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The map label for this gene is folD

Identifier: 13357897

GI number: 13357897

Start: 382856

End: 383665

Strand: Direct

Name: folD

Synonym: UU337

Alternate gene names: 13357897

Gene position: 382856-383665 (Clockwise)

Preceding gene: 13357896

Following gene: 13357898

Centisome position: 50.93

GC content: 20.49

Gene sequence:

>810_bases
ATGAAACTAAAAATTGATTTTTTATACGAAAAAGTCTTAACAGAATTATTAACTAAAAAAAACAAAACTAAAAAACGTAT
TTATGTTTTTTATGATTCAGATTTAGTAAATTTTAATAGTGCATATATTCAATCAAAATTAAAATATGCTAAATTATTTT
CTGTTAAACTTATTATTTATGATTTAAATGCATACCAAATTCAAGAAAATCATCATTTTTTATCAAAACTTAAACAAGCA
AATAAGGATTTTTTGTTTTTTGAATTCCCTTTGTCAAAAAAACATTTGGATCAAAATTATTTTCAATATTTAACTTTTCA
AAATGATCTCGATGGAATTAATTCACATTTGTTTTTAAATAAAAAACAAGAAAATCAAATTATTTACCATCCAGCAACAA
TTAATGCAATTTTAACATTAATTAAAGAATTAGATGTAAATTTAAATGACTATCGAATTTGCATTATTGGTCGTTCACCC
TATTTAGGGGATTATTTATATAAGTTATTAAATCCTAAAAATAATAAAATTAATATTATTCATTCACAAACTAAAAATCC
ATTAAGAATTATTAAAGAATCTAATTTTATTATTAGCTGTGTGAATAAAGCACACATTTTTAATTCTAATGCATTAAGTG
ATAATTCTTATTTAATTGATGTTACTACAGAATACATCAATAATAAGTTATGTGGTTCTTTTTTAATTAATAAAAATGAT
GCATTAAATCGAAACATTAAATACACTCCTGTACCAGGTGGAATCGGTAAATTAACGACGTTATTTTTATTTTTGAATTA
TTTTAAATAA

Upstream 100 bases:

>100_bases
TGTTAAGGTTTACGTTAACGATCAAGAAATTCTTTCTAAATAATTATTTTAAAGGAAACACAAAAAAGGCTTTTAATAAT
AAAAGCCTTTTTATTTCAAA

Downstream 100 bases:

>100_bases
GTGTTACTTATCAGCCATATTTTTTTGTAGTATTATATAATAATATAAATAATCAAATAAGAAAATAGTATTTATTATGA
TTGACAAGCAAAAAATTAAA

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIYDLNAYQIQENHHFLSKLKQA
NKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLNKKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSP
YLGDYLYKLLNPKNNKINIIHSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKND
ALNRNIKYTPVPGGIGKLTTLFLFLNYFK

Sequences:

>Translated_269_residues
MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIYDLNAYQIQENHHFLSKLKQA
NKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLNKKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSP
YLGDYLYKLLNPKNNKINIIHSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKND
ALNRNIKYTPVPGGIGKLTTLFLFLNYFK
>Mature_269_residues
MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIYDLNAYQIQENHHFLSKLKQA
NKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLNKKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSP
YLGDYLYKLLNPKNNKINIIHSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKND
ALNRNIKYTPVPGGIGKLTTLFLFLNYFK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319558, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321643, Length=250, Percent_Identity=24.8, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31609; Mature: 31609

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIY
CEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHEEEEEEEE
DLNAYQIQENHHFLSKLKQANKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLN
ECCEEEEECCHHHHHHHHHCCCCEEEEECCCCHHHCCCHHHHEEEECCCCCCCCCEEEEE
KKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSPYLGDYLYKLLNPKNNKINII
CCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEE
HSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKND
ECCCCCCEEEEECCCEEEEEECHHEEECCCCCCCCCEEEEEEHHHCCCCCCHHEEECCCC
ALNRNIKYTPVPGGIGKLTTLFLFLNYFK
CCCCCEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIY
CEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHEEEEEEEE
DLNAYQIQENHHFLSKLKQANKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLN
ECCEEEEECCHHHHHHHHHCCCCEEEEECCCCHHHCCCHHHHEEEECCCCCCCCCEEEEE
KKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSPYLGDYLYKLLNPKNNKINII
CCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEE
HSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKND
ECCCCCCEEEEECCCEEEEEECHHEEECCCCCCCCCEEEEEEHHHCCCCCCHHEEECCCC
ALNRNIKYTPVPGGIGKLTTLFLFLNYFK
CCCCCEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]