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The map label for this gene is glyA

Identifier: 15836052

GI number: 15836052

Start: 601558

End: 603117

Strand: Reverse

Name: glyA

Synonym: CPj0521

Alternate gene names: 15836052

Gene position: 603117-601558 (Counterclockwise)

Preceding gene: 15836057

Following gene: 15836051

Centisome position: 49.17

GC content: 41.67

Gene sequence:

>1560_bases
TTGCTAAAAGTTTTTGAGAAATTTAAGAAATTCGCAATAGTGGAAATATTTACAAAGGTGGTTGCGGTGGTTTCGTTGTT
GCATAAGTTTTTAGAAAATGCTTCGGGGAAAAAGGGACAAAGTTTAGCTTCGACAGCGTATTTAGCAGCTCTTGACCATC
TCTTAAATGCGTTTCCTTCCATTGGGGAGAGAATCATTGATGAGTTGAAGAGCCAGCGTTCCCATTTAAAGATGATTGCT
TCTGAAAACTATTCTTCACTTTCAGTGCAGTTGGCTATGGGGAACTTGCTCACAGATAAGTATTGTGAAGGAAGTCCCTT
TAAGCGTTTCTATTCCTGTTGTGAAAATGTAGATGCTATTGAGTGGGAGTGTGTAGAGACAGCGAAAGAACTTTTTGCTG
CGGATTGCGCTTGTGTTCAGCCTCATTCTGGGGCTGATGCTAATTTACTGGCAGTAATGGCCATTCTCACGCACAAAGTC
CAAGGCCCAGCTGTCAGTAAGTTAGGTTATAAAACTGTAAACGAATTAACAGAAGAAGAATACACTCTACTTAAGGCTGA
AATGTCTTCTTGTGTTTGCTTAGGACCTTCATTAAATTCTGGAGGCCATTTGACCCATGGGAACGTACGTTTAAATGTGA
TGTCTAAGCTTATGCGTTGCTTCCCCTATGATGTCAATCCGGATACGGAGTGTTTTGATTATGCAGAGATCTCCCGGTTA
GCTAAGGAGTATAAACCTAAGGTACTGATCGCAGGATATTCTTCCTATTCTCGAAGATTAAACTTTGCAGTTTTAAAACA
GATTGCAGAGGATTGTGGATCTGTCTTGTGGGTAGATATGGCGCATTTTGCAGGCCTAGTTGCTGGGGGAGTGTTTGTTG
ATGAAGAAAATCCTATTCCTTATGCAGATATAGTGACAACAACAACGCATAAGACATTACGCGGTCCTCGCGGGGGATTA
GTTTTGGCAACTCGAGAGTATGAAAGCACTCTCAATAAGGCGTGTCCTTTGATGATGGGAGGTCCTCTACCTCACGTGAT
AGCTGCTAAAACAGTGGCTTTGAAGGAAGCTCTCTCTGTGGATTTCAAGAAATACGCTCATCAGGTTGTAAATAATGCTC
GTCGATTAGCAGAGAGATTTTTAAGTCATGGGCTACGTCTTTTGACGGGAGGAACAGACAACCACATGATGGTGATTGAT
TTAGGTTCTTTGGGCATTTCTGGAAAAATTGCTGAAGATATCTTGAGTTCCGTAGGAATTGCTGTGAATCGGAATTCATT
ACCTTCAGATGCTATTGGTAAGTGGGACACTTCAGGTATACGTTTAGGAACCCCTGCACTAACGACTTTGGGTATGGGTA
TCGATGAAATGGAAGAAGTTGCAGATATTATTGTGAAAGTATTGCGAAATATTCGTTTAAGTTGCCATGTTGAAGGGAGT
TCTAAGAAAAATAAAGGGGAACTTCCTGAAGCCATAGCGCAGGAAGCTAGAGATCGTGTTCGCAACTTGTTGCTGCGTTT
CCCGCTCTACCCTGAAATTGATTTAGAAGCTTTAGTTTAG

