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The map label for this gene is sucB_1

Identifier: 15835910

GI number: 15835910

Start: 422053

End: 423147

Strand: Reverse

Name: sucB_1

Synonym: CPj0377

Alternate gene names: 15835910

Gene position: 423147-422053 (Counterclockwise)

Preceding gene: 15835911

Following gene: 15835896

Centisome position: 34.5

GC content: 44.47

Gene sequence:

>1095_bases
ATGACTACAGAAGTACGCATTCCTAATATTGCAGAGTCGATTAGCGAGGTGACCGTAGCTTCCTTGTTAGTTACAGAGGG
TGCTCTGATTCAAGAAAACCAGGGCTTACTAGAAATTGAAAGTGATAAGGTAAATCAGCTCATTTATGCCCCAGTATCGG
GAAGAATTTTCTGGGAGGTTTCAGAAGGCGATGTTGTTCCTGTAGGGGGGGTAGTGGGAAAAATAGAGCCCGCAGGTGAA
GGGGAAGAGCTTGGAGATTCTCAGTCTAAAGAGACTATAGAAGCTGAGATCATTTGCTTTCCTCAGTCTGGGGTGCGTCA
GTCTCCTCCAGAGAATAAAACGTTTATTCCTCTTCGTGATCAGATGGACCAAGGATCCCAAGGTCTTTCTGCAGGAGATC
GAGGAGAAACTCGAGAACGCATGACCTCGATTCGTAAGACAATTTCGCGGCGTCTTTTGTCTGCTTTACATGAGTCTGCG
ATGCTCACGACATTCAATGAGGTCTATATGACACCTCTTTTTCATTTGCGAAAGGAAAAACAAGAAGAGTTTCTATCTCG
ATATGGGGTGAAGTTAGGATTTATGTCTTTCTTTGTGAAAGCTGTCTTAGAGGCTTTGAAGGCATATCCACGAGTGAACG
CCTATATTGATGGCGAGGAGATTGTTTACCGTCACTATTATGACATTTCTATTGCTGTAGGTATCGATCGAGGACTTGTG
GTTCCTGTGATACGCGATTGCGATAAACTTTCTAACGGGGAGATTGAGCAGAAACTCGCAGATCTTGCCCTTCGGGCTCG
TGAAGGCCTACTTGCAATAGCGGAGCTTGAGGGAGGAGGTTTCACAATTACCAATGGAGGCGTATATGGATCGCTACTTT
CGACTCCCATTATCAATCCCCCGCAAGTGGGGATTTTGGGGATGCATAAGATAGAAAAGCGCCCCGTTGTTCTTGATAAT
GAAATTGTAATTGCAGATATGATGTATGTCGCTTTAAGCTATGATCATCGTCTTATTGATGGGAAAGAGGCTGTTGGGTT
TTTAGTCAAAGTGAAAGAAGGCCTAGAGAATCCTGCCTCATTACTCGACTTGTAA

Upstream 100 bases:

>100_bases
AGGACGTCCTCGGAGTAGTTCCACAGCTTCTGGATCAGCGAAGCTCAGTCGTCAAGAGCTGGTCACGTGTATGGAAACCC
TCTTTTCTTTAAGGTAAATT

Downstream 100 bases:

>100_bases
TTTCTCTGATTCTCATAAAGGCTCTTTTAGAGCCTTTTCAGATTTTTTAACCTCTTTTCTTATCATGAAAAGGATTGCAC
TCATCTTGAGTAAGGAACAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTTEVRIPNIAESISEVTVASLLVTEGALIQENQGLLEIESDKVNQLIYAPVSGRIFWEVSEGDVVPVGGVVGKIEPAGE
GEELGDSQSKETIEAEIICFPQSGVRQSPPENKTFIPLRDQMDQGSQGLSAGDRGETRERMTSIRKTISRRLLSALHESA
MLTTFNEVYMTPLFHLRKEKQEEFLSRYGVKLGFMSFFVKAVLEALKAYPRVNAYIDGEEIVYRHYYDISIAVGIDRGLV
VPVIRDCDKLSNGEIEQKLADLALRAREGLLAIAELEGGGFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVLDN
EIVIADMMYVALSYDHRLIDGKEAVGFLVKVKEGLENPASLLDL

Sequences:

>Translated_364_residues
MTTEVRIPNIAESISEVTVASLLVTEGALIQENQGLLEIESDKVNQLIYAPVSGRIFWEVSEGDVVPVGGVVGKIEPAGE
GEELGDSQSKETIEAEIICFPQSGVRQSPPENKTFIPLRDQMDQGSQGLSAGDRGETRERMTSIRKTISRRLLSALHESA
MLTTFNEVYMTPLFHLRKEKQEEFLSRYGVKLGFMSFFVKAVLEALKAYPRVNAYIDGEEIVYRHYYDISIAVGIDRGLV
VPVIRDCDKLSNGEIEQKLADLALRAREGLLAIAELEGGGFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVLDN
EIVIADMMYVALSYDHRLIDGKEAVGFLVKVKEGLENPASLLDL
>Mature_363_residues
TTEVRIPNIAESISEVTVASLLVTEGALIQENQGLLEIESDKVNQLIYAPVSGRIFWEVSEGDVVPVGGVVGKIEPAGEG
EELGDSQSKETIEAEIICFPQSGVRQSPPENKTFIPLRDQMDQGSQGLSAGDRGETRERMTSIRKTISRRLLSALHESAM
LTTFNEVYMTPLFHLRKEKQEEFLSRYGVKLGFMSFFVKAVLEALKAYPRVNAYIDGEEIVYRHYYDISIAVGIDRGLVV
PVIRDCDKLSNGEIEQKLADLALRAREGLLAIAELEGGGFTITNGGVYGSLLSTPIINPPQVGILGMHKIEKRPVVLDNE
IVIADMMYVALSYDHRLIDGKEAVGFLVKVKEGLENPASLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=237, Percent_Identity=52.3206751054852, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI31711992, Length=224, Percent_Identity=32.5892857142857, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI110671329, Length=203, Percent_Identity=35.9605911330049, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI203098816, Length=227, Percent_Identity=30.8370044052863, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI203098753, Length=227, Percent_Identity=30.8370044052863, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=34.7305389221557, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=229, Percent_Identity=58.9519650655022, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1786305, Length=214, Percent_Identity=35.0467289719626, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=389, Percent_Identity=39.8457583547558, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=27.0983213429257, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=252, Percent_Identity=31.7460317460317, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17538894, Length=223, Percent_Identity=31.8385650224215, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=393, Percent_Identity=38.1679389312977, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324258, Length=228, Percent_Identity=29.8245614035088, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645909, Length=228, Percent_Identity=53.5087719298246, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI18859875, Length=225, Percent_Identity=36, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=28.82096069869, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=28.82096069869, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 40066; Mature: 39934

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEVRIPNIAESISEVTVASLLVTEGALIQENQGLLEIESDKVNQLIYAPVSGRIFWEV
CCCCEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCEEEEEECCCEEEEEE
SEGDVVPVGGVVGKIEPAGEGEELGDSQSKETIEAEIICFPQSGVRQSPPENKTFIPLRD
CCCCEEEECCEEECCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECHH
QMDQGSQGLSAGDRGETRERMTSIRKTISRRLLSALHESAMLTTFNEVYMTPLFHLRKEK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEFLSRYGVKLGFMSFFVKAVLEALKAYPRVNAYIDGEEIVYRHYYDISIAVGIDRGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEEECCCCEE
VPVIRDCDKLSNGEIEQKLADLALRAREGLLAIAELEGGGFTITNGGVYGSLLSTPIINP
EHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCEEHHHHHCCCCCC
PQVGILGMHKIEKRPVVLDNEIVIADMMYVALSYDHRLIDGKEAVGFLVKVKEGLENPAS
CCCCEEEHHHHCCCCEEECCCCHHHHHHHHHHHCCCEECCCHHHHHHHHEEHHCCCCCHH
LLDL
HHCC
>Mature Secondary Structure 
TTEVRIPNIAESISEVTVASLLVTEGALIQENQGLLEIESDKVNQLIYAPVSGRIFWEV
CCCEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCEEEEEECCCEEEEEE
SEGDVVPVGGVVGKIEPAGEGEELGDSQSKETIEAEIICFPQSGVRQSPPENKTFIPLRD
CCCCEEEECCEEECCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECHH
QMDQGSQGLSAGDRGETRERMTSIRKTISRRLLSALHESAMLTTFNEVYMTPLFHLRKEK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEFLSRYGVKLGFMSFFVKAVLEALKAYPRVNAYIDGEEIVYRHYYDISIAVGIDRGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEEECCCCEE
VPVIRDCDKLSNGEIEQKLADLALRAREGLLAIAELEGGGFTITNGGVYGSLLSTPIINP
EHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCEEHHHHHCCCCCC
PQVGILGMHKIEKRPVVLDNEIVIADMMYVALSYDHRLIDGKEAVGFLVKVKEGLENPAS
CCCCEEEHHHHCCCCEEECCCCHHHHHHHHHHHCCCEECCCHHHHHHHHEEHHCCCCCHH
LLDL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA