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The map label for this gene is dut

Identifier: 15835595

GI number: 15835595

Start: 74292

End: 74729

Strand: Direct

Name: dut

Synonym: CPj0059

Alternate gene names: 15835595

Gene position: 74292-74729 (Clockwise)

Preceding gene: 15835594

Following gene: 15835596

Centisome position: 6.06

GC content: 45.66

Gene sequence:

>438_bases
ATGACTGTATTTTGTGAATTGGATTCAGGAGGAGAACTTCCTGAATATACTACGCCAGGAGCCGCTGGTGCGGATCTTAG
GGCAAACATCGAAGAACCCATCGCTCTGCTGCCTGGACAACGTGCTTTGATCCCTACCGGAATCAAAGCAGAAATTCCCG
AAGGGTACGAGCTACAGGTCCGTCCTCGGAGCGGTTTGGCTTTAAAGCACGGCATTACTGTTTTAAATTCCCCAGGGACT
ATCGATTCAGATTATAGGGGAGAGATTCGTGTAATCTTAATCAACTTCGGTGATAGTACATTCATTATTGAACCTAAGAT
GCGGATAGCTCAAGTTGTTTTATCTCCTGTAGTACAGGCAACGTTTGTTGTTAAGCAAGAAAGTTTAGCGGAAACTGCCC
GAGGAAGTGGAGGTTTTGGTCATACTGGAGCAAGCTAA

Upstream 100 bases:

>100_bases
TTTCGAAAAGGCTTAAAGAGATTTTTTTGTTGACAGATGACAGTGAATAAAACATCATACCGCATCTTGCAATGATAACA
TTATCTGTAACGCTATCCTT

Downstream 100 bases:

>100_bases
GATGCCATCCTATTGTCAAAATCAACAAGATTTTTCTTTATTCTCTCTTTTGTCTCCTAGACTTGTAATGTTTTTAGGCA
AACACTCCCGAGATGAAATC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTVFCELDSGGELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQVRPRSGLALKHGITVLNSPGT
IDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQATFVVKQESLAETARGSGGFGHTGAS

Sequences:

>Translated_145_residues
MTVFCELDSGGELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQVRPRSGLALKHGITVLNSPGT
IDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQATFVVKQESLAETARGSGGFGHTGAS
>Mature_144_residues
TVFCELDSGGELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQVRPRSGLALKHGITVLNSPGTI
DSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQATFVVKQESLAETARGSGGFGHTGAS

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=142, Percent_Identity=38.0281690140845, Blast_Score=86, Evalue=9e-18,
Organism=Homo sapiens, GI4503423, Length=142, Percent_Identity=38.0281690140845, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=38.5714285714286, Blast_Score=84, Evalue=6e-17,
Organism=Escherichia coli, GI1790071, Length=132, Percent_Identity=46.2121212121212, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71988561, Length=140, Percent_Identity=42.8571428571429, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=131, Percent_Identity=37.4045801526718, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583610, Length=141, Percent_Identity=35.4609929078014, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921126, Length=141, Percent_Identity=35.4609929078014, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CHLPN (Q9Z9C2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D72124
- PIR:   D86498
- RefSeq:   NP_224267.1
- RefSeq:   NP_300119.1
- RefSeq:   NP_445258.1
- RefSeq:   NP_876336.1
- ProteinModelPortal:   Q9Z9C2
- SMR:   Q9Z9C2
- GeneID:   1466743
- GeneID:   894712
- GeneID:   918848
- GeneID:   963183
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0716
- KEGG:   cpn:CPn0059
- KEGG:   cpt:CpB0060
- TIGR:   CP_0716
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q9Z9C2
- ProtClustDB:   PRK00601
- BioCyc:   CPNE115711:CP_0716-MONOMER
- BioCyc:   CPNE115713:CPN0059-MONOMER
- BioCyc:   CPNE138677:CPJ0059-MONOMER
- BioCyc:   CPNE182082:CPB0060-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15347; Mature: 15216

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: BINDING 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFCELDSGGELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCEEECCCCCEEEE
RPRSGLALKHGITVLNSPGTIDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQA
ECCCCEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHH
TFVVKQESLAETARGSGGFGHTGAS
HHEECHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TVFCELDSGGELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQV
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCEEECCCCCEEEE
RPRSGLALKHGITVLNSPGTIDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQA
ECCCCEEEECCEEEEECCCCCCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHH
TFVVKQESLAETARGSGGFGHTGAS
HHEECHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362