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The map label for this gene is tyrS

Identifier: 15835894

GI number: 15835894

Start: 405088

End: 406356

Strand: Reverse

Name: tyrS

Synonym: CPj0361

Alternate gene names: 15835894

Gene position: 406356-405088 (Counterclockwise)

Preceding gene: 15835895

Following gene: 15835893

Centisome position: 33.13

GC content: 41.53

Gene sequence:

>1269_bases
TTGGCAATTCTTAATTATGTACGATCACTCATGCAATCCTGGTTACAATCTTTACAAGAGCGAAATATTTTAGAGAATTT
TACCGCAGGTTTGGAATCCGTAGAGGGACCTATCGCCGCTTATTTAGGATTTGATCCTACCGCACCTGCTCTACATATTG
GTCATTGGATTGGGATTTGTTTCTTGAAGAGACTCGCTGCTCTGGGGATTACCCCCATAGCTTTAGTCGGGGGAGCCACA
GGTATGGTTGGAGATCCCTCAGGGAAACAGAGCGAGAGATCGTTACTTCAGACAAGTGAAGTTTTTGATAACAGTCAAAA
GATCACGGCGTGTCTCCAGCGCTATCTTCCCGGGGTGACTCTTGTAAATAATGCAGACTGGTTGCAGGAGATCTCCCTGA
TTGATTTCTTAAGGGATATAGGAAAACACTTTCGTTTAGGCCAAATGCTAGTGAAAGATACAATAAAGCAGCGGGTGCAT
TCTGATGAAGGAATTAGCTATACCGAGTTTAGCTATTTAATCCTGCAATCCTATGATTTTTATCACTTATTTAAAAATTA
TGGCACGATCTTGCAGTGCGGTGGTAGCGATCAGTGGGGGAATATTACTTCAGGAATCGATTTTATTCGCCGTAAAGGGT
TGGGTCAGGCCTACGGCCTTACCTATCCTTTATTAACGAATGCTCAGGGGAAAAAAATAGGGAAAACAGAGTCGGGAACT
GTATGGCTCGATTCAGATTTAACCTCTCCTTTTGAGCTGTACCAATACTTACTCCGTTTGCCCGATGATACCATCCCTAA
AATTGCTCGTACGTTAACTTTATTGAGCAATGAAGAAATTCAAGATATTGATAGGCGTGTACAGACGGATCCAGTTGCAG
TGAAGGAATTTGTAGCCCAAGATATCTTAAGTGCTATTCATGGAGATCTAGGGCTTGAAGAGGCTCTTTCTGTAACTCGT
AGCATGCATCCAGGGAATCTTTCATCCTTATCGGAAAAAGATTTTCATGAATTGTTTGCAGGAGGGATGGGGGCCTCATT
GGATAAATCCGAGGTGTTAGGGAAACGTTGGTTAGGCCTATTTCTTGTTTTGGGACTATGTAAATCTAAAGGGGAAATTC
GAAGGCTAATTGAACAAAAAGGGGTATATATTAATAATGTGCCCATCGCTAATGAGCATAGTGTTTGTGAAGAACAAGAC
ATCTGTTATGGTCACTATGTGTTGTTGGCTCAAGGTAAAAAACGAAAGCTTGTTCTATATTTAAATTAG

Upstream 100 bases:

>100_bases
TGGTATCTCAGCAAAGGAATTATTCTCAGCGTACAATCTTTTCTTTATTGTTAGATAGGTGGTGCCTTGCATATAAGCTT
TAGGATCGATAAGATAGAGC

Downstream 100 bases:

>100_bases
TTTAAGGAGGCTAGGTAGCTTTGCAAACGAATATTGGTCTTATTGGCTTAGCTGTCATGGGGAAAAATCTTGTCTTAAAC
ATGATAGATCATGGTTTTTC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGAT
GMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVH
SDEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTR
SMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLGLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQD
ICYGHYVLLAQGKKRKLVLYLN

Sequences:

>Translated_422_residues
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGAT
GMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVH
SDEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTR
SMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLGLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQD
ICYGHYVLLAQGKKRKLVLYLN
>Mature_421_residues
AILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGATG
MVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHS
DEGISYTEFSYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTV
WLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTRS
MHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLGLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDI
CYGHYVLLAQGKKRKLVLYLN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=385, Percent_Identity=36.1038961038961, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1787925, Length=414, Percent_Identity=40.0966183574879, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI25145802, Length=319, Percent_Identity=40.4388714733542, Blast_Score=216, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25145805, Length=252, Percent_Identity=38.0952380952381, Blast_Score=154, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=269, Percent_Identity=37.1747211895911, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI20130383, Length=386, Percent_Identity=35.4922279792746, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47019; Mature: 46887

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGIC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FLKRLAALGITPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVT
HHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
LVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
YHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQ
EEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
DILSAIHGDLGLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLGL
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLY
HHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LN
EC
>Mature Secondary Structure 
AILNYVRSLMQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FLKRLAALGITPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVT
HHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
LVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
YHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGT
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VWLDSDLTSPFELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQ
EEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
DILSAIHGDLGLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLGL
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLY
HHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LN
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]