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The map label for this gene is hemC

Identifier: 15835588

GI number: 15835588

Start: 66977

End: 67681

Strand: Reverse

Name: hemC

Synonym: CPj0052

Alternate gene names: 15835588

Gene position: 67681-66977 (Counterclockwise)

Preceding gene: 15835589

Following gene: 15835586

Centisome position: 5.52

GC content: 45.39

Gene sequence:

>705_bases
ATGCTATCCGTCTGTTACTCTGACCCCTGTTTAAGTGATTTCTGTCAGGGCAAGCGCCCCCTTCGTATTGCTTCTAGAAA
TTCAAATTTAGCGAAAGCTCAAGTACATGAGTGTATTTCTTTGCTGCGCTCGTGGTATCCCAAACTTTGGTTCCAGTTAA
GCACTACAGAGACTACTGGGGATCGTGAGAAGAAAATCCCCTTACATCTTGTAGAAAACTCTTATTTTTTCACTGATGGC
GTCGATGCCTTAGTCCATAAAGGAGTCTGCGATCTGGCGATACACTCTGCTAAGGATCTTCCTGAGACTCCCTCTCTTCC
TGTAGTTGCCATAACACGATGTCTACATCCTGCAGACCTGTTAGTGTATGCTGACCACTATGTTCATGAGCCCCTACCCT
TGAGTCCCCGGCTAGGGAGTTCTTCTCTACGTCGAAGTGCAGTACTAAAACAACTGTTTCCTCAAGGACAGATCTTGGAT
ATTCGAGGCACTATTGAAGAGAGGCTGGATCAGCTCCACCGTGGTCATTATGATGCTATTGTTCTTGCGAAAGCTGCGAG
TTTAAGACTTCACCTGCATCATGCTTATAGTATAGAACTTCCTCCTCCATACCATGCCCTACAAGGATCCTTAGCAATTA
CAGCCAAGGATCACGCAGGTAAATGGAAACAGTTATTTACCCCTATACACTGCCACTCCTCATAA

Upstream 100 bases:

>100_bases
ATGGGATTTGAAGGTGCAATTCTTCCTGAGGGTCAAATTTCATCTCTTCCCAAGGAAATTCGAGAAAACTTTCGTTTACA
AGGAGTGAAAACAATAAAAG

Downstream 100 bases:

>100_bases
CTCTCCCCTCGCTTTTCATAGAATCGCAGGAGAGACTACCTGAATCTGGAGACACTATGTGGATAGAGGGTCCTCCTATA
TAGCTCTCATAAACCATCAA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLSVCYSDPCLSDFCQGKRPLRIASRNSNLAKAQVHECISLLRSWYPKLWFQLSTTETTGDREKKIPLHLVENSYFFTDG
VDALVHKGVCDLAIHSAKDLPETPSLPVVAITRCLHPADLLVYADHYVHEPLPLSPRLGSSSLRRSAVLKQLFPQGQILD
IRGTIEERLDQLHRGHYDAIVLAKAASLRLHLHHAYSIELPPPYHALQGSLAITAKDHAGKWKQLFTPIHCHSS

Sequences:

>Translated_234_residues
MLSVCYSDPCLSDFCQGKRPLRIASRNSNLAKAQVHECISLLRSWYPKLWFQLSTTETTGDREKKIPLHLVENSYFFTDG
VDALVHKGVCDLAIHSAKDLPETPSLPVVAITRCLHPADLLVYADHYVHEPLPLSPRLGSSSLRRSAVLKQLFPQGQILD
IRGTIEERLDQLHRGHYDAIVLAKAASLRLHLHHAYSIELPPPYHALQGSLAITAKDHAGKWKQLFTPIHCHSS
>Mature_234_residues
MLSVCYSDPCLSDFCQGKRPLRIASRNSNLAKAQVHECISLLRSWYPKLWFQLSTTETTGDREKKIPLHLVENSYFFTDG
VDALVHKGVCDLAIHSAKDLPETPSLPVVAITRCLHPADLLVYADHYVHEPLPLSPRLGSSSLRRSAVLKQLFPQGQILD
IRGTIEERLDQLHRGHYDAIVLAKAASLRLHLHHAYSIELPPPYHALQGSLAITAKDHAGKWKQLFTPIHCHSS

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=211, Percent_Identity=33.175355450237, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI66933009, Length=211, Percent_Identity=33.175355450237, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI48994974, Length=219, Percent_Identity=32.4200913242009, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319996, Length=227, Percent_Identity=30.3964757709251, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130425, Length=225, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CHLPN (Q9Z9C9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72126
- PIR:   E86497
- RefSeq:   NP_224260.1
- RefSeq:   NP_300112.1
- RefSeq:   NP_445265.1
- RefSeq:   NP_876329.1
- ProteinModelPortal:   Q9Z9C9
- SMR:   Q9Z9C9
- GeneID:   1466736
- GeneID:   895060
- GeneID:   918841
- GeneID:   962761
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0723
- KEGG:   cpn:CPn0052
- KEGG:   cpt:CpB0053
- TIGR:   CP_0723
- HOGENOM:   HBG414030
- OMA:   GQILDIR
- ProtClustDB:   PRK01066
- BioCyc:   CPNE115711:CP_0723-MONOMER
- BioCyc:   CPNE115713:CPN0052-MONOMER
- BioCyc:   CPNE138677:CPJ0052-MONOMER
- BioCyc:   CPNE182082:CPB0053-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022417
- PANTHER:   PTHR11557
- PRINTS:   PR00151

Pfam domain/function: PF01379 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 26245; Mature: 26245

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVCYSDPCLSDFCQGKRPLRIASRNSNLAKAQVHECISLLRSWYPKLWFQLSTTETTG
CCEEECCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DREKKIPLHLVENSYFFTDGVDALVHKGVCDLAIHSAKDLPETPSLPVVAITRCLHPADL
CCCCCCCEEEECCCEEEECCHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHH
LVYADHYVHEPLPLSPRLGSSSLRRSAVLKQLFPQGQILDIRGTIEERLDQLHRGHYDAI
EEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEE
VLAKAASLRLHLHHAYSIELPPPYHALQGSLAITAKDHAGKWKQLFTPIHCHSS
EEEECCEEEEEEEEEEEEECCCCHHHHCCCEEEEECCCCCCHHHHCCCEECCCC
>Mature Secondary Structure
MLSVCYSDPCLSDFCQGKRPLRIASRNSNLAKAQVHECISLLRSWYPKLWFQLSTTETTG
CCEEECCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DREKKIPLHLVENSYFFTDGVDALVHKGVCDLAIHSAKDLPETPSLPVVAITRCLHPADL
CCCCCCCEEEECCCEEEECCHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHH
LVYADHYVHEPLPLSPRLGSSSLRRSAVLKQLFPQGQILDIRGTIEERLDQLHRGHYDAI
EEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEE
VLAKAASLRLHLHHAYSIELPPPYHALQGSLAITAKDHAGKWKQLFTPIHCHSS
EEEECCEEEEEEEEEEEEECCCCHHHHCCCEEEEECCCCCCHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362