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The map label for this gene is oppA_4

Identifier: 15835733

GI number: 15835733

Start: 238784

End: 240364

Strand: Direct

Name: oppA_4

Synonym: CPj0198

Alternate gene names: 15835733

Gene position: 238784-240364 (Clockwise)

Preceding gene: 15835732

Following gene: 15835734

Centisome position: 19.47

GC content: 40.04

Gene sequence:

>1581_bases
ATGCATAGGCTTAAACCTACCTTAAAAAGTCTGATCCCTAATCTTCTTTTCTTATTGCTCACTCTTTCAAGCTGCTCAAA
GCAAAAACAAGAACCCTTAGGAAAACATCTCGTTATTGCGATGAGCCATGATCTCGCCGACCTAGATCCTCGCAATGCCT
ATTTAAGCAGAGATGCTTCCCTAGCAAAAGCCCTCTATGAAGGACTGACAAGAGAAACTGATCAAGGAATCGCACTGGCT
CTTGCAGAAAGTTATACCCTGTCAAAAGATCATAAGGTCTATACCTTTAAACTCAGACCTTCTGTGTGGAGCGATGGCAC
TCCACTCACTGCTTATGACTTTGAAAAATCTATAAAACAACTGTACTTCGAAGAATTTTCACCTTCCATACATACTTTAC
TCGGCGTGATTAAAAATTCTTCGGCAATCCACAATGCTCAAAAATCTCTGGAAACTCTTGGGATACAGGCAAAAGATGAT
CTTACTTTGGTGATTACCCTAGAGCAACCTTTCCCATACTTTCTCACACTTATCGCTCGCCCCGTATTCTCCCCTGTTCA
TCACACCCTTAGGGAATCCTATAAGAAAGGAACACCCCCATCCACATACATCTCCAATGGGCCCTTTGTCTTAAAAAAAC
ATGAACACCAAAACTACTTAATTTTAGAAAAAAATCCTCACTACTATGATCATGAATCAGTAAAGTTAGACCGAGTCACC
TTAAAAATTATCCCAGACGCCTCCACAGCCACGAAACTTTTCAAAAGTAAATCTATAGATTGGATTGGCTCACCTTGGAG
CGCTCCGATATCTAACGAAGACCAAAAAGTTCTCTCCCAAGAAAAGATTCTTACCTATTCTGTTTCAAGCACCACCCTTC
TTATCTATAACCTGCAAAAACCTCTAATACAAAATAAAGCCCTCAGGAAAGCCATTGCTCATGCTATTGATAGAAAATCT
ATCTTAAGACTCGTGCCTTCAGGACAAGAAGCTGTAACTCTAGTTCCCCCAAATCTTTCACAACTCAATCTTCAAAAAGA
GATCTCAACAGAAGAACGACAAACAAAAGCCAGAGCATATTTTCAAGAAGCTAAAGAAACACTTTCTGAAAAAGAACTCG
CAGAACTCAGCATCCTCTATCCTATAGATTCCTCGAATTCCTCCATCATAGCTCAAGAAATCCAAAGACAACTTAAAGAT
ACCTTAGGATTGAAAATCAAAATCCAAGGCATGGAGTACCACTGCTTTTTAAAGAAACGTCGTCAAGGAGATTTCTTCAT
AGCGACAGGAGGATGGATTGCGGAATACGTAAGCCCCGTAGCCTTCCTATCTATTCTAGGCAACCCCAGAGACCTCACAC
AATGGAGAAACAGTGATTACGAAAAGACTTTAGAGAAACTCTATCTCCCTCATGCCTACAAAGAGAATTTAAAACGCGCA
GAAATGATAATAGAAGAAGAAACCCCGATTATCCCCCTGTATCACGGCAAATATATTTACGCTATACATCCTAAAATCCA
GAATACATTCGGATCTCTTCTAGGCCACACAGATCTCAAAAATATCGATATCTTAAGTTAG

Upstream 100 bases:

>100_bases
ATTCCCCCATCGACTAAATCCACCACGGACTCCGACCTCCCATGTCTTCAATCCATATGAACGTAATATTAAGTAGCAAA
TTGAGTACTATATAATGAAG

Downstream 100 bases:

>100_bases
ATCCGAAATGGAAAAATTAAAAATTTTATAGACAATCTTGAAAAGAGAATTAAAAATTTTTAATTTAAATTATAGTTGCA
ATTGAAAACGCCCCTAAGAA

Product: oligopeptide binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MHRLKPTLKSLIPNLLFLLLTLSSCSKQKQEPLGKHLVIAMSHDLADLDPRNAYLSRDASLAKALYEGLTRETDQGIALA
LAESYTLSKDHKVYTFKLRPSVWSDGTPLTAYDFEKSIKQLYFEEFSPSIHTLLGVIKNSSAIHNAQKSLETLGIQAKDD
LTLVITLEQPFPYFLTLIARPVFSPVHHTLRESYKKGTPPSTYISNGPFVLKKHEHQNYLILEKNPHYYDHESVKLDRVT
LKIIPDASTATKLFKSKSIDWIGSPWSAPISNEDQKVLSQEKILTYSVSSTTLLIYNLQKPLIQNKALRKAIAHAIDRKS
ILRLVPSGQEAVTLVPPNLSQLNLQKEISTEERQTKARAYFQEAKETLSEKELAELSILYPIDSSNSSIIAQEIQRQLKD
TLGLKIKIQGMEYHCFLKKRRQGDFFIATGGWIAEYVSPVAFLSILGNPRDLTQWRNSDYEKTLEKLYLPHAYKENLKRA
EMIIEEETPIIPLYHGKYIYAIHPKIQNTFGSLLGHTDLKNIDILS

Sequences:

>Translated_526_residues
MHRLKPTLKSLIPNLLFLLLTLSSCSKQKQEPLGKHLVIAMSHDLADLDPRNAYLSRDASLAKALYEGLTRETDQGIALA
LAESYTLSKDHKVYTFKLRPSVWSDGTPLTAYDFEKSIKQLYFEEFSPSIHTLLGVIKNSSAIHNAQKSLETLGIQAKDD
LTLVITLEQPFPYFLTLIARPVFSPVHHTLRESYKKGTPPSTYISNGPFVLKKHEHQNYLILEKNPHYYDHESVKLDRVT
LKIIPDASTATKLFKSKSIDWIGSPWSAPISNEDQKVLSQEKILTYSVSSTTLLIYNLQKPLIQNKALRKAIAHAIDRKS
ILRLVPSGQEAVTLVPPNLSQLNLQKEISTEERQTKARAYFQEAKETLSEKELAELSILYPIDSSNSSIIAQEIQRQLKD
TLGLKIKIQGMEYHCFLKKRRQGDFFIATGGWIAEYVSPVAFLSILGNPRDLTQWRNSDYEKTLEKLYLPHAYKENLKRA
EMIIEEETPIIPLYHGKYIYAIHPKIQNTFGSLLGHTDLKNIDILS
>Mature_526_residues
MHRLKPTLKSLIPNLLFLLLTLSSCSKQKQEPLGKHLVIAMSHDLADLDPRNAYLSRDASLAKALYEGLTRETDQGIALA
LAESYTLSKDHKVYTFKLRPSVWSDGTPLTAYDFEKSIKQLYFEEFSPSIHTLLGVIKNSSAIHNAQKSLETLGIQAKDD
LTLVITLEQPFPYFLTLIARPVFSPVHHTLRESYKKGTPPSTYISNGPFVLKKHEHQNYLILEKNPHYYDHESVKLDRVT
LKIIPDASTATKLFKSKSIDWIGSPWSAPISNEDQKVLSQEKILTYSVSSTTLLIYNLQKPLIQNKALRKAIAHAIDRKS
ILRLVPSGQEAVTLVPPNLSQLNLQKEISTEERQTKARAYFQEAKETLSEKELAELSILYPIDSSNSSIIAQEIQRQLKD
TLGLKIKIQGMEYHCFLKKRRQGDFFIATGGWIAEYVSPVAFLSILGNPRDLTQWRNSDYEKTLEKLYLPHAYKENLKRA
EMIIEEETPIIPLYHGKYIYAIHPKIQNTFGSLLGHTDLKNIDILS

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI87081878, Length=501, Percent_Identity=28.3433133732535, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789397, Length=524, Percent_Identity=27.8625954198473, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=27.2373540856031, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787762, Length=545, Percent_Identity=24.2201834862385, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787052, Length=505, Percent_Identity=24.3564356435644, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789966, Length=484, Percent_Identity=21.0743801652893, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59824; Mature: 59824

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLKPTLKSLIPNLLFLLLTLSSCSKQKQEPLGKHLVIAMSHDLADLDPRNAYLSRDAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCHHH
LAKALYEGLTRETDQGIALALAESYTLSKDHKVYTFKLRPSVWSDGTPLTAYDFEKSIKQ
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LYFEEFSPSIHTLLGVIKNSSAIHNAQKSLETLGIQAKDDLTLVITLEQPFPYFLTLIAR
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHH
PVFSPVHHTLRESYKKGTPPSTYISNGPFVLKKHEHQNYLILEKNPHYYDHESVKLDRVT
HHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEE
LKIIPDASTATKLFKSKSIDWIGSPWSAPISNEDQKVLSQEKILTYSVSSTTLLIYNLQK
EEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCEEEEEECCC
PLIQNKALRKAIAHAIDRKSILRLVPSGQEAVTLVPPNLSQLNLQKEISTEERQTKARAY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCHHHHHCHHHHHHHHHHH
FQEAKETLSEKELAELSILYPIDSSNSSIIAQEIQRQLKDTLGLKIKIQGMEYHCFLKKR
HHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEHHC
RQGDFFIATGGWIAEYVSPVAFLSILGNPRDLTQWRNSDYEKTLEKLYLPHAYKENLKRA
CCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
EMIIEEETPIIPLYHGKYIYAIHPKIQNTFGSLLGHTDLKNIDILS
HHHEECCCCEEEEECCCEEEEECCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHRLKPTLKSLIPNLLFLLLTLSSCSKQKQEPLGKHLVIAMSHDLADLDPRNAYLSRDAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCHHH
LAKALYEGLTRETDQGIALALAESYTLSKDHKVYTFKLRPSVWSDGTPLTAYDFEKSIKQ
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
LYFEEFSPSIHTLLGVIKNSSAIHNAQKSLETLGIQAKDDLTLVITLEQPFPYFLTLIAR
HHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHH
PVFSPVHHTLRESYKKGTPPSTYISNGPFVLKKHEHQNYLILEKNPHYYDHESVKLDRVT
HHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEE
LKIIPDASTATKLFKSKSIDWIGSPWSAPISNEDQKVLSQEKILTYSVSSTTLLIYNLQK
EEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCEEEEEECCC
PLIQNKALRKAIAHAIDRKSILRLVPSGQEAVTLVPPNLSQLNLQKEISTEERQTKARAY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCHHHHHCHHHHHHHHHHH
FQEAKETLSEKELAELSILYPIDSSNSSIIAQEIQRQLKDTLGLKIKIQGMEYHCFLKKR
HHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEHHC
RQGDFFIATGGWIAEYVSPVAFLSILGNPRDLTQWRNSDYEKTLEKLYLPHAYKENLKRA
CCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
EMIIEEETPIIPLYHGKYIYAIHPKIQNTFGSLLGHTDLKNIDILS
HHHEECCCCEEEEECCCEEEEECCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]