Upstream 100 bases:

>100_bases
TCTTGATCATGCGTTGATTGACATTTTTCACTTTGAAAGCTAGGCTGGTTTTTTCTGGACTTAGAGGTTCTCTCTATTAA
GGCTTCGTCTTTAGAAGTCC

Downstream 100 bases:

>100_bases
TTAGGAGAGACATTATTTTATGGCAGACGGGGAAGTTCATAAATTACGTGATATTATAGAAAAAGAGTTATTGGAAGCGC
GCAGAGTATTTTTCTCAGAG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLKVFEKFKKFAIVEIFTKVVAVVSLLHKFLENASGKKGQSLASTAYLAALDHLLNAFPSIGERIIDELKSQRSHLKMIA
SENYSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFAADCACVQPHSGADANLLAVMAILTHKV
QGPAVSKLGYKTVNELTEEEYTLLKAEMSSCVCLGPSLNSGGHLTHGNVRLNVMSKLMRCFPYDVNPDTECFDYAEISRL
AKEYKPKVLIAGYSSYSRRLNFAVLKQIAEDCGSVLWVDMAHFAGLVAGGVFVDEENPIPYADIVTTTTHKTLRGPRGGL
VLATREYESTLNKACPLMMGGPLPHVIAAKTVALKEALSVDFKKYAHQVVNNARRLAERFLSHGLRLLTGGTDNHMMVID
LGSLGISGKIAEDILSSVGIAVNRNSLPSDAIGKWDTSGIRLGTPALTTLGMGIDEMEEVADIIVKVLRNIRLSCHVEGS
SKKNKGELPEAIAQEARDRVRNLLLRFPLYPEIDLEALV

Sequences:

>Translated_519_residues
MLKVFEKFKKFAIVEIFTKVVAVVSLLHKFLENASGKKGQSLASTAYLAALDHLLNAFPSIGERIIDELKSQRSHLKMIA
SENYSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFAADCACVQPHSGADANLLAVMAILTHKV
QGPAVSKLGYKTVNELTEEEYTLLKAEMSSCVCLGPSLNSGGHLTHGNVRLNVMSKLMRCFPYDVNPDTECFDYAEISRL
AKEYKPKVLIAGYSSYSRRLNFAVLKQIAEDCGSVLWVDMAHFAGLVAGGVFVDEENPIPYADIVTTTTHKTLRGPRGGL
VLATREYESTLNKACPLMMGGPLPHVIAAKTVALKEALSVDFKKYAHQVVNNARRLAERFLSHGLRLLTGGTDNHMMVID
LGSLGISGKIAEDILSSVGIAVNRNSLPSDAIGKWDTSGIRLGTPALTTLGMGIDEMEEVADIIVKVLRNIRLSCHVEGS
SKKNKGELPEAIAQEARDRVRNLLLRFPLYPEIDLEALV
>Mature_519_residues
MLKVFEKFKKFAIVEIFTKVVAVVSLLHKFLENASGKKGQSLASTAYLAALDHLLNAFPSIGERIIDELKSQRSHLKMIA
SENYSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFAADCACVQPHSGADANLLAVMAILTHKV
QGPAVSKLGYKTVNELTEEEYTLLKAEMSSCVCLGPSLNSGGHLTHGNVRLNVMSKLMRCFPYDVNPDTECFDYAEISRL
AKEYKPKVLIAGYSSYSRRLNFAVLKQIAEDCGSVLWVDMAHFAGLVAGGVFVDEENPIPYADIVTTTTHKTLRGPRGGL
VLATREYESTLNKACPLMMGGPLPHVIAAKTVALKEALSVDFKKYAHQVVNNARRLAERFLSHGLRLLTGGTDNHMMVID
LGSLGISGKIAEDILSSVGIAVNRNSLPSDAIGKWDTSGIRLGTPALTTLGMGIDEMEEVADIIVKVLRNIRLSCHVEGS
SKKNKGELPEAIAQEARDRVRNLLLRFPLYPEIDLEALV

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=437, Percent_Identity=36.3844393592677, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI261862350, Length=437, Percent_Identity=36.3844393592677, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI261862348, Length=437, Percent_Identity=36.3844393592677, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI19923315, Length=437, Percent_Identity=36.3844393592677, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI22547186, Length=476, Percent_Identity=35.7142857142857, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI261862346, Length=437, Percent_Identity=35.4691075514874, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI22547189, Length=460, Percent_Identity=34.5652173913044, Blast_Score=229, Evalue=6e-60,
Organism=Escherichia coli, GI1788902, Length=443, Percent_Identity=41.9864559819413, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25144732, Length=437, Percent_Identity=37.7574370709382, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25144729, Length=437, Percent_Identity=37.7574370709382, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6323087, Length=440, Percent_Identity=36.1363636363636, Blast_Score=270, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6319739, Length=441, Percent_Identity=36.5079365079365, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24640005, Length=481, Percent_Identity=37.006237006237, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI221329721, Length=481, Percent_Identity=37.006237006237, Blast_Score=285, Evalue=5e-77,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 56829; Mature: 56829

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVFEKFKKFAIVEIFTKVVAVVSLLHKFLENASGKKGQSLASTAYLAALDHLLNAFPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGERIIDELKSQRSHLKMIASENYSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EWECVETAKELFAADCACVQPHSGADANLLAVMAILTHKVQGPAVSKLGYKTVNELTEEE
HHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YTLLKAEMSSCVCLGPSLNSGGHLTHGNVRLNVMSKLMRCFPYDVNPDTECFDYAEISRL
HHHHHHHHCCEEEECCCCCCCCCEEECCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AKEYKPKVLIAGYSSYSRRLNFAVLKQIAEDCGSVLWVDMAHFAGLVAGGVFVDEENPIP
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
YADIVTTTTHKTLRGPRGGLVLATREYESTLNKACPLMMGGPLPHVIAAKTVALKEALSV
HHHHEECCCHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
DFKKYAHQVVNNARRLAERFLSHGLRLLTGGTDNHMMVIDLGSLGISGKIAEDILSSVGI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCE
AVNRNSLPSDAIGKWDTSGIRLGTPALTTLGMGIDEMEEVADIIVKVLRNIRLSCHVEGS
EEECCCCCCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
SKKNKGELPEAIAQEARDRVRNLLLRFPLYPEIDLEALV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure
MLKVFEKFKKFAIVEIFTKVVAVVSLLHKFLENASGKKGQSLASTAYLAALDHLLNAFPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGERIIDELKSQRSHLKMIASENYSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EWECVETAKELFAADCACVQPHSGADANLLAVMAILTHKVQGPAVSKLGYKTVNELTEEE
HHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YTLLKAEMSSCVCLGPSLNSGGHLTHGNVRLNVMSKLMRCFPYDVNPDTECFDYAEISRL
HHHHHHHHCCEEEECCCCCCCCCEEECCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AKEYKPKVLIAGYSSYSRRLNFAVLKQIAEDCGSVLWVDMAHFAGLVAGGVFVDEENPIP
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
YADIVTTTTHKTLRGPRGGLVLATREYESTLNKACPLMMGGPLPHVIAAKTVALKEALSV
HHHHEECCCHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
DFKKYAHQVVNNARRLAERFLSHGLRLLTGGTDNHMMVIDLGSLGISGKIAEDILSSVGI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCE
AVNRNSLPSDAIGKWDTSGIRLGTPALTTLGMGIDEMEEVADIIVKVLRNIRLSCHVEGS
EEECCCCCCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCC
SKKNKGELPEAIAQEARDRVRNLLLRFPLYPEIDLEALV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]