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The map label for this gene is celM

Identifier: 13357840

GI number: 13357840

Start: 315422

End: 316513

Strand: Direct

Name: celM

Synonym: UU280

Alternate gene names: 13357840

Gene position: 315422-316513 (Clockwise)

Preceding gene: 13357839

Following gene: 13357841

Centisome position: 41.96

GC content: 31.59

Gene sequence:

>1092_bases
ATGAGTAAATTTAACGAAAAAGAAATTAGAAATAAAGCAATTGAATATATGGAAATATATGGTATGTCACGTCATGAAGA
ACGTGTTGCTTCCAAACTAAAAGCATCATTAAAAGATGCGGGAGTTGATTATGAACGTGATAATTTAGGTTCAATTATTT
TTAAAAAAACAAATTCAAATAAAGGACCTAAAATCTTAATTGCTACACACATGGATGAAGTTGGTTTTGTAGTTCAACAA
ATTCTTGATAATGGTCAATTACTATTATCAATGGTAGGTGGTGTATGGCCTAATATTGCTATTGGATCAGTAGCAAAAGT
GTATGTTGATGAACAACGTCAATATACTGGTGTATTTGGCCATACTTCAATTCATATCTTAGAACCTGAAGCACGCAGTA
AAGCAGTTCCAGTTAAAGAATTATTTGTTGATTGCGGTTTTAATTCAAAACAACAAGCTATTGATTTAGGAATTGAAATA
GGTACGGAAGTTTATATGGAAGGACCAACTTTAAATTTTTATGATGAAAATTATATTGTTGGTAAAGCTGTTGATAATCG
TGTAAGTGTTGCGGTTTTAGATTTATTAATACATAGTTTAAAGGACAAAGTAATTCCCAACCAAACTTATTTTGCTGCAA
CAGTGCAAGAAGAAGTTGGATTACGTGGCGCAAAAACAGTTGTTTCAAAAGTAAAACCTGATATTGGGATTGTTATTGAT
ACAACAACATCACACGATACATACAAGTGTCCAGAGGGTGATACAAAATTAAATGATGGTGTATGTATTAGAATTAAAGA
TGGTGGAACTTTAGTTAATCCTAAATTAGTAAAATATTTTGAAGAATTAGCTATTAAACACAACATTCCTTTATATAAAT
ATGTTGCACGAGGAGGCGGGACTGATGCTGAAGAATTGCAATATGGGCCTAATGGTGGAGTATTAACAATTGGTTTGTCT
ATCCCACAACGTTATTTACATGCTCCAATTGGCGTAGCAACGGTTAAAGATATAAAAGCAGCCTTTGACTTAGTACGTGA
GTTTTTAGAAGTATTTGATGAAAACGAATTTGAAAAAGTAAAATTTAAATAA

Upstream 100 bases:

>100_bases
TGGTGGGGGCGCTAGCTTATCATATATTGAAGAAGGAGTTCTTGATGGTCTTGAAACCATTGATAATGTTCCTTTAAATG
TTTTAAAGAAGGAGTAATTT

Downstream 100 bases:

>100_bases
TTTTTATATCAAATAAAAACCTTTTAATATAAAATTAAATATCGTAATAAATTTATTTTTAGGAGAATAAAATATGACTG
CACATAATCAAGCTAAATTA

Product: endo-1,4-beta-glucanase

Products: D-glucose; GDP

Alternate protein names: TET; TET aminopeptidase; Leucyl aminopeptidase; PhTET2 [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSNKGPKILIATHMDEVGFVVQQ
ILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFGHTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEI
GTEVYMEGPTLNFYDENYIVGKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVID
TTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGGTDAEELQYGPNGGVLTIGLS
IPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKVKFK

Sequences:

>Translated_363_residues
MSKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSNKGPKILIATHMDEVGFVVQQ
ILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFGHTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEI
GTEVYMEGPTLNFYDENYIVGKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVID
TTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGGTDAEELQYGPNGGVLTIGLS
IPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKVKFK
>Mature_362_residues
SKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSNKGPKILIATHMDEVGFVVQQI
LDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFGHTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEIG
TEVYMEGPTLNFYDENYIVGKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVIDT
TTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGGTDAEELQYGPNGGVLTIGLSI
PQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKVKFK

Specific function: Functions as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. However, can also cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. Has neither carboxypeptidase nor endop

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=342, Percent_Identity=32.1637426900585, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082417, Length=352, Percent_Identity=30.1136363636364, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788727, Length=305, Percent_Identity=26.8852459016393, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 40305; Mature: 40174

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
KGPKILIATHMDEVGFVVQQILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFG
CCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHCCCCEEEEECCCCEEEEEC
HTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEIGTEVYMEGPTLNFYDENYIV
CCEEEEECCCCCCCCCCHHHHHEECCCCCCHHHHEECEEECCEEEEECCEEEEECCCEEE
GKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVID
EECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHCCCCCEEEEE
TTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGG
CCCCCCCEECCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHCCCCHHHHHHCCCC
TDAEELQYGPNGGVLTIGLSIPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKV
CCHHHCEECCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
KFK
CCC
>Mature Secondary Structure 
SKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSN
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
KGPKILIATHMDEVGFVVQQILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFG
CCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHCCCCEEEEECCCCEEEEEC
HTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEIGTEVYMEGPTLNFYDENYIV
CCEEEEECCCCCCCCCCHHHHHEECCCCCCHHHHEECEEECCEEEEECCEEEEECCCEEE
GKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVID
EECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHCCCCCEEEEE
TTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGG
CCCCCCCEECCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHCCCCHHHHHHCCCC
TDAEELQYGPNGGVLTIGLSIPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKV
CCHHHCEECCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
KFK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]