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The map label for this gene is amiA

Identifier: 15835950

GI number: 15835950

Start: 461950

End: 462744

Strand: Reverse

Name: amiA

Synonym: CPj0417

Alternate gene names: 15835950

Gene position: 462744-461950 (Counterclockwise)

Preceding gene: 15835951

Following gene: 15835949

Centisome position: 37.73

GC content: 42.77

Gene sequence:

>795_bases
ATGAAGCTTACCAAATATTTAAACACCAAACAGTTGCGTTCGATGATAAGCAGACTGTTTGTGAGGTACTCGCTTCCTAT
GTCTAAGCAACTGTCGTTTTTTGCTTTATGTGTGTTAGGAAGTCACCCGATTTTTGCTCAAACACCGAATCCTCCTCAGC
GTGTACGACGCAGTGAGGTTATATTTATAGATCCTGGACACGGGGGAAAAGATCAAGGCACGGCAAGTAAGGAACTTCAT
TATGAAGAGAAGTCCCTGACCCTGTCTCTTGCTTTGACGGTTCAAAGTTACTTAAAGCGGATGGGTTATAAACCTCAGCT
AACCCGATCTTCTGATGTATACGTTGACTTAGGGAAACGCGTTGCTTTGTCGAACCGTGGGCAGGGGGATGTCTTTATCA
GCATCCACTGTAATCATTCTTCAAACGCAGCAGCCTTTGGCACCGAAGTATATTTTTATAATGGTAAGGTCGGATCTCCG
ACTAGGAATCGCATGTCAGAAGTACTGGGAAAAAACATTTTAGCTGCTATGGAAAAAAATGGCATTTTGAAGTCTCGAGG
TTTGAAAACTGCGAACTTTGTTGTGATTAGAGATACTTCTATGCCTGCAGTTTTGGTGGAAACCGGGTTTTTATCCAATA
GTCGTGAACGTGCGGACCTGCAAGATGCTCGCTATCGTATGCATGTAGCGAAAGGCATCGCCGAGGGAGTTCATAATTTT
CTTTCTGGACCTAGTTTTCAGAAACCAAAACAGAATATCGCTAAAATACGTAAACCACAGATACAAGCAAATTAG

Upstream 100 bases:

>100_bases
TCAAAAAACTATTTCATCGAAATCGACAGAAAACAAGCAATTACATATGCTCTGTCTATTGCCTCAGATAGAGATATAGT
GTTAATAGCGGGAAAAGGGC

Downstream 100 bases:

>100_bases
TACTTTAGGAGTTAAAGGCAAAAAATCGTCCTCGATGCGATTCGAACGCATGGCCTGCTGCTTAGGAGGCAACCGCTCTA
TCCTGCTGAGCTACGAGGAC

Product: N-acetylmuramoyl alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKLTKYLNTKQLRSMISRLFVRYSLPMSKQLSFFALCVLGSHPIFAQTPNPPQRVRRSEVIFIDPGHGGKDQGTASKELH
YEEKSLTLSLALTVQSYLKRMGYKPQLTRSSDVYVDLGKRVALSNRGQGDVFISIHCNHSSNAAAFGTEVYFYNGKVGSP
TRNRMSEVLGKNILAAMEKNGILKSRGLKTANFVVIRDTSMPAVLVETGFLSNSRERADLQDARYRMHVAKGIAEGVHNF
LSGPSFQKPKQNIAKIRKPQIQAN

Sequences:

>Translated_264_residues
MKLTKYLNTKQLRSMISRLFVRYSLPMSKQLSFFALCVLGSHPIFAQTPNPPQRVRRSEVIFIDPGHGGKDQGTASKELH
YEEKSLTLSLALTVQSYLKRMGYKPQLTRSSDVYVDLGKRVALSNRGQGDVFISIHCNHSSNAAAFGTEVYFYNGKVGSP
TRNRMSEVLGKNILAAMEKNGILKSRGLKTANFVVIRDTSMPAVLVETGFLSNSRERADLQDARYRMHVAKGIAEGVHNF
LSGPSFQKPKQNIAKIRKPQIQAN
>Mature_264_residues
MKLTKYLNTKQLRSMISRLFVRYSLPMSKQLSFFALCVLGSHPIFAQTPNPPQRVRRSEVIFIDPGHGGKDQGTASKELH
YEEKSLTLSLALTVQSYLKRMGYKPQLTRSSDVYVDLGKRVALSNRGQGDVFISIHCNHSSNAAAFGTEVYFYNGKVGSP
TRNRMSEVLGKNILAAMEKNGILKSRGLKTANFVVIRDTSMPAVLVETGFLSNSRERADLQDARYRMHVAKGIAEGVHNF
LSGPSFQKPKQNIAKIRKPQIQAN

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=31.2775330396476, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082163, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29478; Mature: 29478

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKYLNTKQLRSMISRLFVRYSLPMSKQLSFFALCVLGSHPIFAQTPNPPQRVRRSEV
CCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCE
IFIDPGHGGKDQGTASKELHYEEKSLTLSLALTVQSYLKRMGYKPQLTRSSDVYVDLGKR
EEEECCCCCCCCCCCCHHHEECCHHEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCE
VALSNRGQGDVFISIHCNHSSNAAAFGTEVYFYNGKVGSPTRNRMSEVLGKNILAAMEKN
EEECCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GILKSRGLKTANFVVIRDTSMPAVLVETGFLSNSRERADLQDARYRMHVAKGIAEGVHNF
CCCHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSGPSFQKPKQNIAKIRKPQIQAN
HCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLTKYLNTKQLRSMISRLFVRYSLPMSKQLSFFALCVLGSHPIFAQTPNPPQRVRRSEV
CCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCE
IFIDPGHGGKDQGTASKELHYEEKSLTLSLALTVQSYLKRMGYKPQLTRSSDVYVDLGKR
EEEECCCCCCCCCCCCHHHEECCHHEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCE
VALSNRGQGDVFISIHCNHSSNAAAFGTEVYFYNGKVGSPTRNRMSEVLGKNILAAMEKN
EEECCCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GILKSRGLKTANFVVIRDTSMPAVLVETGFLSNSRERADLQDARYRMHVAKGIAEGVHNF
CCCHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSGPSFQKPKQNIAKIRKPQIQAN
HCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]