Click here to switch to the map view.

The map label for this gene is gyrA_1

Identifier: 15835809

GI number: 15835809

Start: 305471

End: 307975

Strand: Reverse

Name: gyrA_1

Synonym: CPj0274

Alternate gene names: 15835809

Gene position: 307975-305471 (Counterclockwise)

Preceding gene: 15835810

Following gene: 15835808

Centisome position: 25.11

GC content: 41.0

Gene sequence:

>2505_bases
ATGTTCAATAAAGATGAAATTATAGTCCCTAAAAATCTTGAAGAAGAGATGAAGGAAAGTTACCTTCGTTATTCGATGTC
TGTGATTATATCTCGGGCTCTTCCAGATATCCGGGATGGACTCAAGCCTTCACAAAGAAGAGTTCTTTATGCCATGAAGC
AGCTAAGTCTTTCTCCAGGAGCTAAGCATCGTAAATGTGCAAAAATTTGTGGGGATACCTCTGGAGACTACCATCCACAT
GGTGAGAGCGTCATTTATCCTACGCTTGTCCGTATGGCTCAGAACTGGGCGATGCGTTATCCTTTGGTGGACGGACAAGG
AAACTTCGGCTCCATAGACGGAGATCCACCTGCTGCTATGCGATACACAGAAGCTCGTCTTACCCATAGTGCTATGTATC
TTATGGAAGACTTGGACAAAGATACTGTGGATATTGTTCCAAACTATGATGAAACAAAACACGAGCCTGTAGTTTTTCCT
TCTAAATTTCCGAATCTTCTTTGCAATGGTTCTTCAGGAATTGCTGTTGGGATGGCAACGAATATCCCCCCACACAATTT
AGGGGAACTTATAGAAGCTACACTTCTTCTGCTTGCAAATCCACAGGCTTCTGTGGATGAGATTTTACAAGTCATGCCAG
GCCCTGATTTCCCTACAGGAGGAATTATCTGCGGTTCTGAAGGAATTCGCTCCACGTATACTACAGGACGAGGAAAGATT
AAAGTTCGAGCTCGTCTGCATGTAGAAGAGAATGAAGATAAACATCGTGAGAGCATCATCATCACAGAGATGCCTTATAA
TGTGAATAAATCACGTTTGATTGAGCAAATTGCAAATCTTGTGAATGAAAAGACTCTCGCAGGCATTTCAGATGTTCGCG
ATGAGTCTGATAAAGATGGAATCCGTGTTGTCCTTGAAATCAAAAAAGGAGAATCTTCGGAAATCATTATCAATAGGCTC
TACAAGTTCACCGATGTTCAGGTAACTTTTGGGGCAAATATGCTTGCCCTGGATAAGAACTTACCTAGAACGATGAGTAT
TCATAGGATGATTTCCGCTTGGATACGCCATCGTAAAGAAGTCATCCGTCGTAGGACTCGTTATGAATTAAATAAAGCAG
AAACACGAGCTCATGTTTTAGAAGGATACCTTAAGGCTTTATCTTGCTTAGATGCATTAGTAAAAACGATCCGCGAGAGT
GGGAATAAAGAGCATGCTAAAGAACGGATTATTGAATCTTTTGGTTTTACCGAACCTCAAGCTCTTGCGATTTTAGAACT
GCGTTTATATCAACTTACTGGTTTAGAAGCTGAAAAGATTCAAAAAGAGTACGAAGAGTTATTAAATAAGATAGCTTATT
ATAAACAAGTTTTATCGGATGAAGGTTTAGTAAAAGATATCATCAGAAATGAGTTACAAGATCTTTTGAAGCATCATAAG
GTAGCTCGACGCACTACTATAGAATTCGATGCTGATGATATTCGTGATATTGAAGACATCATTACCAATGAGTCTGTTAT
TATCACGATATCCGGAGATGATTACGTAAAGAGAATGCCTGTAAAAGTGTTCAAAGAACAACGTCGTGGCGGGCATGGAG
TTACGGGATTTGATATGAAGAAGGGAGCAGGGTTCCTTAAAGCTGTATACTCTGCCTTTACTAAAGACTATCTCTTAATC
TTCACCAATTTTGGCCAATGTTATTGGTTGAAGGTATGGCAGCTCCCAGAAGGGGAGCGGAGGGCGAAAGGTAAGCCCAT
CATCAACTTCCTAGAGGGAATTCGTCCTGGTGAGGAGCTCGCAGCTATATTGAATATTAAGAATTTTGATAATGCCGGCT
TCTTATTTTTAGCCACCAAACGTGGTGTTGTGAAAAAAGTTTCCTTAGACGCCTTTAGCAACCCTAGGAAAAAGGGAATA
CGTGCTTTAGAGATAGATGAGGGAGACGAACTCATAGCAGCTTGTCACATTGTTAGTGATGAAGAGAAGGTAATGTTATT
TACTCATCTTGGTATGGCAGTTCGCTTCCCCCATGAAAAAGTTCGTCCTATGGGTAGAACTGCACGTGGGGTTCGCGGTG
TCTCTTTGAAAAATGAAGAAGATAAAGTTGTCAGTTGTCAAATTGTGACTGAGAACCAGTCGGTTTTAATTGTATGTGAT
CAAGGCTTTGGTAAGAGATCTTTAGTTGAAGATTTCCGCGAAACCAATCGTGGAGGCGTGGGCGTACGATCTATCCTTAT
CAATGAAAGAAACGGCAATGTCCTAGGAGCTATTCCTGTTACGGATCACGACAGTATTTTATTAATGTCGAGTCAGGGAC
AGGCAATTCGCATTAACATGCAAGATGTTAGAGTGATGGGAAGATCGACTCAAGGGGTGCGTTTAGTTCACTTGAAAGAA
GGGGATGCTCTTGTTTCTATGGAAAAACTCTCTTCTAATGAAAATGATGATGAAGTACTATCAGGGTCCGAAGAAGAATG
TTCTGATACAGTAAGCCTAAGGTAG

Upstream 100 bases:

>100_bases
GTTGATGGGGGAAGAAGTCCCTCCAAGAAGAGAATTCATAGAAAGTCATGCTTTGTCCATTAGGATAAATAATTTAGATA
TTTAGGAGTCCACAATCCCT

Downstream 100 bases:

>100_bases
TATTGTGTTTATCGTGATTGAGGGGGGCGAAGGGTCTGGCAAAAGTTCTTTAGCAAAGGCTTTAGGAGATCAGCTAGTAG
CTCAAGATCGGAAGGTTTTG

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 834; Mature: 834

Protein sequence:

>834_residues
MFNKDEIIVPKNLEEEMKESYLRYSMSVIISRALPDIRDGLKPSQRRVLYAMKQLSLSPGAKHRKCAKICGDTSGDYHPH
GESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAMRYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFP
SKFPNLLCNGSSGIAVGMATNIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKI
KVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDGIRVVLEIKKGESSEIIINRL
YKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKEVIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRES
GNKEHAKERIIESFGFTEPQALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELQDLLKHHK
VARRTTIEFDADDIRDIEDIITNESVIITISGDDYVKRMPVKVFKEQRRGGHGVTGFDMKKGAGFLKAVYSAFTKDYLLI
FTNFGQCYWLKVWQLPEGERRAKGKPIINFLEGIRPGEELAAILNIKNFDNAGFLFLATKRGVVKKVSLDAFSNPRKKGI
RALEIDEGDELIAACHIVSDEEKVMLFTHLGMAVRFPHEKVRPMGRTARGVRGVSLKNEEDKVVSCQIVTENQSVLIVCD
QGFGKRSLVEDFRETNRGGVGVRSILINERNGNVLGAIPVTDHDSILLMSSQGQAIRINMQDVRVMGRSTQGVRLVHLKE
GDALVSMEKLSSNENDDEVLSGSEEECSDTVSLR

Sequences:

>Translated_834_residues
MFNKDEIIVPKNLEEEMKESYLRYSMSVIISRALPDIRDGLKPSQRRVLYAMKQLSLSPGAKHRKCAKICGDTSGDYHPH
GESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAMRYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFP
SKFPNLLCNGSSGIAVGMATNIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKI
KVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDGIRVVLEIKKGESSEIIINRL
YKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKEVIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRES
GNKEHAKERIIESFGFTEPQALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELQDLLKHHK
VARRTTIEFDADDIRDIEDIITNESVIITISGDDYVKRMPVKVFKEQRRGGHGVTGFDMKKGAGFLKAVYSAFTKDYLLI
FTNFGQCYWLKVWQLPEGERRAKGKPIINFLEGIRPGEELAAILNIKNFDNAGFLFLATKRGVVKKVSLDAFSNPRKKGI
RALEIDEGDELIAACHIVSDEEKVMLFTHLGMAVRFPHEKVRPMGRTARGVRGVSLKNEEDKVVSCQIVTENQSVLIVCD
QGFGKRSLVEDFRETNRGGVGVRSILINERNGNVLGAIPVTDHDSILLMSSQGQAIRINMQDVRVMGRSTQGVRLVHLKE
GDALVSMEKLSSNENDDEVLSGSEEECSDTVSLR
>Mature_834_residues
MFNKDEIIVPKNLEEEMKESYLRYSMSVIISRALPDIRDGLKPSQRRVLYAMKQLSLSPGAKHRKCAKICGDTSGDYHPH
GESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAMRYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFP
SKFPNLLCNGSSGIAVGMATNIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKI
KVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDGIRVVLEIKKGESSEIIINRL
YKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKEVIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRES
GNKEHAKERIIESFGFTEPQALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELQDLLKHHK
VARRTTIEFDADDIRDIEDIITNESVIITISGDDYVKRMPVKVFKEQRRGGHGVTGFDMKKGAGFLKAVYSAFTKDYLLI
FTNFGQCYWLKVWQLPEGERRAKGKPIINFLEGIRPGEELAAILNIKNFDNAGFLFLATKRGVVKKVSLDAFSNPRKKGI
RALEIDEGDELIAACHIVSDEEKVMLFTHLGMAVRFPHEKVRPMGRTARGVRGVSLKNEEDKVVSCQIVTENQSVLIVCD
QGFGKRSLVEDFRETNRGGVGVRSILINERNGNVLGAIPVTDHDSILLMSSQGQAIRINMQDVRVMGRSTQGVRLVHLKE
GDALVSMEKLSSNENDDEVLSGSEEECSDTVSLR

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913408, Length=317, Percent_Identity=24.9211356466877, Blast_Score=76, Evalue=2e-13,
Organism=Homo sapiens, GI19913406, Length=316, Percent_Identity=24.6835443037975, Blast_Score=69, Evalue=3e-11,
Organism=Escherichia coli, GI1788562, Length=861, Percent_Identity=44.5993031358885, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=681, Percent_Identity=34.3612334801762, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI212645845, Length=445, Percent_Identity=23.1460674157303, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=343, Percent_Identity=26.2390670553936, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136538, Length=364, Percent_Identity=25.5494505494505, Blast_Score=74, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_CHLPN (Q9Z8R4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86525
- PIR:   F81571
- PIR:   G72098
- RefSeq:   NP_224479.1
- RefSeq:   NP_300333.1
- RefSeq:   NP_445032.1
- RefSeq:   NP_876557.1
- ProteinModelPortal:   Q9Z8R4
- GeneID:   1466964
- GeneID:   894973
- GeneID:   919058
- GeneID:   963490
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0485
- KEGG:   cpn:CPn0274
- KEGG:   cpt:CpB0281
- TIGR:   CP_0485
- HOGENOM:   HBG635183
- OMA:   ELGNDWN
- ProtClustDB:   PRK05560
- BioCyc:   CPNE115711:CP_0485-MONOMER
- BioCyc:   CPNE115713:CPN0274-MONOMER
- BioCyc:   CPNE138677:CPJ0274-MONOMER
- BioCyc:   CPNE182082:CPB0281-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- Gene3D:   G3DSA:1.10.268.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 93840; Mature: 93840

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: ACT_SITE 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKDEIIVPKNLEEEMKESYLRYSMSVIISRALPDIRDGLKPSQRRVLYAMKQLSLSPG
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCCC
AKHRKCAKICGDTSGDYHPHGESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAM
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCEECCCCCCCCCCCCCCHHH
RYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFPSKFPNLLCNGSSGIAVGMAT
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEECCCCCCCEEECCCCCEEEEEEC
NIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKI
CCCCCCHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCEE
KVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDG
EEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
IRVVLEIKKGESSEIIINRLYKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKE
EEEEEEEECCCCCHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHH
VIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRESGNKEHAKERIIESFGFTEPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCH
ALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELQDLLKHHK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VARRTTIEFDADDIRDIEDIITNESVIITISGDDYVKRMPVKVFKEQRRGGHGVTGFDMK
HHHHEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCC
KGAGFLKAVYSAFTKDYLLIFTNFGQCYWLKVWQLPEGERRAKGKPIINFLEGIRPGEEL
CCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCHHHCCCCHHHHHHHCCCCHHHH
AAILNIKNFDNAGFLFLATKRGVVKKVSLDAFSNPRKKGIRALEIDEGDELIAACHIVSD
HHHHEECCCCCCCEEEEEECCCCEEEEEHHHCCCCHHHCCEEEEECCCCCEEEEEEEECC
EEKVMLFTHLGMAVRFPHEKVRPMGRTARGVRGVSLKNEEDKVVSCQIVTENQSVLIVCD
CCCEEEEEECCCEEECCHHHHCCCCCHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEC
QGFGKRSLVEDFRETNRGGVGVRSILINERNGNVLGAIPVTDHDSILLMSSQGQAIRINM
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEEH
QDVRVMGRSTQGVRLVHLKEGDALVSMEKLSSNENDDEVLSGSEEECSDTVSLR
HHHHEECCCCCCEEEEEEECCCCEEEHHHHCCCCCCHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure
MFNKDEIIVPKNLEEEMKESYLRYSMSVIISRALPDIRDGLKPSQRRVLYAMKQLSLSPG
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCCC
AKHRKCAKICGDTSGDYHPHGESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAM
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCEECCCCCCCCCCCCCCHHH
RYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFPSKFPNLLCNGSSGIAVGMAT
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEECCCCCCCEEECCCCCEEEEEEC
NIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKI
CCCCCCHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCEE
KVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDG
EEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
IRVVLEIKKGESSEIIINRLYKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKE
EEEEEEEECCCCCHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHH
VIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRESGNKEHAKERIIESFGFTEPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCH
ALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELQDLLKHHK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VARRTTIEFDADDIRDIEDIITNESVIITISGDDYVKRMPVKVFKEQRRGGHGVTGFDMK
HHHHEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCC
KGAGFLKAVYSAFTKDYLLIFTNFGQCYWLKVWQLPEGERRAKGKPIINFLEGIRPGEEL
CCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCHHHCCCCHHHHHHHCCCCHHHH
AAILNIKNFDNAGFLFLATKRGVVKKVSLDAFSNPRKKGIRALEIDEGDELIAACHIVSD
HHHHEECCCCCCCEEEEEECCCCEEEEEHHHCCCCHHHCCEEEEECCCCCEEEEEEEECC
EEKVMLFTHLGMAVRFPHEKVRPMGRTARGVRGVSLKNEEDKVVSCQIVTENQSVLIVCD
CCCEEEEEECCCEEECCHHHHCCCCCHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEC
QGFGKRSLVEDFRETNRGGVGVRSILINERNGNVLGAIPVTDHDSILLMSSQGQAIRINM
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEEH
QDVRVMGRSTQGVRLVHLKEGDALVSMEKLSSNENDDEVLSGSEEECSDTVSLR
HHHHEECCCCCCEEEEEEECCCCEEEHHHHCCCCCCHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362