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The map label for this gene is pgk

Identifier: 16752362

GI number: 16752362

Start: 78654

End: 79862

Strand: Direct

Name: pgk

Synonym: CP0068

Alternate gene names: 16752362

Gene position: 78654-79862 (Clockwise)

Preceding gene: 16752361

Following gene: 16752363

Centisome position: 6.4

GC content: 44.33

Gene sequence:

>1209_bases
ATGGATAAGCTAACAGTACAAGATCTTTCTCCAGAAGAAAAAAAAGTCCTCGTACGTGTAGATTTCAATGTCCCCATGCA
AGATGGCAAGATACTCGATGACATTCGTATTCGCAGTGCGATGCCTACAATCAACTATCTACTTAAGAAACATGCTGCAG
TGATTTTAATGAGTCATTTAGGGCGACCTAAAGGACAGGGGTTCCAAGAGGAATATTCTCTGCAACCCGTTGTGGATGTT
CTCGAAGGATACTTAGGACATCATGTGCCTCTAGCTCCAGATTGTGTGGGTGAGGTTGCACGTCAAGCTGTAGCTCAGCT
TTCTCCTGGTCGCGTTTTGCTTCTTGAGAACTTGCGTTTCCATATAGGAGAAGAACATCCAGAAAAAGACCCGACGTTCG
CCGCAGAACTCTCTTCCTACGGGGATTTCTATGTCAACGATGCTTTCGGAACTTCGCATAGAAAACATGCTTCAGTCTAT
GTAGTGCCGCAGGCTTTCCCAGGTAGAGCCGCAGCAGGCCTGCTTATGGAAAAAGAATTGGAATTTTTAGGAAGACATCT
ATTGACCTCCCCTAAGAGGCCTTTCACTGCCATCCTTGGAGGAGCTAAGATTTCTTCTAAAATTGGAGTTATAGAGGCTC
TACTGAATCAAGTAGACTACCTCTTATTAGCTGGAGGTATGGGATTTACTTTCCTACAAGCCTTGGGAAAATCCCTAGGG
AACTCTCTTGTGGAGAAATCTGCCTTGGATCTTGCTAGAAATGTATTGAAAATTGCTAAAAGTCGTAATGTTACCATAGT
TTTGCCTAGCGATGTGAAAGCAGCCGAAAATCTCCAATCTAAGGAATATTCTGTGATTTCTATAGATCAAGGCATTCCTC
CGCATCTTCAAGGCTTTGATATTGGACCTAGAACAACCGAAGAATTTATCCGTATTATAAACCAATCAGCGACTGTGTTT
TGGAATGGTCCTGTGGGTGTTTATGAGGTCCCTCCTTTTGACTCTGGATCTATTGCTATAGCGAATGCCTTAGGCAATCA
CCCTTCAGCTGTTACTGTCGTGGGTGGAGGAGATGCGGCAGCTGTGGTTGCTTTGGCAGGCTGCTCTACAAAAGTTTCCC
ATGTTTCTACTGGAGGAGGGGCTTCTTTAGAGTTTCTAGAGCAGGGCTTCCTCCCAGGTACAGAAGTTTTATCTCCATCC
AAAAGCTAA

Upstream 100 bases:

>100_bases
CTTCCTGCAGGAGCTTTATTATCCATACTCTTTTTCTTTGCTTTAAGAGCTTTGTTCCATTAAAAATAAGAAAAATCAAA
TAGTAGGTTCTTGAGGATAT

Downstream 100 bases:

>100_bases
AGTACTCTTAAATCAACATACTTAAGAATTCGTGTTAAGAGATGTATCTTGTTGTCGTTTAGGTAAACACGATGCATCGC
GCTCTTCTTCTCTCTTAACA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MDKLTVQDLSPEEKKVLVRVDFNVPMQDGKILDDIRIRSAMPTINYLLKKHAAVILMSHLGRPKGQGFQEEYSLQPVVDV
LEGYLGHHVPLAPDCVGEVARQAVAQLSPGRVLLLENLRFHIGEEHPEKDPTFAAELSSYGDFYVNDAFGTSHRKHASVY
VVPQAFPGRAAAGLLMEKELEFLGRHLLTSPKRPFTAILGGAKISSKIGVIEALLNQVDYLLLAGGMGFTFLQALGKSLG
NSLVEKSALDLARNVLKIAKSRNVTIVLPSDVKAAENLQSKEYSVISIDQGIPPHLQGFDIGPRTTEEFIRIINQSATVF
WNGPVGVYEVPPFDSGSIAIANALGNHPSAVTVVGGGDAAAVVALAGCSTKVSHVSTGGGASLEFLEQGFLPGTEVLSPS
KS

Sequences:

>Translated_402_residues
MDKLTVQDLSPEEKKVLVRVDFNVPMQDGKILDDIRIRSAMPTINYLLKKHAAVILMSHLGRPKGQGFQEEYSLQPVVDV
LEGYLGHHVPLAPDCVGEVARQAVAQLSPGRVLLLENLRFHIGEEHPEKDPTFAAELSSYGDFYVNDAFGTSHRKHASVY
VVPQAFPGRAAAGLLMEKELEFLGRHLLTSPKRPFTAILGGAKISSKIGVIEALLNQVDYLLLAGGMGFTFLQALGKSLG
NSLVEKSALDLARNVLKIAKSRNVTIVLPSDVKAAENLQSKEYSVISIDQGIPPHLQGFDIGPRTTEEFIRIINQSATVF
WNGPVGVYEVPPFDSGSIAIANALGNHPSAVTVVGGGDAAAVVALAGCSTKVSHVSTGGGASLEFLEQGFLPGTEVLSPS
KS
>Mature_402_residues
MDKLTVQDLSPEEKKVLVRVDFNVPMQDGKILDDIRIRSAMPTINYLLKKHAAVILMSHLGRPKGQGFQEEYSLQPVVDV
LEGYLGHHVPLAPDCVGEVARQAVAQLSPGRVLLLENLRFHIGEEHPEKDPTFAAELSSYGDFYVNDAFGTSHRKHASVY
VVPQAFPGRAAAGLLMEKELEFLGRHLLTSPKRPFTAILGGAKISSKIGVIEALLNQVDYLLLAGGMGFTFLQALGKSLG
NSLVEKSALDLARNVLKIAKSRNVTIVLPSDVKAAENLQSKEYSVISIDQGIPPHLQGFDIGPRTTEEFIRIINQSATVF
WNGPVGVYEVPPFDSGSIAIANALGNHPSAVTVVGGGDAAAVVALAGCSTKVSHVSTGGGASLEFLEQGFLPGTEVLSPS
KS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=420, Percent_Identity=41.9047619047619, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=42.2062350119904, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=38.0352644836272, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17508823, Length=422, Percent_Identity=41.2322274881517, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6319857, Length=418, Percent_Identity=42.822966507177, Blast_Score=313, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=42.6506024096386, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI17136394, Length=421, Percent_Identity=41.8052256532066, Blast_Score=294, Evalue=9e-80,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CHLPN (Q9Z7M5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C72049
- PIR:   D86575
- RefSeq:   NP_224875.1
- RefSeq:   NP_300735.1
- RefSeq:   NP_444620.1
- RefSeq:   NP_876978.1
- ProteinModelPortal:   Q9Z7M5
- SMR:   Q9Z7M5
- PHCI-2DPAGE:   Q9Z7M5
- GeneID:   1467385
- GeneID:   895421
- GeneID:   919438
- GeneID:   963723
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0068
- KEGG:   cpn:CPn0679
- KEGG:   cpt:CpB0706
- TIGR:   CP_0068
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q9Z7M5
- ProtClustDB:   PRK00073
- BioCyc:   CPNE115711:CP_0068-MONOMER
- BioCyc:   CPNE115713:CPN0679-MONOMER
- BioCyc:   CPNE138677:CPJ0679-MONOMER
- BioCyc:   CPNE182082:CPB0706-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43047; Mature: 43047

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 154-154 BINDING 207-207 BINDING 298-298 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLTVQDLSPEEKKVLVRVDFNVPMQDGKILDDIRIRSAMPTINYLLKKHAAVILMSHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPKGQGFQEEYSLQPVVDVLEGYLGHHVPLAPDCVGEVARQAVAQLSPGRVLLLENLRF
CCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHH
HIGEEHPEKDPTFAAELSSYGDFYVNDAFGTSHRKHASVYVVPQAFPGRAAAGLLMEKEL
HCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
EFLGRHLLTSPKRPFTAILGGAKISSKIGVIEALLNQVDYLLLAGGMGFTFLQALGKSLG
HHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHH
NSLVEKSALDLARNVLKIAKSRNVTIVLPSDVKAAENLQSKEYSVISIDQGIPPHLQGFD
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCC
IGPRTTEEFIRIINQSATVFWNGPVGVYEVPPFDSGSIAIANALGNHPSAVTVVGGGDAA
CCCCCHHHHHHHHCCCCEEEECCCCCEEECCCCCCCCEEEEHHHCCCCCEEEEEECCCCE
AVVALAGCSTKVSHVSTGGGASLEFLEQGFLPGTEVLSPSKS
EHHEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDKLTVQDLSPEEKKVLVRVDFNVPMQDGKILDDIRIRSAMPTINYLLKKHAAVILMSHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPKGQGFQEEYSLQPVVDVLEGYLGHHVPLAPDCVGEVARQAVAQLSPGRVLLLENLRF
CCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHH
HIGEEHPEKDPTFAAELSSYGDFYVNDAFGTSHRKHASVYVVPQAFPGRAAAGLLMEKEL
HCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
EFLGRHLLTSPKRPFTAILGGAKISSKIGVIEALLNQVDYLLLAGGMGFTFLQALGKSLG
HHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHH
NSLVEKSALDLARNVLKIAKSRNVTIVLPSDVKAAENLQSKEYSVISIDQGIPPHLQGFD
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEECCCCCCCCCCCC
IGPRTTEEFIRIINQSATVFWNGPVGVYEVPPFDSGSIAIANALGNHPSAVTVVGGGDAA
CCCCCHHHHHHHHCCCCEEEECCCCCEEECCCCCCCCEEEEHHHCCCCCEEEEEECCCCE
AVVALAGCSTKVSHVSTGGGASLEFLEQGFLPGTEVLSPSKS
EHHEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362