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The map label for this gene is ptsN_2

Identifier: 15835597

GI number: 15835597

Start: 75210

End: 75887

Strand: Direct

Name: ptsN_2

Synonym: CPj0061

Alternate gene names: 15835597

Gene position: 75210-75887 (Clockwise)

Preceding gene: 15835596

Following gene: 15835598

Centisome position: 6.13

GC content: 39.09

Gene sequence:

>678_bases
ATGGATTTAAAGTTAGATGAAGTCGCCTCTTTGTTAGATGTTTCCGAACATACAGTTCTGCAATGGCTTAAAGAAGGAGC
CATTCCAAGCTATAGTATGAATAATGAATACCGCTTTAGTCGTGAAGAAATCGAAGACTGGCTATTGCATAACCAAGCAC
TCATGATCCAAGAACGCGGCGAAGATAAAGAAGCACTTAAAGATCTTTCTTTGAAATATAGTCTCTACAAAGCAATTCAT
CGTGGCGGCGTGCTTTGCGATGTTGTGGTTCATAGTAAAGAAGAAGCTCTCCAATACGCCTCTAAATACATCGCCCAAAA
GTTTCAATTAGACGAAAGCGTACTTTTTGAAATGCTCTCCCACAGAGAAAATCTTATGTCCACAGGTATAGGAGAAGGAA
TTGCCCTGCCCCATGCCAAAGACTTTTTAATTAATGCCTACTATGACATTGTGGTTCCTATGTTTCTTGCAGAGCCCATA
GAATACGGGGCTCTAGATGGAAAACCTGTAGGCATTCTTTTCTTCCTTTTTGCTTGCCAGGATAAAAGTCACTTAAACTT
AGTAAATAAAATAGTCCATCTCGGGATGTCTTTAAATGCCCGAAGCTTTTTTAAAAATTATCCTAACAAAGATCAACTTT
TAGCGTACGTTAAGGAATGGGAGTCCCAAACTCATTAA

Upstream 100 bases:

>100_bases
CTACTTTGACTTTATCTTTGAGAGAAGAGTCTCGTCGTCAACAGTTGTTACAGGTGAATACGATTGAAGAAGTCATGAAT
GTATTTGTGGGGATGTAAAA

Downstream 100 bases:

>100_bases
TAGCTAGAGTTTAAAAAGATTTTTAAGTCCAAGTTGTGAAAAAAATCCTTTTGTTGCTATGGTGATCCTCATAGGCTTCC
AGGAGATTGTAGTCGCATGA

Product: PTS system IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIA Component; Phosphotransferase IIA; Phosphotransferase System II; PTS System Fructose Subfamily IIA Subunit; PTS System IIA Protein; MerR Family Helix-Turn-Helix Transcriptional Regulator; PTS System Nitrogen Regulatory IIA Protein; Nitrogen Regulatory IIA Protein

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDLKLDEVASLLDVSEHTVLQWLKEGAIPSYSMNNEYRFSREEIEDWLLHNQALMIQERGEDKEALKDLSLKYSLYKAIH
RGGVLCDVVVHSKEEALQYASKYIAQKFQLDESVLFEMLSHRENLMSTGIGEGIALPHAKDFLINAYYDIVVPMFLAEPI
EYGALDGKPVGILFFLFACQDKSHLNLVNKIVHLGMSLNARSFFKNYPNKDQLLAYVKEWESQTH

Sequences:

>Translated_225_residues
MDLKLDEVASLLDVSEHTVLQWLKEGAIPSYSMNNEYRFSREEIEDWLLHNQALMIQERGEDKEALKDLSLKYSLYKAIH
RGGVLCDVVVHSKEEALQYASKYIAQKFQLDESVLFEMLSHRENLMSTGIGEGIALPHAKDFLINAYYDIVVPMFLAEPI
EYGALDGKPVGILFFLFACQDKSHLNLVNKIVHLGMSLNARSFFKNYPNKDQLLAYVKEWESQTH
>Mature_225_residues
MDLKLDEVASLLDVSEHTVLQWLKEGAIPSYSMNNEYRFSREEIEDWLLHNQALMIQERGEDKEALKDLSLKYSLYKAIH
RGGVLCDVVVHSKEEALQYASKYIAQKFQLDESVLFEMLSHRENLMSTGIGEGIALPHAKDFLINAYYDIVVPMFLAEPI
EYGALDGKPVGILFFLFACQDKSHLNLVNKIVHLGMSLNARSFFKNYPNKDQLLAYVKEWESQTH

Specific function: Seems To Have A Role In Linking Carbon And Nitrogen Assimilation. [C]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789597, Length=123, Percent_Identity=36.5853658536585, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 25861; Mature: 25861

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKLDEVASLLDVSEHTVLQWLKEGAIPSYSMNNEYRFSREEIEDWLLHNQALMIQERG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCHHHEEHHCC
EDKEALKDLSLKYSLYKAIHRGGVLCDVVVHSKEEALQYASKYIAQKFQLDESVLFEMLS
CHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HRENLMSTGIGEGIALPHAKDFLINAYYDIVVPMFLAEPIEYGALDGKPVGILFFLFACQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
DKSHLNLVNKIVHLGMSLNARSFFKNYPNKDQLLAYVKEWESQTH
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLKLDEVASLLDVSEHTVLQWLKEGAIPSYSMNNEYRFSREEIEDWLLHNQALMIQERG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCHHHEEHHCC
EDKEALKDLSLKYSLYKAIHRGGVLCDVVVHSKEEALQYASKYIAQKFQLDESVLFEMLS
CHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HRENLMSTGIGEGIALPHAKDFLINAYYDIVVPMFLAEPIEYGALDGKPVGILFFLFACQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
DKSHLNLVNKIVHLGMSLNARSFFKNYPNKDQLLAYVKEWESQTH
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA