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The map label for this gene is rpsD

Identifier: 15835895

GI number: 15835895

Start: 406761

End: 407534

Strand: Reverse

Name: rpsD

Synonym: CPj0362

Alternate gene names: 15835895

Gene position: 407534-406761 (Counterclockwise)

Preceding gene: 15835896

Following gene: 15835894

Centisome position: 33.23

GC content: 44.19

Gene sequence:

>774_bases
GTGAAAACACAGCAAACTCAAAACATCATAGAGGTTTGGAACTTCTACTGGGAGACTCAGGAAATAGAGTATCGCGATAG
CTTAATTGAGTTCTATTTGCCTTTAGTAAAAAGTGTGGTTCATCGTTTGATTTCAGGGATGCCTTCCCATGTAAAGACCG
AGGATTTGTATGCTTCGGGTGTTGAAGGTCTCGTCCGTGCGGTGGAACGTTATAATCCTGAGAGAAGTCGTCGTTTTGAA
GGTTATGCGGTATTTCTGATTAAGGCTGCCATTATTGATGATCTGCGTAAGCAAGACTGGGTTCCTCGTAGTGTCCATCA
AAAAGCGAATAAATTGTCAGGAGCTATGGATTCTCTTCGCCAGTCTTTAGGCAAGGAACCCACGGATCTTGAACTGTGTG
AGTATCTCAATATTTCGCAACAAGAGCTTTCGGGATGGTTTGTATCTGCCCGTCCTGCATTAATCGTGTCTCTGAATGAA
GAGTGGCCTTCACAAAGTGATGAAGGAGCCGGAATGGCTCTTGAAGAGAGAATCCCCGATGAACGTGCCGAGACAGGGTA
CGATGTTGTAGATAAACAAGAATTTTCTTTATGTTTAGCCAATGCGATTCAGGAACTTGAGGAAAAGGAACGCAAGGTCA
TGGCCCTGTACTACTATGAAGAACTTGTCCTTAAGGAAATCGGTAAGGTCCTTGGGGTAAGTGAGTCTCGCGTCTCTCAA
ATTCACTCTAAAGCATTGCTTAAGCTTCGCGCAGCACTCTCTGCATTTCGATAA

Upstream 100 bases:

>100_bases
CTGTAAGCACTATTACTTTCGTATTTTTTTTGTTGGTGTAAATATTGTTTAAAAATTTTTTTTGAATTAATCTAATAAAT
AACTAGATAAAAAAAAATTT

Downstream 100 bases:

>100_bases
ATACAGTTCTCAGGTTTTAAGAGCAGTCCTAGAGCTAGGAGAAGCCCTCCTACGACACAGAGTAATCCGTAAAGAATTTG
TTTAGTCAACGCCATAACAA

Product: RNA polymerase sigma factor sigma-28

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKTQQTQNIIEVWNFYWETQEIEYRDSLIEFYLPLVKSVVHRLISGMPSHVKTEDLYASGVEGLVRAVERYNPERSRRFE
GYAVFLIKAAIIDDLRKQDWVPRSVHQKANKLSGAMDSLRQSLGKEPTDLELCEYLNISQQELSGWFVSARPALIVSLNE
EWPSQSDEGAGMALEERIPDERAETGYDVVDKQEFSLCLANAIQELEEKERKVMALYYYEELVLKEIGKVLGVSESRVSQ
IHSKALLKLRAALSAFR

Sequences:

>Translated_257_residues
MKTQQTQNIIEVWNFYWETQEIEYRDSLIEFYLPLVKSVVHRLISGMPSHVKTEDLYASGVEGLVRAVERYNPERSRRFE
GYAVFLIKAAIIDDLRKQDWVPRSVHQKANKLSGAMDSLRQSLGKEPTDLELCEYLNISQQELSGWFVSARPALIVSLNE
EWPSQSDEGAGMALEERIPDERAETGYDVVDKQEFSLCLANAIQELEEKERKVMALYYYEELVLKEIGKVLGVSESRVSQ
IHSKALLKLRAALSAFR
>Mature_257_residues
MKTQQTQNIIEVWNFYWETQEIEYRDSLIEFYLPLVKSVVHRLISGMPSHVKTEDLYASGVEGLVRAVERYNPERSRRFE
GYAVFLIKAAIIDDLRKQDWVPRSVHQKANKLSGAMDSLRQSLGKEPTDLELCEYLNISQQELSGWFVSARPALIVSLNE
EWPSQSDEGAGMALEERIPDERAETGYDVVDKQEFSLCLANAIQELEEKERKVMALYYYEELVLKEIGKVLGVSESRVSQ
IHSKALLKLRAALSAFR

Specific function: The level of this sigma factor may be crucial in determining the developmental fate of hyphae [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=243, Percent_Identity=36.2139917695473, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789448, Length=203, Percent_Identity=27.0935960591133, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789098, Length=245, Percent_Identity=26.9387755102041, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29550; Mature: 29550

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQQTQNIIEVWNFYWETQEIEYRDSLIEFYLPLVKSVVHRLISGMPSHVKTEDLYASG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VEGLVRAVERYNPERSRRFEGYAVFLIKAAIIDDLRKQDWVPRSVHQKANKLSGAMDSLR
HHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QSLGKEPTDLELCEYLNISQQELSGWFVSARPALIVSLNEEWPSQSDEGAGMALEERIPD
HHHCCCCCHHHHHHHHCCCHHHHCCEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHCCC
ERAETGYDVVDKQEFSLCLANAIQELEEKERKVMALYYYEELVLKEIGKVLGVSESRVSQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IHSKALLKLRAALSAFR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTQQTQNIIEVWNFYWETQEIEYRDSLIEFYLPLVKSVVHRLISGMPSHVKTEDLYASG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VEGLVRAVERYNPERSRRFEGYAVFLIKAAIIDDLRKQDWVPRSVHQKANKLSGAMDSLR
HHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QSLGKEPTDLELCEYLNISQQELSGWFVSARPALIVSLNEEWPSQSDEGAGMALEERIPD
HHHCCCCCHHHHHHHHCCCHHHHCCEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHCCC
ERAETGYDVVDKQEFSLCLANAIQELEEKERKVMALYYYEELVLKEIGKVLGVSESRVSQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IHSKALLKLRAALSAFR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2507166; 12000953 [H]