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The map label for this gene is greA

Identifier: 13357864

GI number: 13357864

Start: 348924

End: 349394

Strand: Direct

Name: greA

Synonym: UU304

Alternate gene names: 13357864

Gene position: 348924-349394 (Clockwise)

Preceding gene: 13357863

Following gene: 13357865

Centisome position: 46.42

GC content: 28.66

Gene sequence:

>471_bases
ATGGCAAAGTATACGATATCAAAACACCGTCTTGAAGAATTACAATTAGAATTAAGAGAAATTTTAGATGTTAAGTGACC
AGCAATTACAAAGCAATTACAAGATGCACGCGAACAAGGTGATTTATCTGAAAATGCTGATTATGATGCTGCAAAAAATG
AACAAGCAGCATTAAAAAAACGTAAAGATGAAATTGAAGAAATTTTAGAAAATTATGAATTAATTGAAGATGTTTTACGT
TCAACTGACGAAGTTTCCATTGGATCGACAATTGAAATTTATAATTATCAAAAAGATTGTGAAGAAGTAATTACACTAGT
TGGTTCAATGGATTCAGATCCTTTTGCAAACAAAATTTCAATAGACACACCACTTGGAAAAGCTCTTGTAAAACAAAAAA
AAGGTACTGAAGTTACAGTTCATACTTTAGCATCACCTTATAAAGTAAAAATTATAAAAATTATTGATTAA

Upstream 100 bases:

>100_bases
TTGAAATTTAAGAGGTTGGTTTTTTTATTTATTATTAATTTTTATATATTATGTAATGATATAATTAAATAATATATTTT
TATTTATATGGGGCAACACT

Downstream 100 bases:

>100_bases
TTTAACTAAAAAAAGATTGACATTATATAATAATTTTACTATAATTTTATAGTGTTTTATTAAACACACTATATAAATTG
GACAGATAGCGAAGTGGCCA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAKYTISKHRLEELQLELREILDVKWPAITKQLQDAREQGDLSENADYDAAKNEQAALKKRKDEIEEILENYELIEDVLR
STDEVSIGSTIEIYNYQKDCEEVITLVGSMDSDPFANKISIDTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID

Sequences:

>Translated_156_residues
MAKYTISKHRLEELQLELREILDVK*PAITKQLQDAREQGDLSENADYDAAKNEQAALKKRKDEIEEILENYELIEDVLR
STDEVSIGSTIEIYNYQKDCEEVITLVGSMDSDPFANKISIDTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID
>Mature_155_residues
AKYTISKHRLEELQLELREILDVK*PAITKQLQDAREQGDLSENADYDAAKNEQAALKKRKDEIEEILENYELIEDVLRS
TDEVSIGSTIEIYNYQKDCEEVITLVGSMDSDPFANKISIDTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=143, Percent_Identity=32.1678321678322, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17563; Mature: 17432

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYTISKHRLEELQLELREILDVKPAITKQLQDAREQGDLSENADYDAAKNEQAALKKR
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KDEIEEILENYELIEDVLRSTDEVSIGSTIEIYNYQKDCEEVITLVGSMDSDPFANKISI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEC
DTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID
CCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
AKYTISKHRLEELQLELREILDVKPAITKQLQDAREQGDLSENADYDAAKNEQAALKKR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KDEIEEILENYELIEDVLRSTDEVSIGSTIEIYNYQKDCEEVITLVGSMDSDPFANKISI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEC
DTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID
CCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA