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The map label for this gene is hisS

Identifier: 13357845

GI number: 13357845

Start: 321667

End: 322929

Strand: Direct

Name: hisS

Synonym: UU285

Alternate gene names: 13357845

Gene position: 321667-322929 (Clockwise)

Preceding gene: 13357844

Following gene: 13357846

Centisome position: 42.79

GC content: 25.57

Gene sequence:

>1263_bases
ATGATTAATTACAGTAAACCACGAGGTACTATAGATTTATATAATAAAGAAATGAATGATTTTAAATTACTTGAAAATTT
TTTATTATCAACTACTAAAAGATATGGCTTTCAGCAAATTAAAACACCTGTTTTTGAATTTGCAGAACTCTTTATGAAAT
CAGCTGGTGAAAGTTCAGATTTAGTTTCAAAAGAAATGTATTTATTTAAAGATAAATCAGATCGCTGACTAGCGTTAAGA
CCTGAAGGAACAGCAGGTGTTATTCGTGCTGTTGTTGAGAATAAATTACTATTAAATCATCCACTACCATTAAAATTAAT
GTATTTTGAGCCTTGTTTTCGTTATGAACGCCCTCAAGCTGGACGTCAACGCCAATTTCATCAATTTGGTGTTGAAGTGT
TGGGAACAAAAAATATTTATTATGATTTTGAATTAATTGCTTTAGCTGATACAATTTTAAAAAAATTAATGATATCTAAT
TATATTTTAGAAATTAATTATATCTCATCTCCTCATAATCGTTCATTATGAGTTAAAAGTTTGCAAGAATATTTTCATTT
ACATCGTACTGAATTAACTCCATTAAGTCAAGAACGTATTACAACCAATCCTTTGCGTATTTTAGATGATAAATTAGAAT
CTCAAAAACTAGTTGTAAAACAAGCTCCAAAAATTAGTCATTTTTTATCAAATGAAGAAAAAGAAGAATTTAATCTAATT
AAAAAAATGCTTGATGATTATAACATTAAATATTATGTTAACGAAGGATTAGTAAGAGGTTTGGATTATTATAGTGGATT
AGTATTTGAATTTATTTCAACTTCACCTAAATTATTAGGTCAATCAACAATTATAGGTGGAGGCCGATATGGTGAATTAA
TTAAACAAACAGGTGGCCCTAATTATGAAGGTATTGGTTTTGGTATAGGAATTGAACGTTTATTAATAGCTTTATCTGAA
ACCAATAAAAATATTTTAAACACTGATGATGATAAATATTTAATTGCATTTTTTGATAAAGAATTAGAGAATGAAGCTAT
AAAACTAACTCAAATATTACGTATCAATAATCAATTAAATGTTGACATTATCTTAAGTTCAACTAAAGCTGACAAAATTT
TTAAGTTAGCACAACGTTTAAATGTAAAAAAGCTTATAATATTAGCTAAAAAAGAATGATCAGATAAAAAAATAATTTTA
AAAGATTTACTTAATTTTAAACAAGATTTATTAAGTTTAGATGAAATTAAAGGAATAAGGTAA

Upstream 100 bases:

>100_bases
AACAATTTTTAATCCAGAATATACATCAAAACCTTCAAAGGCAAATCCTTATTTTATTAATTTATTAAAGATGAGTTTAA
AAATAAAAGAAAGTTAGAAT

Downstream 100 bases:

>100_bases
TAAAAATGAAAGTTTATTGTGGTTATATTGGTGAAGAACATTTAGAAAAAAATGTTATTTTAAATGGTTGAGTAAAAAAA
GTTCGTAAAATGGGTAATTT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDRWLALR
PEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISN
YILEINYISSPHNRSLWVKSLQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLI
KKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSE
TNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKEWSDKKIIL
KDLLNFKQDLLSLDEIKGIR

Sequences:

>Translated_420_residues
MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDR*LALR
PEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISN
YILEINYISSPHNRSL*VKSLQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLI
KKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSE
TNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKE*SDKKIIL
KDLLNFKQDLLSLDEIKGIR
>Mature_420_residues
MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDR*LALR
PEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISN
YILEINYISSPHNRSL*VKSLQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLI
KKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSE
TNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKE*SDKKIIL
KDLLNFKQDLLSLDEIKGIR

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=418, Percent_Identity=35.1674641148325, Blast_Score=242, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71993686, Length=446, Percent_Identity=22.6457399103139, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993693, Length=446, Percent_Identity=22.6457399103139, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=396, Percent_Identity=20.959595959596, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48151; Mature: 48151

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSD
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCH
LVSKEMYLFKDKSDRLALRPEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAG
HHHHHHHEEECCCCCEEECCCCHHHHHHHHHHCCCEECCCCCEEEEEECHHHCCCCCCCH
RQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSLVKSLQ
HHHHHHHHCCCEEECCCEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHH
EYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKK
HHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHH
MLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCHHHHHHHHCCCCCC
EGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVD
CCEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEE
IILSSTKADKIFKLAQRLNVKKLIILAKKESDKKIILKDLLNFKQDLLSLDEIKGIR
EEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSD
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCH
LVSKEMYLFKDKSDRLALRPEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAG
HHHHHHHEEECCCCCEEECCCCHHHHHHHHHHCCCEECCCCCEEEEEECHHHCCCCCCCH
RQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSLVKSLQ
HHHHHHHHCCCEEECCCEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHH
EYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKK
HHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHH
MLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCHHHHHHHHCCCCCC
EGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVD
CCEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEE
IILSSTKADKIFKLAQRLNVKKLIILAKKESDKKIILKDLLNFKQDLLSLDEIKGIR
EEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA