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The map label for this gene is oppA_3

Identifier: 15835732

GI number: 15835732

Start: 237193

End: 238500

Strand: Direct

Name: oppA_3

Synonym: CPj0197

Alternate gene names: 15835732

Gene position: 237193-238500 (Clockwise)

Preceding gene: 15835731

Following gene: 15835733

Centisome position: 19.34

GC content: 41.44

Gene sequence:

>1308_bases
ATGTTTTCACGATGGATCACCCTCTTTTTATTATTCATTAGCCTTACTGGATGCTCCTCCTACTCTTCAAAACATAAACA
ATCTTTAATTATTCCCATACATGACGACCCTGTAGCTTTTTCTCCTGAACAAGCAAAACGGGCCATGGACCTTTCTATTG
CCCAACTTCTTTTTGATGGTCTGACTAGAGAAACTCATCGCGAATCCAATGATTTGGAATTAGCGATTGCCAGTCGCTAT
ACAGTCTCTGAAGACTTTTGCTCTTATACGTTCTTTATCAAAGACAGCGCTTTATGGAGCGACGGAACACCAATCACCTC
CGAAGATATCCGTAACGCTTGGGAGTATGCACAGGAGAACTCTCCCCACATACAGATCTTCCAAGGACTTAACTTCTCAA
CTCCTTCATCAAATGCAATTACGATTCATCTCGACTCGCCCAACCCCGATTTTCCTAAGCTTCTTGCCTTTCCTGCATTT
GCTATCTTTAAACCAGAAAACCCGAAGCTCTTTAGCGGTCCGTATACTCTTGTAGAGTATTTCCCAGGGCATAACATTCA
TTTAAAGAAAAACCCTAACTATTACGACTACCACTGCGTCTCCATCAACTCCATCAAACTGCTCATTATTCCTGATATAT
ATACAGCCATCCACCTCCTAAACAGAGGCAAGGTGGACTGGGTAGGACAACCCTGGCATCAAGGGATTCCTTGGGAGCTC
CATAAACAATCGCAATATCACTACTACACCTATCCTGTAGAAGGTGCCTTCTGGCTTTGTCTAAATACAAAATCCCCACA
CTTAAATGATCTTCAAAACAGACATAGACTCGCTACTTGTATTGATAAACGTTCTATCATTGAAGAAGCTCTTCAAGGAA
CCCAACAACCAGCGGAAACACTGTCCCGAGGAGCTCCACAACCAAATCAATATAAAAAACAAAAGCCTCTAACTCCACAA
GAAAAACTCGTGCTTACCTATCCCTCAGATATTCTAAGATGCCAACGCATAGCAGAAATCTTAAAGGAACAATGGAAAGC
TGCTGGAATAGATTTAATCCTTGAAGGACTCGAATACCATCTGTTTGTTAACAAACGAAAAGTCCAAGACTACGCCATAG
CAACACAGACTGGAGTTGCTTATTACCCAGGAGCAAATCTAATTTCTGAAGAAGACAAGCTCCTGCAAAACTTTGAGATT
ATCCCGATCTACTATCTGAGCTATGACTATCTCACTCAAGATTTTATAGAGGGAGTAATCTATAATGCTTCTGGAGCTGT
AGATCTCAAATATACCTATTTCCCCTAG

Upstream 100 bases:

>100_bases
TTTGTTCGACGAACTTCAGACTTTCGTTTTATAGAAAAACTATAGGAGAAAAAGTTTAGACTTAAAATTCGATAGTAAAT
TTATTAAAGTAATTTTTAAA

Downstream 100 bases:

>100_bases
ACAAAAGAAGTCTTTGGTAAGGGGTTTTTTTTATTGAAGAGACCTTTCTTCACCAGTATACTATTGTATCTTTATAAGAA
GTTTCTTCTGTATATAAATT

Product: oligopeptide binding protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFSRWITLFLLFISLTGCSSYSSKHKQSLIIPIHDDPVAFSPEQAKRAMDLSIAQLLFDGLTRETHRESNDLELAIASRY
TVSEDFCSYTFFIKDSALWSDGTPITSEDIRNAWEYAQENSPHIQIFQGLNFSTPSSNAITIHLDSPNPDFPKLLAFPAF
AIFKPENPKLFSGPYTLVEYFPGHNIHLKKNPNYYDYHCVSINSIKLLIIPDIYTAIHLLNRGKVDWVGQPWHQGIPWEL
HKQSQYHYYTYPVEGAFWLCLNTKSPHLNDLQNRHRLATCIDKRSIIEEALQGTQQPAETLSRGAPQPNQYKKQKPLTPQ
EKLVLTYPSDILRCQRIAEILKEQWKAAGIDLILEGLEYHLFVNKRKVQDYAIATQTGVAYYPGANLISEEDKLLQNFEI
IPIYYLSYDYLTQDFIEGVIYNASGAVDLKYTYFP

Sequences:

>Translated_435_residues
MFSRWITLFLLFISLTGCSSYSSKHKQSLIIPIHDDPVAFSPEQAKRAMDLSIAQLLFDGLTRETHRESNDLELAIASRY
TVSEDFCSYTFFIKDSALWSDGTPITSEDIRNAWEYAQENSPHIQIFQGLNFSTPSSNAITIHLDSPNPDFPKLLAFPAF
AIFKPENPKLFSGPYTLVEYFPGHNIHLKKNPNYYDYHCVSINSIKLLIIPDIYTAIHLLNRGKVDWVGQPWHQGIPWEL
HKQSQYHYYTYPVEGAFWLCLNTKSPHLNDLQNRHRLATCIDKRSIIEEALQGTQQPAETLSRGAPQPNQYKKQKPLTPQ
EKLVLTYPSDILRCQRIAEILKEQWKAAGIDLILEGLEYHLFVNKRKVQDYAIATQTGVAYYPGANLISEEDKLLQNFEI
IPIYYLSYDYLTQDFIEGVIYNASGAVDLKYTYFP
>Mature_435_residues
MFSRWITLFLLFISLTGCSSYSSKHKQSLIIPIHDDPVAFSPEQAKRAMDLSIAQLLFDGLTRETHRESNDLELAIASRY
TVSEDFCSYTFFIKDSALWSDGTPITSEDIRNAWEYAQENSPHIQIFQGLNFSTPSSNAITIHLDSPNPDFPKLLAFPAF
AIFKPENPKLFSGPYTLVEYFPGHNIHLKKNPNYYDYHCVSINSIKLLIIPDIYTAIHLLNRGKVDWVGQPWHQGIPWEL
HKQSQYHYYTYPVEGAFWLCLNTKSPHLNDLQNRHRLATCIDKRSIIEEALQGTQQPAETLSRGAPQPNQYKKQKPLTPQ
EKLVLTYPSDILRCQRIAEILKEQWKAAGIDLILEGLEYHLFVNKRKVQDYAIATQTGVAYYPGANLISEEDKLLQNFEI
IPIYYLSYDYLTQDFIEGVIYNASGAVDLKYTYFP

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI87081878, Length=436, Percent_Identity=24.0825688073394, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789397, Length=409, Percent_Identity=23.2273838630807, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787762, Length=311, Percent_Identity=22.1864951768489, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787495, Length=311, Percent_Identity=23.7942122186495, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789887, Length=365, Percent_Identity=26.8493150684932, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 50048; Mature: 50048

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRWITLFLLFISLTGCSSYSSKHKQSLIIPIHDDPVAFSPEQAKRAMDLSIAQLLFDG
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LTRETHRESNDLELAIASRYTVSEDFCSYTFFIKDSALWSDGTPITSEDIRNAWEYAQEN
HHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
SPHIQIFQGLNFSTPSSNAITIHLDSPNPDFPKLLAFPAFAIFKPENPKLFSGPYTLVEY
CCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHCCEEEEECCCCCCEECCCEEEEEE
FPGHNIHLKKNPNYYDYHCVSINSIKLLIIPDIYTAIHLLNRGKVDWVGQPWHQGIPWEL
CCCCEEEEECCCCEEEEEEEEECCEEEEEECCHHHHHHHHCCCCCCCCCCCHHCCCCEEE
HKQSQYHYYTYPVEGAFWLCLNTKSPHLNDLQNRHRLATCIDKRSIIEEALQGTQQPAET
ECCCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LSRGAPQPNQYKKQKPLTPQEKLVLTYPSDILRCQRIAEILKEQWKAAGIDLILEGLEYH
HHCCCCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEE
LFVNKRKVQDYAIATQTGVAYYPGANLISEEDKLLQNFEIIPIYYLSYDYLTQDFIEGVI
EEEECCCCCCEEEEECCCEEECCCCCCCCHHHHHHHCCCEEEEEEEEHHHHHHHHHHHEE
YNASGAVDLKYTYFP
ECCCCCEEEEEEECC
>Mature Secondary Structure
MFSRWITLFLLFISLTGCSSYSSKHKQSLIIPIHDDPVAFSPEQAKRAMDLSIAQLLFDG
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LTRETHRESNDLELAIASRYTVSEDFCSYTFFIKDSALWSDGTPITSEDIRNAWEYAQEN
HHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
SPHIQIFQGLNFSTPSSNAITIHLDSPNPDFPKLLAFPAFAIFKPENPKLFSGPYTLVEY
CCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHCCEEEEECCCCCCEECCCEEEEEE
FPGHNIHLKKNPNYYDYHCVSINSIKLLIIPDIYTAIHLLNRGKVDWVGQPWHQGIPWEL
CCCCEEEEECCCCEEEEEEEEECCEEEEEECCHHHHHHHHCCCCCCCCCCCHHCCCCEEE
HKQSQYHYYTYPVEGAFWLCLNTKSPHLNDLQNRHRLATCIDKRSIIEEALQGTQQPAET
ECCCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LSRGAPQPNQYKKQKPLTPQEKLVLTYPSDILRCQRIAEILKEQWKAAGIDLILEGLEYH
HHCCCCCCCHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEE
LFVNKRKVQDYAIATQTGVAYYPGANLISEEDKLLQNFEIIPIYYLSYDYLTQDFIEGVI
EEEECCCCCCEEEEECCCEEECCCCCCCCHHHHHHHCCCEEEEEEEEHHHHHHHHHHHEE
YNASGAVDLKYTYFP
ECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]