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The map label for this gene is smpB

Identifier: 15835872

GI number: 15835872

Start: 381194

End: 381649

Strand: Reverse

Name: smpB

Synonym: CPj0337

Alternate gene names: 15835872

Gene position: 381649-381194 (Counterclockwise)

Preceding gene: 15835877

Following gene: 15835868

Centisome position: 31.12

GC content: 42.98

Gene sequence:

>456_bases
ATGGCACAAAAAGAAATTGTTTCTAATCGCAAGGCTCTGCGTAACTATGAAGTTATAGAGACTTTAGAAGCAGGCATCGT
TTTGACTGGGACTGAGATTAAGTCGTTGCGCGATCATGGGGGAAACCTCGGTGATGCTTATGTCATTGTTTCTAAAGGTG
AGGGGTGGTTATTAAACGCGAGTATTGCTCCCTATCGGTTTGGAAATATCTATAACCATGAGGAGCGTCGTAAACGTAAA
CTCCTTCTTCATAGATATGAACTTCGTAAGTTAGAGGGTAAGATTGCTCAAAAGGGCATGACTTTGATTCCTCTGGGAAT
GTTTCTGAGTCGCGGCTATGTTAAGGTACGTTTGGGTTGTTGTCGTGGGAAAAAAGCTTATGATAAGCGTCGTACGATCA
TAGAAAGAGAAAAGGAACGTGAAGTTGCCGCTGCTATGAAGAGGCGCCATCATTGA

Upstream 100 bases:

>100_bases
CCCTGGAAATCAAGGAAAGAGGATTCTAATCATTGTCTTAAGAAGGAAAAATTGCTTTTTGTTATACTGGTTTTTGTCCC
CAATTATGGGGGAGGATCTT

Downstream 100 bases:

>100_bases
TATAGGTTAGGATATGGTGTTCTTCAGCCCACTGTTTTGCTTCTATTTTAGAATCAAAAGTCATGAGGACTGTGGCAATA
GCGTCGGCGTATGCGCAGCT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYRFGNIYNHEERRKRK
LLLHRYELRKLEGKIAQKGMTLIPLGMFLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAMKRRHH

Sequences:

>Translated_151_residues
MAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYRFGNIYNHEERRKRK
LLLHRYELRKLEGKIAQKGMTLIPLGMFLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAMKRRHH
>Mature_150_residues
AQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNASIAPYRFGNIYNHEERRKRKL
LLHRYELRKLEGKIAQKGMTLIPLGMFLSRGYVKVRLGCCRGKKAYDKRRTIIEREKEREVAAAMKRRHH

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=146, Percent_Identity=37.6712328767123, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CHLPN (Q9Z8K1)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86533
- PIR:   G72090
- PIR:   H81578
- RefSeq:   NP_224542.1
- RefSeq:   NP_300396.1
- RefSeq:   NP_444969.1
- RefSeq:   NP_876622.1
- ProteinModelPortal:   Q9Z8K1
- SMR:   Q9Z8K1
- GeneID:   1467029
- GeneID:   895127
- GeneID:   919111
- GeneID:   962715
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0421
- KEGG:   cpn:CPn0337
- KEGG:   cpt:CpB0345
- TIGR:   CP_0421
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   CPNE115711:CP_0421-MONOMER
- BioCyc:   CPNE115713:CPN0337-MONOMER
- BioCyc:   CPNE138677:CPJ0337-MONOMER
- BioCyc:   CPNE182082:CPB0345-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17450; Mature: 17319

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNA
CCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCEEEEEECCCCEEEEC
SIAPYRFGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLGMFLSRGYVKVRLGC
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEEEEEEC
CRGKKAYDKRRTIIEREKEREVAAAMKRRHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQKEIVSNRKALRNYEVIETLEAGIVLTGTEIKSLRDHGGNLGDAYVIVSKGEGWLLNA
CCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCCCCEEEEEECCCCEEEEC
SIAPYRFGNIYNHEERRKRKLLLHRYELRKLEGKIAQKGMTLIPLGMFLSRGYVKVRLGC
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCEEEEEEEC
CRGKKAYDKRRTIIEREKEREVAAAMKRRHH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362