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The map label for this gene is tmk

Identifier: 15835808

GI number: 15835808

Start: 304846

End: 305466

Strand: Reverse

Name: tmk

Synonym: CPj0273

Alternate gene names: 15835808

Gene position: 305466-304846 (Counterclockwise)

Preceding gene: 15835809

Following gene: 15835807

Centisome position: 24.9

GC content: 42.83

Gene sequence:

>621_bases
GTGTTTATCGTGATTGAGGGGGGCGAAGGGTCTGGCAAAAGTTCTTTAGCAAAGGCTTTAGGAGATCAGCTAGTAGCTCA
AGATCGGAAGGTTTTGTTAACAAGGGAACCTGGAGGGTGCCTTATAGGCGAAAGACTCCGAGATTTAATTTTGGAACCTC
CTCACTTAGAACTCTCTCGCTGTTGTGAACTCTTTTTGTTTCTTGGATCACGTGCTCAACATATTCAGGAAGTGATCATT
CCCGCATTGCGTGATGGCTATATTGTCATTTGTGAGAGATTCCACGATTCCACGATTGTGTATCAGGGAATAGCCGAAGG
TTTGGGTGCTGATTTTGTTGCCGACCTTTGTTCTAAGGTTGTAGGACCTACACCTTTCCTTCCTAATTTTGTTTTACTCT
TGGATATTCCTGCAGACATTGGCTTACAAAGGAAGCACCGACAAAAGGTTTTTGACAAGTTTGAAAAAAAGCCTTTGAGC
TATCATAACAGGATCCGAGAAGGATTTTTATCTCTTGCTAGTGCCGATCCTAGTCGTTACTTGGTTCTTGATGCTCGTGA
ATCACTAGCTAGTTTAATAGACAAAGTGATGCTACATACGCAACTTGGGTTATGCACCTAG

Upstream 100 bases:

>100_bases
CTTGTTTCTATGGAAAAACTCTCTTCTAATGAAAATGATGATGAAGTACTATCAGGGTCCGAAGAAGAATGTTCTGATAC
AGTAAGCCTAAGGTAGTATT

Downstream 100 bases:

>100_bases
AAGAAGAGAATCAAGGCTGGGAAGCTTTACTTAGGAAAGTTTATCATCAGGAAGTGCCTCCAGCTATACTGCTGCATGGA
TTTACCCTTCCTGTTTTACA

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MFIVIEGGEGSGKSSLAKALGDQLVAQDRKVLLTREPGGCLIGERLRDLILEPPHLELSRCCELFLFLGSRAQHIQEVII
PALRDGYIVICERFHDSTIVYQGIAEGLGADFVADLCSKVVGPTPFLPNFVLLLDIPADIGLQRKHRQKVFDKFEKKPLS
YHNRIREGFLSLASADPSRYLVLDARESLASLIDKVMLHTQLGLCT

Sequences:

>Translated_206_residues
MFIVIEGGEGSGKSSLAKALGDQLVAQDRKVLLTREPGGCLIGERLRDLILEPPHLELSRCCELFLFLGSRAQHIQEVII
PALRDGYIVICERFHDSTIVYQGIAEGLGADFVADLCSKVVGPTPFLPNFVLLLDIPADIGLQRKHRQKVFDKFEKKPLS
YHNRIREGFLSLASADPSRYLVLDARESLASLIDKVMLHTQLGLCT
>Mature_206_residues
MFIVIEGGEGSGKSSLAKALGDQLVAQDRKVLLTREPGGCLIGERLRDLILEPPHLELSRCCELFLFLGSRAQHIQEVII
PALRDGYIVICERFHDSTIVYQGIAEGLGADFVADLCSKVVGPTPFLPNFVLLLDIPADIGLQRKHRQKVFDKFEKKPLS
YHNRIREGFLSLASADPSRYLVLDARESLASLIDKVMLHTQLGLCT

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=199, Percent_Identity=33.6683417085427, Blast_Score=111, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_CHLPN (Q9Z8R5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86525
- PIR:   F72098
- RefSeq:   NP_224478.1
- RefSeq:   NP_300332.1
- RefSeq:   NP_445033.1
- RefSeq:   NP_876556.1
- ProteinModelPortal:   Q9Z8R5
- SMR:   Q9Z8R5
- GeneID:   1466963
- GeneID:   895180
- GeneID:   919056
- GeneID:   962851
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0486
- KEGG:   cpn:CPn0273
- KEGG:   cpt:CpB0280
- TIGR:   CP_0486
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- PhylomeDB:   Q9Z8R5
- ProtClustDB:   PRK00698
- BioCyc:   CPNE115711:CP_0486-MONOMER
- BioCyc:   CPNE115713:CPN0273-MONOMER
- BioCyc:   CPNE138677:CPJ0273-MONOMER
- BioCyc:   CPNE182082:CPB0280-MONOMER
- BRENDA:   2.7.4.9
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22853; Mature: 22853

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIVIEGGEGSGKSSLAKALGDQLVAQDRKVLLTREPGGCLIGERLRDLILEPPHLELSR
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHH
CCELFLFLGSRAQHIQEVIIPALRDGYIVICERFHDSTIVYQGIAEGLGADFVADLCSKV
HHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
VGPTPFLPNFVLLLDIPADIGLQRKHRQKVFDKFEKKPLSYHNRIREGFLSLASADPSRY
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEE
LVLDARESLASLIDKVMLHTQLGLCT
EEEECHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFIVIEGGEGSGKSSLAKALGDQLVAQDRKVLLTREPGGCLIGERLRDLILEPPHLELSR
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHH
CCELFLFLGSRAQHIQEVIIPALRDGYIVICERFHDSTIVYQGIAEGLGADFVADLCSKV
HHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
VGPTPFLPNFVLLLDIPADIGLQRKHRQKVFDKFEKKPLSYHNRIREGFLSLASADPSRY
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEE
LVLDARESLASLIDKVMLHTQLGLCT
EEEECHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362