneID:   919322
- GeneID:   962974
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0190
- KEGG:   cpn:CPn0560
- KEGG:   cpt:CpB0582
- TIGR:   CP_0190
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q9Z7Z3
- ProtClustDB:   PRK01406
- BioCyc:   CPNE115711:CP_0190-MONOMER
- BioCyc:   CPNE115713:CPN0560-MONOMER
- BioCyc:   CPNE138677:CPJ0560-MONOMER
- BioCyc:   CPNE182082:CPB0582-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58206; Mature: 58206

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWENVRVRVAPSPTGDPHVGTAYMALFNEIFAKRFKGKMILRIEDTDRTRSRQDYEENI
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FSALRWCGIQWDEGPDVGGPYGPYRQSERTKIYQGYVETLLKTDCAYKCFATPQELAEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
AVASTLGYRGGYDRRYRYLSPEEVASREAAGQPYTIRLKVPLSGECVFEDYSKGRVVFPW
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCEEEEEE
ADVDDQVLVKSDGFPTYHFANVIDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPVF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCEE
LHMPLLLNPDGTKLSKRKNPTSIFYYRDSGYVKEAFVNFLTLMGYSMEGDEEVYSLERII
EEECEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ETFNPRRIGKSGAVFDIQKLDWMNKHYLNHEGSPECLLKELQGWLLNDEFFLKILPLCQS
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RITTLAEFINLTSFFFSGLLEYRVEELLPQALSPEKAAILLYSYVKYLEKTDQWTKETCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGSKWLAQAFNVHHKKAIIPLLYVAITGKKQGLPLFDSIEILGKPRARARLVYAEKLLGG
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
VPKKLAATVDKFMQREDFEEATFDL
CCHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MNWENVRVRVAPSPTGDPHVGTAYMALFNEIFAKRFKGKMILRIEDTDRTRSRQDYEENI
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FSALRWCGIQWDEGPDVGGPYGPYRQSERTKIYQGYVETLLKTDCAYKCFATPQELAEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
AVASTLGYRGGYDRRYRYLSPEEVASREAAGQPYTIRLKVPLSGECVFEDYSKGRVVFPW
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCEEEEEE
ADVDDQVLVKSDGFPTYHFANVIDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPVF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCEE
LHMPLLLNPDGTKLSKRKNPTSIFYYRDSGYVKEAFVNFLTLMGYSMEGDEEVYSLERII
EEECEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ETFNPRRIGKSGAVFDIQKLDWMNKHYLNHEGSPECLLKELQGWLLNDEFFLKILPLCQS
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RITTLAEFINLTSFFFSGLLEYRVEELLPQALSPEKAAILLYSYVKYLEKTDQWTKETCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGSKWLAQAFNVHHKKAIIPLLYVAITGKKQGLPLFDSIEILGKPRARARLVYAEKLLGG
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
VPKKLAATVDKFMQREDFEEATFDL
CCHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362