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Identifier: 15836062

GI number: 15836062

Start: 612562

End: 613389

Strand: Reverse

Name: Not Available

Synonym: CPj0531

Alternate gene names: 15836062

Gene position: 613389-612562 (Counterclockwise)

Preceding gene: 15836063

Following gene: 15836061

Centisome position: 50.01

GC content: 43.24

Gene sequence:

>828_bases
ATGGATTATAAATTGCTCGATAGTGGGGACGGGAACAAATTAGAGTGTTTTGGTCCTGTCACTTTAATTCGTCCTTCAAG
TATTGCTGTTTGGCCAAAAAGCAGACCTGAACTATGGTCTCAGGCGCAATTGCAGTATGTTCGTGAAGGAGAAAGAGGAG
CATGGAAAAATTTTAAGCGTCTTCCTGAAGAATGGGAGGTCGCATTTTCTGATGTGCGTTGTCTATTGAAGAGAACCCCC
TTTGGGCATCTAGGCGTATTTCCTGAACATATGGGGTTCTGGCCCGCTTTGAAACAAGCCATTGAGAAGCACAAAGAACG
TCAGGTATTGAATCTCTTTGCTTATACAGGGGCGGGTTCTATTTTTGCAGCTAAGTGTGGAGCTCGTGTGACCCATGTAG
ATGCTTCGCAGGCAGCGGTACGTTGGGCGCAAAGAAATGTAGAGAAAAATGCTTTTCCTGAGAGACGTATTTTTTGGGTT
ATTGAAGATGTGATTTCTTTTTTAAAAAAAGAAATCCGCAGAAATAAGAAATATCAAGTGATTCTTTTAGATCCCCCAAG
CTATGGTCGCGGACCTGATGGGGAAGTATTTAAAATAGATAAAGATCTCTTTCCTTTGCTTTCTTTGTGTTCGAAGCTTC
TTGCAGATGACGCTTCTTATTTTCTCTTAACCTCTCATACTCCAGGACATACCCCAGAGTTTTTAAGGGCTATAGCTAGG
CGTAGCGTGCCAACCCTTGTTTCTGAAGCGTGGTCTTGTGGGGAAAGTTTTTGTGGAGAAGGTGTGGGAGCCTTGCCTTC
TGGGAGTTTTGTTCAATGGATTGCATAG

Upstream 100 bases:

>100_bases
AAAGGGAAGGAGAAAGAGTCAATATCGAGATCGATATGTCAACAAAGATACAGGTAGACACAGTAAAACGTATTTTAGCC
TCATCAGGGAAAGATTAGTT

Downstream 100 bases:

>100_bases
GGAAACATAATCCTCTAGTTAAAGAGGCTCTAGCTTTAAAACGGTCTCGTTGTAGAAAAAGTTCTTGGTTTCTAGTTGAA
GGAGCCCGTGAGATTCAGAA

Product: SAM dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDYKLLDSGDGNKLECFGPVTLIRPSSIAVWPKSRPELWSQAQLQYVREGERGAWKNFKRLPEEWEVAFSDVRCLLKRTP
FGHLGVFPEHMGFWPALKQAIEKHKERQVLNLFAYTGAGSIFAAKCGARVTHVDASQAAVRWAQRNVEKNAFPERRIFWV
IEDVISFLKKEIRRNKKYQVILLDPPSYGRGPDGEVFKIDKDLFPLLSLCSKLLADDASYFLLTSHTPGHTPEFLRAIAR
RSVPTLVSEAWSCGESFCGEGVGALPSGSFVQWIA

Sequences:

>Translated_275_residues
MDYKLLDSGDGNKLECFGPVTLIRPSSIAVWPKSRPELWSQAQLQYVREGERGAWKNFKRLPEEWEVAFSDVRCLLKRTP
FGHLGVFPEHMGFWPALKQAIEKHKERQVLNLFAYTGAGSIFAAKCGARVTHVDASQAAVRWAQRNVEKNAFPERRIFWV
IEDVISFLKKEIRRNKKYQVILLDPPSYGRGPDGEVFKIDKDLFPLLSLCSKLLADDASYFLLTSHTPGHTPEFLRAIAR
RSVPTLVSEAWSCGESFCGEGVGALPSGSFVQWIA
>Mature_275_residues
MDYKLLDSGDGNKLECFGPVTLIRPSSIAVWPKSRPELWSQAQLQYVREGERGAWKNFKRLPEEWEVAFSDVRCLLKRTP
FGHLGVFPEHMGFWPALKQAIEKHKERQVLNLFAYTGAGSIFAAKCGARVTHVDASQAAVRWAQRNVEKNAFPERRIFWV
IEDVISFLKKEIRRNKKYQVILLDPPSYGRGPDGEVFKIDKDLFPLLSLCSKLLADDASYFLLTSHTPGHTPEFLRAIAR
RSVPTLVSEAWSCGESFCGEGVGALPSGSFVQWIA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=194, Percent_Identity=26.2886597938144, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 31100; Mature: 31100

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKLLDSGDGNKLECFGPVTLIRPSSIAVWPKSRPELWSQAQLQYVREGERGAWKNFKR
CCCEECCCCCCCEEEEECCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LPEEWEVAFSDVRCLLKRTPFGHLGVFPEHMGFWPALKQAIEKHKERQVLNLFAYTGAGS
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
IFAAKCGARVTHVDASQAAVRWAQRNVEKNAFPERRIFWVIEDVISFLKKEIRRNKKYQV
EEHHHCCCEEEECCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ILLDPPSYGRGPDGEVFKIDKDLFPLLSLCSKLLADDASYFLLTSHTPGHTPEFLRAIAR
EEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RSVPTLVSEAWSCGESFCGEGVGALPSGSFVQWIA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MDYKLLDSGDGNKLECFGPVTLIRPSSIAVWPKSRPELWSQAQLQYVREGERGAWKNFKR
CCCEECCCCCCCEEEEECCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LPEEWEVAFSDVRCLLKRTPFGHLGVFPEHMGFWPALKQAIEKHKERQVLNLFAYTGAGS
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
IFAAKCGARVTHVDASQAAVRWAQRNVEKNAFPERRIFWVIEDVISFLKKEIRRNKKYQV
EEHHHCCCEEEECCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ILLDPPSYGRGPDGEVFKIDKDLFPLLSLCSKLLADDASYFLLTSHTPGHTPEFLRAIAR
EEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RSVPTLVSEAWSCGESFCGEGVGALPSGSFVQWIA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA