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Identifier: 13357829

GI number: 13357829

Start: 304806

End: 305480

Strand: Direct

Name: Not Available

Synonym: UU269

Alternate gene names: 13357829

Gene position: 304806-305480 (Clockwise)

Preceding gene: 13357828

Following gene: 13357830

Centisome position: 40.55

GC content: 24.3

Gene sequence:

>675_bases
ATGCGTTTACGAAATAATGCCAATGCTCCACTTTATTTAAAATCACAACACGAATATATAATTAATGATCCACACCTTTT
AAAAGATAATTTAGGAAAAATTTTTAAAAATCCTGAATTGCCTCTTTATATTGAAATTGGAATGGGTAAGGGTGATTTTA
TTATTGAAAATGCCTTACGAAACCAACAAATTAATTATTTAGGTATTGAAAAATTTCCAACAGTAATTGTTAAAGCTCAC
AAAAAAGCTTTAAAACATAAATTAGATAATTTAGCAATGATTTGTTTTGACGCAAATAAAATTTTAGAATTATTAAATTC
TGAAAGTGTAGATAAAATTTATTTAAATTTTTCTGACCCCTGACCTAAAAAACGTCATGCAAAAAAACGACTGACACATC
CGTGTTTTTTAGAGAAGTTTGCAGTTATTTTAAAACAAAATGCTCTTGTTGAATTTAAAACAGATAATGAAAATTTATTT
ATGTATACTATTTATGATGTATTGCTAAAGGATTTAACAAAGTATGAAATTTTATTTTTAACTTATAATTTATACACACT
TGTTAACAATGTTGAATTATTAAAAAATATTCCTACCGAATATGAAAAAAAATTTGTTATGCAGGGCGAGCGTATTAAAA
AAGTCAATTTTCGTTTTCTTAAAAATAATCAATAA

Upstream 100 bases:

>100_bases
TTGCTAATAGTGTTGCTATGGGAATCTATGAAGTAGTGCGGCAAAATAATTTTAAAGATTTATTCAAATATGAACCTCAT
AAGAAATTTGAGTAATTTTT

Downstream 100 bases:

>100_bases
AAAGATAACTATAATGTTATAATTATATGGTTATATGGCGGTTATGGTGAAGTTGGTTAACACGCCTGATTGTGGCTCAG
GTATTTTCGCGGGTTCGAGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAH
KKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDPWPKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLF
MYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ

Sequences:

>Translated_224_residues
MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAH
KKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP*PKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLF
MYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ
>Mature_224_residues
MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAH
KKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP*PKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLF
MYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26290; Mature: 26290

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALR
CCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
NQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCCEEEEEECCCC
PKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLFMYTIYDVLLKDLTKYEILFLT
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEEE
YNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ
EHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALR
CCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
NQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCCEEEEEECCCC
PKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLFMYTIYDVLLKDLTKYEILFLT
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEEE
YNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ
EHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA