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The map label for this gene is murE

Identifier: 15835951

GI number: 15835951

Start: 462659

End: 464110

Strand: Reverse

Name: murE

Synonym: CPj0418

Alternate gene names: 15835951

Gene position: 464110-462659 (Counterclockwise)

Preceding gene: 15835952

Following gene: 15835950

Centisome position: 37.84

GC content: 42.7

Gene sequence:

>1452_bases
ATGGATTTAAAAGAGTTACTCCATGGGGTTCAAGCTAAAATCTACGGGAAAGTTCGCCCTCTTGAAGTGCGCAACTTGAC
ACGTGATTCCCGTTGTGTGAGTGTTGGCGACATTTTTATAGCCCATAAGGGACAGCGCTACGACGGAAATGATTTTGCTG
TCGATGCTTTAGCTAATGGAGCAATTGCCATTGCTTCTTCACTATACAATCCGTTTCTTTCCGTTGTTCAGATCATCACT
CCTAATCTCGAAGAATTAGAGGCTGAGCTTTCTGCAAAGTATTACGAATACCCTTCAAGTAAGCTCCATACCATTGGGGT
GACTGGAACCAATGGGAAAACTACAGTTACATGTTTGATTAAAGCTTTATTGGATAGCTATCAAAAACCTTCAGGGCTTT
TAGGAACCATAGAGCATATCTTAGGAGAGGGGGTGATTAAAGATGGGTTTACTACACCTACACCCGCTCTTTTACAGAAG
TATTTAGCCACTATGGTACGTCAAAATAGAGACGCTGTTGTTATGGAAGTCTCTTCTATAGGACTTGCCTCTGGAAGAGT
AGCCTATACCAATTTTGATACAGCAGTTCTGACTAATATTACCTTAGATCATCTCGATTTTCATGGCACATTTGAAACCT
ATGTTGCGGCGAAAGCCAAGCTTTTCTCTCTCGTGCCCCCTTCGGGAATGGTTGTTATCAACACAGACTCTCCCTACGCT
TCTCAGTGTATTGAGAGTGCAAAGGCACCGGTCATCACTTATGGTATAGAGAGTGCTGCTGACTACCGAGCCACCGATAT
CCAACTTTCTTCCTCGGGAACAAAGTATACCTTGGTGTACGGGGACCAAAAAATTGCGTGCTCTTCCTCATTTATTGGAA
AGTACAACGTCTATAACCTACTTGCTGCGATCTCTACAGTACATGCAAGTTTGCGTTGCGATCTTGAAGATTTGCTAGAA
AAGATAGGCTTGTGTCAACCTCCTCCAGGTCGTTTGGATCCTGTACTTATGGGTCCCTGCCCTGTATATATTGATTATGC
ACACACCCCCGATGCTTTAGACAATGTCTTAACAGGATTGCATGAGTTACTTCCTGAGGGGGGAAGACTGATTGTTGTTT
TTGGTTGCGGTGGAGATAGAGATCGCAGTAAACGGAAGTTGATGGCCCAGGTGGTAGAGCGTTATGGTTTTGCTGTTGTA
ACTTCAGATAACCCTAGGAGCGAGCCTCCTGAAGATATTGTGAATGAAATTTGTGATGGGTTTTATTCAAAAAACTATTT
CATCGAAATCGACAGAAAACAAGCAATTACATATGCTCTGTCTATTGCCTCAGATAGAGATATAGTGTTAATAGCGGGAA
AAGGGCATGAAGCTTACCAAATATTTAAACACCAAACAGTTGCGTTCGATGATAAGCAGACTGTTTGTGAGGTACTCGCT
TCCTATGTCTAA

Upstream 100 bases:

>100_bases
AAAGAAATCCTTTAAGCATTTTTTAAAAATCAGAGTGAGAGCATGCCCCTAGAGGGCTTTTTATGAAAAAAGTTGTTTTT
CAATAGTCCCTGGAGCGTAA

Downstream 100 bases:

>100_bases
GCAACTGTCGTTTTTTGCTTTATGTGTGTTAGGAAGTCACCCGATTTTTGCTCAAACACCGAATCCTCCTCAGCGTGTAC
GACGCAGTGAGGTTATATTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MDLKELLHGVQAKIYGKVRPLEVRNLTRDSRCVSVGDIFIAHKGQRYDGNDFAVDALANGAIAIASSLYNPFLSVVQIIT
PNLEELEAELSAKYYEYPSSKLHTIGVTGTNGKTTVTCLIKALLDSYQKPSGLLGTIEHILGEGVIKDGFTTPTPALLQK
YLATMVRQNRDAVVMEVSSIGLASGRVAYTNFDTAVLTNITLDHLDFHGTFETYVAAKAKLFSLVPPSGMVVINTDSPYA
SQCIESAKAPVITYGIESAADYRATDIQLSSSGTKYTLVYGDQKIACSSSFIGKYNVYNLLAAISTVHASLRCDLEDLLE
KIGLCQPPPGRLDPVLMGPCPVYIDYAHTPDALDNVLTGLHELLPEGGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAVV
TSDNPRSEPPEDIVNEICDGFYSKNYFIEIDRKQAITYALSIASDRDIVLIAGKGHEAYQIFKHQTVAFDDKQTVCEVLA
SYV

Sequences:

>Translated_483_residues
MDLKELLHGVQAKIYGKVRPLEVRNLTRDSRCVSVGDIFIAHKGQRYDGNDFAVDALANGAIAIASSLYNPFLSVVQIIT
PNLEELEAELSAKYYEYPSSKLHTIGVTGTNGKTTVTCLIKALLDSYQKPSGLLGTIEHILGEGVIKDGFTTPTPALLQK
YLATMVRQNRDAVVMEVSSIGLASGRVAYTNFDTAVLTNITLDHLDFHGTFETYVAAKAKLFSLVPPSGMVVINTDSPYA
SQCIESAKAPVITYGIESAADYRATDIQLSSSGTKYTLVYGDQKIACSSSFIGKYNVYNLLAAISTVHASLRCDLEDLLE
KIGLCQPPPGRLDPVLMGPCPVYIDYAHTPDALDNVLTGLHELLPEGGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAVV
TSDNPRSEPPEDIVNEICDGFYSKNYFIEIDRKQAITYALSIASDRDIVLIAGKGHEAYQIFKHQTVAFDDKQTVCEVLA
SYV
>Mature_483_residues
MDLKELLHGVQAKIYGKVRPLEVRNLTRDSRCVSVGDIFIAHKGQRYDGNDFAVDALANGAIAIASSLYNPFLSVVQIIT
PNLEELEAELSAKYYEYPSSKLHTIGVTGTNGKTTVTCLIKALLDSYQKPSGLLGTIEHILGEGVIKDGFTTPTPALLQK
YLATMVRQNRDAVVMEVSSIGLASGRVAYTNFDTAVLTNITLDHLDFHGTFETYVAAKAKLFSLVPPSGMVVINTDSPYA
SQCIESAKAPVITYGIESAADYRATDIQLSSSGTKYTLVYGDQKIACSSSFIGKYNVYNLLAAISTVHASLRCDLEDLLE
KIGLCQPPPGRLDPVLMGPCPVYIDYAHTPDALDNVLTGLHELLPEGGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAVV
TSDNPRSEPPEDIVNEICDGFYSKNYFIEIDRKQAITYALSIASDRDIVLIAGKGHEAYQIFKHQTVAFDDKQTVCEVLA
SYV

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=479, Percent_Identity=36.9519832985386, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786274, Length=357, Percent_Identity=24.9299719887955, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CHLPN (Q9Z8C5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D72080
- PIR:   H86542
- RefSeq:   NP_224618.1
- RefSeq:   NP_300475.1
- RefSeq:   NP_444885.1
- RefSeq:   NP_876708.1
- ProteinModelPortal:   Q9Z8C5
- SMR:   Q9Z8C5
- GeneID:   1467115
- GeneID:   894904
- GeneID:   919161
- GeneID:   962867
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0336
- KEGG:   cpn:CPn0418
- KEGG:   cpt:CpB0434
- TIGR:   CP_0336
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   Q9Z8C5
- ProtClustDB:   PRK00139
- BioCyc:   CPNE115711:CP_0336-MONOMER
- BioCyc:   CPNE115713:CPN0418-MONOMER
- BioCyc:   CPNE138677:CPJ0418-MONOMER
- BioCyc:   CPNE182082:CPB0434-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 52721; Mature: 52721

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 178-178 BINDING 186-186 BINDING 380-380 BINDING 453-453 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKELLHGVQAKIYGKVRPLEVRNLTRDSRCVSVGDIFIAHKGQRYDGNDFAVDALANG
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECEEEEEECCCCCCCCCEEHHHHCCC
AIAIASSLYNPFLSVVQIITPNLEELEAELSAKYYEYPSSKLHTIGVTGTNGKTTVTCLI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
KALLDSYQKPSGLLGTIEHILGEGVIKDGFTTPTPALLQKYLATMVRQNRDAVVMEVSSI
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
GLASGRVAYTNFDTAVLTNITLDHLDFHGTFETYVAAKAKLFSLVPPSGMVVINTDSPYA
CCCCCEEEEEECCCCCEEEEEHCCCCCCCCHHHHHHHHHHHEEECCCCCEEEEECCCCHH
SQCIESAKAPVITYGIESAADYRATDIQLSSSGTKYTLVYGDQKIACSSSFIGKYNVYNL
HHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCEEEECCCCCCCHHHHHH
LAAISTVHASLRCDLEDLLEKIGLCQPPPGRLDPVLMGPCPVYIDYAHTPDALDNVLTGL
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHH
HELLPEGGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAVVTSDNPRSEPPEDIVNEICDG
HHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
FYSKNYFIEIDRKQAITYALSIASDRDIVLIAGKGHEAYQIFKHQTVAFDDKQTVCEVLA
HCCCCEEEEEECHHEEEEEEEECCCCCEEEEECCCCHHHHHHHCCEEEECCHHHHHHHHH
SYV
HCC
>Mature Secondary Structure
MDLKELLHGVQAKIYGKVRPLEVRNLTRDSRCVSVGDIFIAHKGQRYDGNDFAVDALANG
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECEEEEEECCCCCCCCCEEHHHHCCC
AIAIASSLYNPFLSVVQIITPNLEELEAELSAKYYEYPSSKLHTIGVTGTNGKTTVTCLI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
KALLDSYQKPSGLLGTIEHILGEGVIKDGFTTPTPALLQKYLATMVRQNRDAVVMEVSSI
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
GLASGRVAYTNFDTAVLTNITLDHLDFHGTFETYVAAKAKLFSLVPPSGMVVINTDSPYA
CCCCCEEEEEECCCCCEEEEEHCCCCCCCCHHHHHHHHHHHEEECCCCCEEEEECCCCHH
SQCIESAKAPVITYGIESAADYRATDIQLSSSGTKYTLVYGDQKIACSSSFIGKYNVYNL
HHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCEEEECCCCCCCHHHHHH
LAAISTVHASLRCDLEDLLEKIGLCQPPPGRLDPVLMGPCPVYIDYAHTPDALDNVLTGL
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEECCCCCHHHHHHHHHH
HELLPEGGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAVVTSDNPRSEPPEDIVNEICDG
HHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
FYSKNYFIEIDRKQAITYALSIASDRDIVLIAGKGHEAYQIFKHQTVAFDDKQTVCEVLA
HCCCCEEEEEECHHEEEEEEEECCCCCEEEEECCCCHHHHHHHCCEEEECCHHHHHHHHH
SYV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362