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The map label for this gene is pgk

Identifier: 13357839

GI number: 13357839

Start: 314150

End: 315418

Strand: Direct

Name: pgk

Synonym: UU279

Alternate gene names: 13357839

Gene position: 314150-315418 (Clockwise)

Preceding gene: 13357838

Following gene: 13357840

Centisome position: 41.79

GC content: 28.61

Gene sequence:

>1269_bases
ATGAATAGTTTAAATAAAAAAAGTATAAAAGATCTTGATGTAAATGGTAAAACAATAGTTTTACATTTAGATTTAAATGT
TGTTGTTGATTATGAAAATAAACGCATATTAAATGATCGTAAATTACGAGCATCACTACCAACTATTAATTATTTAATTA
ATCATAACGCTAAAATTGTTATTTTAAGTCATTTAGGTCGAATTAAAACCTTAGCAGATAAACAAAGTGGTAAATATAGT
TTAGAAATTATTGTTGATGAACTCCGTAATCGTGTATCCAAGAATGTTAATCGTGTTGTATTTTCACCTTTAAATTATGG
AGAAACGGTCGTACAAATGGTTAATGATTTAGAAGAAAGAGACATTTTAATTTTAGAAAATACGCGTTATTGTGATATTA
GTGATGAAGGTGAATATGTTGGGCTTGAATGAGATGGTTCAGAGATTTTAGGACAGTTTTGGGGAATGCTAGGGGATATT
TTTATTGATGATGCATATGGAGTTGCTCATCGACAATTATCATCAAATTATCAAACAGCTAAATTTGCTAAAAAAAGTGC
ATTAGGTTTTTTAATTGTTAATGAAATTAACCATCTAGATATTGCTTTAGAAGTTCCTAAAAGTCCGTATTTAGCTTTAA
TAGGTGGTAACCGTGTTGCTGATAAAATTGCAGCAATTGAAGTATTATGTGAACGGGCTGATCAAGTTATTATTGGGGGA
GGATTAGTCTATACATTTTTATATGCTCAAGGGTATAACGTTGGTTTAAATTTAGTAGAACGCAATATGATTGATGATTG
CAATAATATATTAGAAAAATACGGTAAAAAAATTTTAATTTGTTTTGATTTTTTATGTAACAATGATTTTAGTGATACAA
GACCGATTTATCGTAAAATAGATGAAGGTCTTGAAGGGTTATATGGTTTAGATATTGGTAAAAGAAGTTTAAAATTTATT
AAACATGAAATTTATCGTTCTAAAACTATTTTACTAAATGGTCCCTTTGGTGTTATTGAAAATATTAAGAATTATGCACA
AGGTACTACTGAAATATGTAAATCAATGGCAAAACAAACAACACGTGGCGCTTATACAATTATATGTGATATCGATACTT
CGAGTCATGCTGAATACCTAGGATTAGATAAATATATTAATTTTATATCTACTGGTGGGGGCGCTAGCTTATCATATATT
GAAGAAGGAGTTCTTGATGGTCTTGAAACCATTGATAATGTTCCTTTAAATGTTTTAAAGAAGGAGTAA

Upstream 100 bases:

>100_bases
TATCAGCACAACGTTATACATTAGTATGATGTGTTAAACAGGTTTTAGCTAATGGTTTGGCAATTATGAAAATAACTCCA
TATGATCAGATGTATTAAAA

Downstream 100 bases:

>100_bases
TTTATGAGTAAATTTAACGAAAAAGAAATTAGAAATAAAGCAATTGAATATATGGAAATATATGGTATGTCACGTCATGA
AGAACGTGTTGCTTCCAAAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYS
LEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLEWDGSEILGQFWGMLGDI
FIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGG
GLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFI
KHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYI
EEGVLDGLETIDNVPLNVLKKE

Sequences:

>Translated_422_residues
MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYS
LEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLE*DGSEILGQFWGMLGDI
FIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGG
GLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFI
KHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYI
EEGVLDGLETIDNVPLNVLKKE
>Mature_422_residues
MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYS
LEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLE*DGSEILGQFWGMLGDI
FIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGG
GLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFI
KHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYI
EEGVLDGLETIDNVPLNVLKKE

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=428, Percent_Identity=32.7102803738318, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI31543397, Length=427, Percent_Identity=31.3817330210773, Blast_Score=196, Evalue=5e-50,
Organism=Escherichia coli, GI1789294, Length=409, Percent_Identity=32.7628361858191, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17508823, Length=425, Percent_Identity=34.8235294117647, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319857, Length=423, Percent_Identity=32.8605200945626, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17136394, Length=426, Percent_Identity=30.2816901408451, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24581203, Length=425, Percent_Identity=30.8235294117647, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 47180; Mature: 47180

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIV
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCEECCCCHHHHCCCEEEEEECCCCEEE
ILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEER
EEECCCHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHC
DILILENTRYCDISDEGEYVGLEDGSEILGQFWGMLGDIFIDDAYGVAHRQLSSNYQTAK
CEEEEECCEEECCCCCCCEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHCCCHHHH
FAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGG
HHHHHCCEEEEEECCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCH
LVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKID
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHCCCCCCCHHHHHHHH
EGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTT
HHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCC
RGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKK
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
E
C
>Mature Secondary Structure
MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIV
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCEECCCCHHHHCCCEEEEEECCCCEEE
ILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEER
EEECCCHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHC
DILILENTRYCDISDEGEYVGLEDGSEILGQFWGMLGDIFIDDAYGVAHRQLSSNYQTAK
CEEEEECCEEECCCCCCCEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHCCCHHHH
FAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGG
HHHHHCCEEEEEECCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCH
LVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKID
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHCCCCCCCHHHHHHHH
EGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTT
HHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCC
RGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKK
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]