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Identifier: 15835786

GI number: 15835786

Start: 285682

End: 287184

Strand: Direct

Name: Not Available

Synonym: CPj0251

Alternate gene names: 15835786

Gene position: 285682-287184 (Clockwise)

Preceding gene: 15835780

Following gene: 15835796

Centisome position: 23.29

GC content: 37.59

Gene sequence:

>1503_bases
ATGAGTCCTTTTAAGAAAATAGTAAATCGCTTACTATGCTATATTTCTTTTCAAAAAGAATCAAGAACTCTCCCAATCAT
TATTAGAGAACCTAGGATGACAACAAAAAGTTTAGATTCTTTCAATTCAGTTATTTCCAAAAATAAAATTCATTTTATTA
GTTTGGGATGCTCTCGGAACCTTGTAGATAGCGAAGTCATGCTAGGCATTCTTCTTAAGGCAGGTTACGAGTCTACTAAT
GAAATTGAAGATGCTGACTATTTAATTTTAAATACCTGTGCGTTTTTAAAAAGTGCTAGAGATGAAGCTAAAGATTATCT
AGACCATCTAATTGATGTAAAAAAAGAGAACGCTAAAATTATTGTAACTGGATGCATGACTTCCAACCACAAAGATGAGC
TTAAACCCTGGATGTCACACATCCATTACCTACTAGGTTCTGGGGATGTTGAGAATATTCTTTCTGCTATTGAGTCTCGT
GAATCTGGAGAAAAAATCTCTGCAAAGAGTTACATTGAGATGGGAGAAGTTCCAAGACAGCTTTCCACACCAAAACACTA
TGCCTATTTAAAAGTTGCTGAGGGCTGTAGAAAACGTTGTGCTTTTTGTATTATTCCTTCCATTAAAGGAAAGCTCCGCA
GCAAACCTCTGGATCAAATTCTTAAAGAATTCCGCATCCTTGTAAACAAGAGTGTGAAAGAGATTATATTGATAGCTCAA
GACCTAGGAGATTATGGAAAGGATCTCTCTACAGACCGCAGTTCGCAGCTAGAATCACTATTACATGAGTTACTGAAAGA
GCCTGGTGATTATTGGCTGCGGATGTTGTATTTATATCCTGATGAAGTGAGTGATGGCATTATAGATCTTATGCAATCTA
ATCCCAAACTTCTTCCCTATGTAGATATTCCCTTACAGCACATTAACGACCGTATTTTAAAGCAAATGCGAAGAACGACT
TCTAGGGAGCAAATCCTAGGATTCCTAGAAAAATTACGTGCCAAGGTTCCTCAGGTCTATATCCGTTCTTCTGTTATTGT
GGGTTTCCCTGGTGAAACTCAGGAAGAATTCCAGGAGTTAGCTGATTTTATTGGTGAGGGTTGGATTGATAATCTCGGAA
TTTTCTTGTACTCTCAAGAAGCGAATACCCCGGCAGCAGAACTCCCTGACCAGATACCAGAAAAAGTTAAAGAATCGAGG
TTGAAAATTCTATCTCAAATTCAGAAACGCAATGTGGATAAACATAATCAGAAGCTCATTGGGGAAAAAATAGAAGCAGT
TATTGATAACTATCATCCTGAAACGAATCTTTTACTCACTGCAAGGTTCTATGGACAAGCTCCTGAAGTGGACCCTTGTA
TTATTGTAAATGAGGCGAAGCTTGTTTCTCATTTTGGAGAAAGATGCTTTATAGAAATCACAGGGACTGCTGGTTACGAC
CTTGTAGGGCGTGTTGTAAAAAAATCTCAGAACCAAGCTTTGCTAAAAACTAGCAAAGCTTAG

Upstream 100 bases:

>100_bases
AGTCGACCTGTTGTTTTTCTTTTGTTTTTTACAGTCCAGTACATATCAGAACTTTCCGAGCTTTTTAATTTAATAATCTC
CCGATTCTTGCTTGCCATGG

Downstream 100 bases:

>100_bases
GGTCCTGTGGTTAGTTACCAAAAGGCAAAGTCGCTATATTCCAAGAAAAACTTTGCACTCTAAAGCTATAACTTCGATTT
TCCAAAACAAAGTATCCAGG

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSPFKKIVNRLLCYISFQKESRTLPIIIREPRMTTKSLDSFNSVISKNKIHFISLGCSRNLVDSEVMLGILLKAGYESTN
EIEDADYLILNTCAFLKSARDEAKDYLDHLIDVKKENAKIIVTGCMTSNHKDELKPWMSHIHYLLGSGDVENILSAIESR
ESGEKISAKSYIEMGEVPRQLSTPKHYAYLKVAEGCRKRCAFCIIPSIKGKLRSKPLDQILKEFRILVNKSVKEIILIAQ
DLGDYGKDLSTDRSSQLESLLHELLKEPGDYWLRMLYLYPDEVSDGIIDLMQSNPKLLPYVDIPLQHINDRILKQMRRTT
SREQILGFLEKLRAKVPQVYIRSSVIVGFPGETQEEFQELADFIGEGWIDNLGIFLYSQEANTPAAELPDQIPEKVKESR
LKILSQIQKRNVDKHNQKLIGEKIEAVIDNYHPETNLLLTARFYGQAPEVDPCIIVNEAKLVSHFGERCFIEITGTAGYD
LVGRVVKKSQNQALLKTSKA

Sequences:

>Translated_500_residues
MSPFKKIVNRLLCYISFQKESRTLPIIIREPRMTTKSLDSFNSVISKNKIHFISLGCSRNLVDSEVMLGILLKAGYESTN
EIEDADYLILNTCAFLKSARDEAKDYLDHLIDVKKENAKIIVTGCMTSNHKDELKPWMSHIHYLLGSGDVENILSAIESR
ESGEKISAKSYIEMGEVPRQLSTPKHYAYLKVAEGCRKRCAFCIIPSIKGKLRSKPLDQILKEFRILVNKSVKEIILIAQ
DLGDYGKDLSTDRSSQLESLLHELLKEPGDYWLRMLYLYPDEVSDGIIDLMQSNPKLLPYVDIPLQHINDRILKQMRRTT
SREQILGFLEKLRAKVPQVYIRSSVIVGFPGETQEEFQELADFIGEGWIDNLGIFLYSQEANTPAAELPDQIPEKVKESR
LKILSQIQKRNVDKHNQKLIGEKIEAVIDNYHPETNLLLTARFYGQAPEVDPCIIVNEAKLVSHFGERCFIEITGTAGYD
LVGRVVKKSQNQALLKTSKA
>Mature_499_residues
SPFKKIVNRLLCYISFQKESRTLPIIIREPRMTTKSLDSFNSVISKNKIHFISLGCSRNLVDSEVMLGILLKAGYESTNE
IEDADYLILNTCAFLKSARDEAKDYLDHLIDVKKENAKIIVTGCMTSNHKDELKPWMSHIHYLLGSGDVENILSAIESRE
SGEKISAKSYIEMGEVPRQLSTPKHYAYLKVAEGCRKRCAFCIIPSIKGKLRSKPLDQILKEFRILVNKSVKEIILIAQD
LGDYGKDLSTDRSSQLESLLHELLKEPGDYWLRMLYLYPDEVSDGIIDLMQSNPKLLPYVDIPLQHINDRILKQMRRTTS
REQILGFLEKLRAKVPQVYIRSSVIVGFPGETQEEFQELADFIGEGWIDNLGIFLYSQEANTPAAELPDQIPEKVKESRL
KILSQIQKRNVDKHNQKLIGEKIEAVIDNYHPETNLLLTARFYGQAPEVDPCIIVNEAKLVSHFGERCFIEITGTAGYDL
VGRVVKKSQNQALLKTSKA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=23.7006237006237, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=23.7006237006237, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI93277076, Length=373, Percent_Identity=27.0777479892761, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=37.280701754386, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=27.5938189845475, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=404, Percent_Identity=24.2574257425743, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71996771, Length=310, Percent_Identity=26.7741935483871, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356207, Length=426, Percent_Identity=26.7605633802817, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI19922432, Length=382, Percent_Identity=26.4397905759162, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 56892; Mature: 56760

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFKKIVNRLLCYISFQKESRTLPIIIREPRMTTKSLDSFNSVISKNKIHFISLGCSRN
CCHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
LVDSEVMLGILLKAGYESTNEIEDADYLILNTCAFLKSARDEAKDYLDHLIDVKKENAKI
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IVTGCMTSNHKDELKPWMSHIHYLLGSGDVENILSAIESRESGEKISAKSYIEMGEVPRQ
EEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHH
LSTPKHYAYLKVAEGCRKRCAFCIIPSIKGKLRSKPLDQILKEFRILVNKSVKEIILIAQ
HCCCCHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
DLGDYGKDLSTDRSSQLESLLHELLKEPGDYWLRMLYLYPDEVSDGIIDLMQSNPKLLPY
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHEEHEECCCHHHHHHHHHHHCCCCEEEE
VDIPLQHINDRILKQMRRTTSREQILGFLEKLRAKVPQVYIRSSVIVGFPGETQEEFQEL
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECEEEEECCCCCHHHHHHH
ADFIGEGWIDNLGIFLYSQEANTPAAELPDQIPEKVKESRLKILSQIQKRNVDKHNQKLI
HHHHCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GEKIEAVIDNYHPETNLLLTARFYGQAPEVDPCIIVNEAKLVSHFGERCFIEITGTAGYD
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHCCCEEEEEEECCCCHH
LVGRVVKKSQNQALLKTSKA
HHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
SPFKKIVNRLLCYISFQKESRTLPIIIREPRMTTKSLDSFNSVISKNKIHFISLGCSRN
CHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
LVDSEVMLGILLKAGYESTNEIEDADYLILNTCAFLKSARDEAKDYLDHLIDVKKENAKI
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IVTGCMTSNHKDELKPWMSHIHYLLGSGDVENILSAIESRESGEKISAKSYIEMGEVPRQ
EEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHH
LSTPKHYAYLKVAEGCRKRCAFCIIPSIKGKLRSKPLDQILKEFRILVNKSVKEIILIAQ
HCCCCHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
DLGDYGKDLSTDRSSQLESLLHELLKEPGDYWLRMLYLYPDEVSDGIIDLMQSNPKLLPY
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHEEHEECCCHHHHHHHHHHHCCCCEEEE
VDIPLQHINDRILKQMRRTTSREQILGFLEKLRAKVPQVYIRSSVIVGFPGETQEEFQEL
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECEEEEECCCCCHHHHHHH
ADFIGEGWIDNLGIFLYSQEANTPAAELPDQIPEKVKESRLKILSQIQKRNVDKHNQKLI
HHHHCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GEKIEAVIDNYHPETNLLLTARFYGQAPEVDPCIIVNEAKLVSHFGERCFIEITGTAGYD
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHCCCEEEEEEECCCCHH
LVGRVVKKSQNQALLKTSKA
HHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10871362; 10684935 [H]