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The map label for this gene is ptsN_1

Identifier: 15835596

GI number: 15835596

Start: 74731

End: 75207

Strand: Direct

Name: ptsN_1

Synonym: CPj0060

Alternate gene names: 15835596

Gene position: 74731-75207 (Clockwise)

Preceding gene: 15835595

Following gene: 15835597

Centisome position: 6.09

GC content: 41.51

Gene sequence:

>477_bases
ATGCCATCCTATTGTCAAAATCAACAAGATTTTTCTTTATTCTCTCTTTTGTCTCCTAGACTTGTAATGTTTTTAGGCAA
ACACTCCCGAGATGAAATCCTCCAAGATCTTACAGATCTTGTGGATGCTGCAGGCCTACTTGAAGACAAACAAGCCTTTT
TTGATGCTCTTGTCCGTCGTGAAAACATCATGTCCACAGGAATCGGAATGGGCGTGGCTATTCCTCACGGAAAACTCGAA
AGCTGCTCTAATTTTTTTATTGCTATAGGCATCCATACGCAAGGCATTTTATGGGACGCTATTGACGGAGCCCTCGTACG
CCTCGTCTTCTTGATCGGAGGTCCAGAAAATGCTCAAGCCGAATATCTCAAGTTATTATCTACTTTGACTTTATCTTTGA
GAGAAGAGTCTCGTCGTCAACAGTTGTTACAGGTGAATACGATTGAAGAAGTCATGAATGTATTTGTGGGGATGTAA

Upstream 100 bases:

>100_bases
TTATCTCCTGTAGTACAGGCAACGTTTGTTGTTAAGCAAGAAAGTTTAGCGGAAACTGCCCGAGGAAGTGGAGGTTTTGG
TCATACTGGAGCAAGCTAAG

Downstream 100 bases:

>100_bases
AAATGGATTTAAAGTTAGATGAAGTCGCCTCTTTGTTAGATGTTTCCGAACATACAGTTCTGCAATGGCTTAAAGAAGGA
GCCATTCCAAGCTATAGTAT

Product: Pts IIA protein

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MPSYCQNQQDFSLFSLLSPRLVMFLGKHSRDEILQDLTDLVDAAGLLEDKQAFFDALVRRENIMSTGIGMGVAIPHGKLE
SCSNFFIAIGIHTQGILWDAIDGALVRLVFLIGGPENAQAEYLKLLSTLTLSLREESRRQQLLQVNTIEEVMNVFVGM

Sequences:

>Translated_158_residues
MPSYCQNQQDFSLFSLLSPRLVMFLGKHSRDEILQDLTDLVDAAGLLEDKQAFFDALVRRENIMSTGIGMGVAIPHGKLE
SCSNFFIAIGIHTQGILWDAIDGALVRLVFLIGGPENAQAEYLKLLSTLTLSLREESRRQQLLQVNTIEEVMNVFVGM
>Mature_157_residues
PSYCQNQQDFSLFSLLSPRLVMFLGKHSRDEILQDLTDLVDAAGLLEDKQAFFDALVRRENIMSTGIGMGVAIPHGKLES
CSNFFIAIGIHTQGILWDAIDGALVRLVFLIGGPENAQAEYLKLLSTLTLSLREESRRQQLLQVNTIEEVMNVFVGM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=129, Percent_Identity=29.4573643410853, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI2367327, Length=115, Percent_Identity=31.304347826087, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789597, Length=109, Percent_Identity=34.8623853211009, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17616; Mature: 17484

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYCQNQQDFSLFSLLSPRLVMFLGKHSRDEILQDLTDLVDAAGLLEDKQAFFDALVRR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIMSTGIGMGVAIPHGKLESCSNFFIAIGIHTQGILWDAIDGALVRLVFLIGGPENAQA
HHHHHHCCCCCEECCCCCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHH
EYLKLLSTLTLSLREESRRQQLLQVNTIEEVMNVFVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSYCQNQQDFSLFSLLSPRLVMFLGKHSRDEILQDLTDLVDAAGLLEDKQAFFDALVRR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIMSTGIGMGVAIPHGKLESCSNFFIAIGIHTQGILWDAIDGALVRLVFLIGGPENAQA
HHHHHHCCCCCEECCCCCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHH
EYLKLLSTLTLSLREESRRQQLLQVNTIEEVMNVFVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]