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The map label for this gene is spoU-2

Identifier: 13357828

GI number: 13357828

Start: 304282

End: 304800

Strand: Direct

Name: spoU-2

Synonym: UU268

Alternate gene names: 13357828

Gene position: 304282-304800 (Clockwise)

Preceding gene: 13357827

Following gene: 13357829

Centisome position: 40.48

GC content: 25.24

Gene sequence:

>519_bases
ATGTTAAATATTGTTTTATTTGAACCAGAAATTATTTTAAACACTGGTAATATCGCTCGTATGTGTGTAGGTTTTGATGC
AAATTTACATCTTGTTAGACCATATGGATTTATTTTAGATAAAAAACGTTTTCACAAAGATTTTATACGTGCCTCAACCA
ATCATTTAAATGCCTTAAAATTATATGAATATGATGATTTTAATGAATTTATTAATAAAAACAACATTAGTGGTGATGAA
ATATATTTTTTTACTAGATATGGTACTAAAACTCCGTGCGATTATCAATATCAAAATATAATTCAACATAATGTATATTT
AGTTTTTGGTAAAGAATCAACAGGGATAGATCATAATATTTTAAAAAAATATCCTCATAATTGGATCCGTATTCCTACAA
GCATTAATTTGAGAAGTTTAAATATTGCTAATAGTGTTGCTATGGGAATCTATGAAGTAGTGCGGCAAAATAATTTTAAA
GATTTATTCAAATATGAACCTCATAAGAAATTTGAGTAA

Upstream 100 bases:

>100_bases
TTAATAATTAAAGAAAAAAATAAATTAATTAATTTAAAAAAAGAACACGCAAATTTATTAAAAACTCTTACTGACTTAAA
ACAAAAGGTGAAATAATGTT

Downstream 100 bases:

>100_bases
TTTTTATGCGTTTACGAAATAATGCCAATGCTCCACTTTATTTAAAATCACAACACGAATATATAATTAATGATCCACAC
CTTTTAAAAGATAATTTAGG

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALKLYEYDDFNEFINKNNISGDE
IYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNILKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFK
DLFKYEPHKKFE

Sequences:

>Translated_172_residues
MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALKLYEYDDFNEFINKNNISGDE
IYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNILKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFK
DLFKYEPHKKFE
>Mature_172_residues
MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALKLYEYDDFNEFINKNNISGDE
IYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNILKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFK
DLFKYEPHKKFE

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=36.3057324840764, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20377; Mature: 20377

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALK
CEEEEEECCEEEEECCCEEEEEEECCCCEEEECCCCEEECHHHHHHHHHHHCCCCCCEEE
LYEYDDFNEFINKNNISGDEIYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNI
EEEECCHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
LKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFKDLFKYEPHKKFE
HHHCCCCEEECCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALK
CEEEEEECCEEEEECCCEEEEEEECCCCEEEECCCCEEECHHHHHHHHHHHCCCCCCEEE
LYEYDDFNEFINKNNISGDEIYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNI
EEEECCHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
LKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFKDLFKYEPHKKFE
HHHCCCCEEECCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]