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The map label for this gene is tgt

Identifier: 15835754

GI number: 15835754

Start: 258979

End: 260097

Strand: Direct

Name: tgt

Synonym: CPj0219

Alternate gene names: 15835754

Gene position: 258979-260097 (Clockwise)

Preceding gene: 15835752

Following gene: 15835755

Centisome position: 21.11

GC content: 44.24

Gene sequence:

>1119_bases
TTGGCACTCAAATTCCATCTCATCCATCAATCTAAGAAATCCCAAGCTAGGGTCGGACAAATAGAGACCAGCCATGGAGT
GATCGATACACCCGCATTTGTCCCCGTAGCAACTCACGGAGCTTTAAAAGGAGTGATTGATCACAGCGATATTCCTCTGC
TCTTCTGTAATACCTACCACCTTCTTCTTCATCCAGGCCCAGAAGCAGTAGCTAAACTTGGGGGGCTGCACCAGTTTATG
GGACGTCAAGCACCAATCATTACAGATTCCGGGGGATTTCAAATTTTTAGCCTAGCCTATGGTTCTGTAGCTGAAGAAAT
CAAAAGTTGTGGCAAAAAAAAAGGCATGTCCTCTCTAGTTAAAATTACTGATGAAGGCGCATGGTTCAAATCCTATAGAG
ACGGGAGAAAGCTATTCCTCTCTCCAGAACTCTCAGTACAAGCCCAAAAAGATCTCGGAGCTGATATTATTATCCCTCTA
GACGAGCTTCTCCCCTTCCATACAGACCAAGAATACTTCTTAACTTCGTGTTCCCGTACGTATGTCTGGGAAAAACGTTC
TTTAGAATATCATCGAAAGGATCCTAGACACCAATCCATGTATGGGGTAATCCACGGAGGCCTCGATCCAGAACAACGTC
GTATTGGCGTTCGTTTTGTTGAGGATGAGCCATTCGATGGCTCTGCTATCGGAGGCAGCCTAGGAAGAAACCTTCAAGAA
ATGTCTGAAGTGGTTAAAATCACCACTTCATTTCTATCAAAAGAACGTCCCGTACACCTATTAGGAATCGGCGATCTTCC
CTCCATATACGCTATGGTCGGCTTTGGCATAGACTCTTTCGACAGTTCTTACCCGACTAAAGCTGCCCGTCATGGTCTTA
TCTTATCAAAAGCAGGACCCATCAAAATCGGTCAGCAAAAATATAGTCAGGACTCTTCCACTATAGACCCCTCGTGCTCT
TGTTTGACCTGCTTGTCAGGAATCTCTAGGGCATACCTGAGACACCTTTTTAAAGTAAGAGAACCTAACGCTGCTATCTG
GGCTTCTATACATAATCTACATCACATGCAACAAGTGATGAAAGAGATTCGTGAAGCCATCTTAAAAGATGAAATCTAA

Upstream 100 bases:

>100_bases
CAGATAAAACAAACCCTATTTACTTAAAAATTTTGTCATTGTAAACTTCCTCTTCCCTAAAAGACACATCCACCTTGTTC
TTTATAGTTAAGGATCTAGT

Downstream 100 bases:

>100_bases
GTCTCCTTTCTAAAGATTCCACATCTAAAAAACTCTAATTTTTCCATTTATTTTCAAAAAAATCTGTAAAGATTTCTTTA
TTTAGATCACAAAATACTCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MALKFHLIHQSKKSQARVGQIETSHGVIDTPAFVPVATHGALKGVIDHSDIPLLFCNTYHLLLHPGPEAVAKLGGLHQFM
GRQAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGMSSLVKITDEGAWFKSYRDGRKLFLSPELSVQAQKDLGADIIIPL
DELLPFHTDQEYFLTSCSRTYVWEKRSLEYHRKDPRHQSMYGVIHGGLDPEQRRIGVRFVEDEPFDGSAIGGSLGRNLQE
MSEVVKITTSFLSKERPVHLLGIGDLPSIYAMVGFGIDSFDSSYPTKAARHGLILSKAGPIKIGQQKYSQDSSTIDPSCS
CLTCLSGISRAYLRHLFKVREPNAAIWASIHNLHHMQQVMKEIREAILKDEI

Sequences:

>Translated_372_residues
MALKFHLIHQSKKSQARVGQIETSHGVIDTPAFVPVATHGALKGVIDHSDIPLLFCNTYHLLLHPGPEAVAKLGGLHQFM
GRQAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGMSSLVKITDEGAWFKSYRDGRKLFLSPELSVQAQKDLGADIIIPL
DELLPFHTDQEYFLTSCSRTYVWEKRSLEYHRKDPRHQSMYGVIHGGLDPEQRRIGVRFVEDEPFDGSAIGGSLGRNLQE
MSEVVKITTSFLSKERPVHLLGIGDLPSIYAMVGFGIDSFDSSYPTKAARHGLILSKAGPIKIGQQKYSQDSSTIDPSCS
CLTCLSGISRAYLRHLFKVREPNAAIWASIHNLHHMQQVMKEIREAILKDEI
>Mature_371_residues
ALKFHLIHQSKKSQARVGQIETSHGVIDTPAFVPVATHGALKGVIDHSDIPLLFCNTYHLLLHPGPEAVAKLGGLHQFMG
RQAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGMSSLVKITDEGAWFKSYRDGRKLFLSPELSVQAQKDLGADIIIPLD
ELLPFHTDQEYFLTSCSRTYVWEKRSLEYHRKDPRHQSMYGVIHGGLDPEQRRIGVRFVEDEPFDGSAIGGSLGRNLQEM
SEVVKITTSFLSKERPVHLLGIGDLPSIYAMVGFGIDSFDSSYPTKAARHGLILSKAGPIKIGQQKYSQDSSTIDPSCSC
LTCLSGISRAYLRHLFKVREPNAAIWASIHNLHHMQQVMKEIREAILKDEI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=370, Percent_Identity=33.7837837837838, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=35.1351351351351, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=32.6923076923077, Blast_Score=190, Evalue=9e-49,
Organism=Drosophila melanogaster, GI19920510, Length=374, Percent_Identity=35.0267379679144, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CHLPN (Q9Z8W5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72104
- PIR:   C86518
- RefSeq:   NP_224428.1
- RefSeq:   NP_300278.1
- RefSeq:   NP_445090.1
- RefSeq:   NP_876499.1
- ProteinModelPortal:   Q9Z8W5
- SMR:   Q9Z8W5
- GeneID:   1466906
- GeneID:   895042
- GeneID:   919013
- GeneID:   962725
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0546
- KEGG:   cpn:CPn0219
- KEGG:   cpt:CpB0223
- TIGR:   CP_0546
- HOGENOM:   HBG629929
- OMA:   SLHNIAH
- ProtClustDB:   PRK01008
- BioCyc:   CPNE115711:CP_0546-MONOMER
- BioCyc:   CPNE115713:CPN0219-MONOMER
- BioCyc:   CPNE138677:CPJ0219-MONOMER
- BioCyc:   CPNE182082:CPB0223-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41294; Mature: 41163

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKFHLIHQSKKSQARVGQIETSHGVIDTPAFVPVATHGALKGVIDHSDIPLLFCNTYH
CCEEEEEHHCCCHHHHHHCCEECCCCCCCCCCCCEEECCCHHHHCCCCCCCCEEEECCEE
LLLHPGPEAVAKLGGLHQFMGRQAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGMSSLV
EEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHH
KITDEGAWFKSYRDGRKLFLSPELSVQAQKDLGADIIIPLDELLPFHTDQEYFLTSCSRT
EECCCCCHHHHHCCCCEEEECCCCCEEECCCCCCEEEEEHHHHCCCCCCCHHHHHHCCHH
YVWEKRSLEYHRKDPRHQSMYGVIHGGLDPEQRRIGVRFVEDEPFDGSAIGGSLGRNLQE
EEEHHHCCHHHHCCCCHHHHHHHEECCCCHHHHEECEEEECCCCCCCCCCCCHHCCCHHH
MSEVVKITTSFLSKERPVHLLGIGDLPSIYAMVGFGIDSFDSSYPTKAARHGLILSKAGP
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCHHCCCCCCCHHHHCCEEEECCCC
IKIGQQKYSQDSSTIDPSCSCLTCLSGISRAYLRHLFKVREPNAAIWASIHNLHHMQQVM
CEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEIREAILKDEI
HHHHHHHHHCCC
>Mature Secondary Structure 
ALKFHLIHQSKKSQARVGQIETSHGVIDTPAFVPVATHGALKGVIDHSDIPLLFCNTYH
CEEEEEHHCCCHHHHHHCCEECCCCCCCCCCCCEEECCCHHHHCCCCCCCCEEEECCEE
LLLHPGPEAVAKLGGLHQFMGRQAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGMSSLV
EEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHH
KITDEGAWFKSYRDGRKLFLSPELSVQAQKDLGADIIIPLDELLPFHTDQEYFLTSCSRT
EECCCCCHHHHHCCCCEEEECCCCCEEECCCCCCEEEEEHHHHCCCCCCCHHHHHHCCHH
YVWEKRSLEYHRKDPRHQSMYGVIHGGLDPEQRRIGVRFVEDEPFDGSAIGGSLGRNLQE
EEEHHHCCHHHHCCCCHHHHHHHEECCCCHHHHEECEEEECCCCCCCCCCCCHHCCCHHH
MSEVVKITTSFLSKERPVHLLGIGDLPSIYAMVGFGIDSFDSSYPTKAARHGLILSKAGP
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCHHCCCCCCCHHHHCCEEEECCCC
IKIGQQKYSQDSSTIDPSCSCLTCLSGISRAYLRHLFKVREPNAAIWASIHNLHHMQQVM
CEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEIREAILKDEI
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362