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The map label for this gene is amt-1

Identifier: 13357777

GI number: 13357777

Start: 260648

End: 262180

Strand: Direct

Name: amt-1

Synonym: UU218

Alternate gene names: 13357777

Gene position: 260648-262180 (Clockwise)

Preceding gene: 161760542

Following gene: 13357778

Centisome position: 34.67

GC content: 34.12

Gene sequence:

>1533_bases
GTGAGAATTGTGTCAACAATTATAACTACATCTTGAGGAGTAGATCCAAATATTTACTTTTCAAAGCATGAGTTAACATG
AGTGCCAGCAACAGCTGATGCTAATACATTAGCATTAACTAATTTTAATCCGACTAGCACTTTATTAGTTGCATTAGCAA
TTGCCTTTGTCTTATTGATGACTCCAGGTTTAGCATTATTTTATGGTGGGCTAACACGTCGAAAATCTACATTAACGATT
ATTAATCAATGTGTAGCTTCACTTGGAATCACAACACTGATTTGAATTTTTGGAGGGTTCTCACTTGCTTTTGGACCTTC
TGTTGGTAAAGGAATAATTGGTGATATTTCAACTTATTTTGCATTTAGAAATCTATTATTTGCTGATGGTTGAAGTGGAG
ATGTAGGAATTGTTTTTGCAAACTTTACTAATGGTGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATT
ATTACACCACCATTAATGGTTGGTGCATTTGCAGATCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACA
ATATTTAATTTACATTCCTTTTGCACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTG
GTGGTATTGTAATTCATACAACTGCTGGTTTTGGAGCATTAGCTGCTTCATTAGTATTAGGTAAACGTGTTTTACTTAAG
AATGATAAAAGTCGTCCACACAATATTCCAATGACTATTATTGGAGCAACATTGTTATTCTTTGGTTGATTTGGATTTAA
TGTTGGTGGATCAGGATTTGTAGCTGGTGGGAATGCTGCTACTTGATCAGCTGCTACAAGTGCTTGGATTAATACAATTA
TTGCTTTAGCTATTGGAATGGTTGGTTGAGCAGTTCTTGAAACAATCTTTAATAAAAATCATAAAGCCACAGGTGTTGGT
TTAGTAACAGGTGCAATTGCTGGATTAGCAACAATTACTCCTGCAGCAGGTTATGTTCCAATTTGAGCATCTGTTCCTAT
TGGTGTAGCTGCTGTGCTAGTTTGTTATACTAGTGCTAAAACTTTACATCATTTCCACAAAATTGATGATACTCTAGAAG
TATGAGGCGTACATGGAATGGGTGGTGTTACTGGTTCATTATTAATTGGTGCGTTTGCATCTAAATCTGTAAACCCATCA
ATTATGTATGAAGCAATTGGTCCAACAACAACTGGTGTTTTATTTGGTGTCCAATTAGGAGCGGTTTTATTAGCTGCGGT
TTATGGATTTGTATTTACTATTTTATTAGTATATATTACTCGTCCAAGATTATCAGCACGCCAACAATTAGGTCATATAG
ACTATATTAATCATGGCGAAGATGCATATTCATTCGATATTGAAATTCCATCTGAAAAAGAAATGGAGCAATATGAATTA
GCAGATAGTACATGACACTCAAGCACAGGTGTTAAGTATTCATTGCTTAATAATAAAAACCAGCAAAATCATAAAGTAGC
AAACGACCATTAA

Upstream 100 bases:

>100_bases
TAATTCGCTTGTGCTTGCTTTTTATTTTTTTAGTACTATAATAGTAATATGTACTTAAAAATTATATTTTGTTTTAAAGT
ATAAAAAAAATAAATAAGAG

Downstream 100 bases:

>100_bases
TTTTTAAAAAAAGACTAAAGCAAAATACTTTAGTTTTTTTAATTAATAAATAATAAAAAAATAAAGAATTTATTAATTAA
CGTAATATAATACTATAGAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRIVSTIITTSWGVDPNIYFSKHELTWVPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTI
INQCVASLGITTLIWIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGWSGDVGIVFANFTNGVPLILFFAYQLAFAI
ITPPLMVGAFADRMKFKNYLVFLVLWQYLIYIPFAHWIWGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLK
NDKSRPHNIPMTIIGATLLFFGWFGFNVGGSGFVAGGNAATWSAATSAWINTIIALAIGMVGWAVLETIFNKNHKATGVG
LVTGAIAGLATITPAAGYVPIWASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEVWGVHGMGGVTGSLLIGAFASKSVNPS
IMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYEL
ADSTWHSSTGVKYSLLNNKNQQNHKVANDH

Sequences:

>Translated_510_residues
MRIVSTIITTS*GVDPNIYFSKHELT*VPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTI
INQCVASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGDVGIVFANFTNGVPLILFFAYQLAFAI
ITPPLMVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLK
NDKSRPHNIPMTIIGATLLFFG*FGFNVGGSGFVAGGNAAT*SAATSAWINTIIALAIGMVG*AVLETIFNKNHKATGVG
LVTGAIAGLATITPAAGYVPI*ASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV*GVHGMGGVTGSLLIGAFASKSVNPS
IMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYEL
ADST*HSSTGVKYSLLNNKNQQNHKVANDH
>Mature_510_residues
MRIVSTIITTS*GVDPNIYFSKHELT*VPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTI
INQCVASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGDVGIVFANFTNGVPLILFFAYQLAFAI
ITPPLMVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLK
NDKSRPHNIPMTIIGATLLFFG*FGFNVGGSGFVAGGNAAT*SAATSAWINTIIALAIGMVG*AVLETIFNKNHKATGVG
LVTGAIAGLATITPAAGYVPI*ASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV*GVHGMGGVTGSLLIGAFASKSVNPS
IMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYEL
ADST*HSSTGVKYSLLNNKNQQNHKVANDH

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=447, Percent_Identity=33.5570469798658, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=27.6548672566372, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17550180, Length=450, Percent_Identity=24.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324187, Length=497, Percent_Identity=32.7967806841046, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321559, Length=488, Percent_Identity=27.2540983606557, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=464, Percent_Identity=26.9396551724138, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=24.6973365617433, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52075; Mature: 52075

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVSTIITTSGVDPNIYFSKHELTVPATADANTLALTNFNPTSTLLVALAIAFVLLMTP
CCHHHHHHHCCCCCCCEEEECCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
GLALFYGGLTRRKSTLTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFR
CHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHH
NLLFADGSGDVGIVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLV
HHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQYLIYIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDKSRPH
HHHHHHCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCC
NIPMTIIGATLLFFGFGFNVGGSGFVAGGNAATSAATSAWINTIIALAIGMVGAVLETIF
CCCHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKNHKATGVGLVTGAIAGLATITPAAGYVPIASVPIGVAAVLVCYTSAKTLHHFHKIDDT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCH
LEVGVHGMGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVF
HHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
TILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADSTHSSTGVKY
HHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEE
SLLNNKNQQNHKVANDH
EEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRIVSTIITTSGVDPNIYFSKHELTVPATADANTLALTNFNPTSTLLVALAIAFVLLMTP
CCHHHHHHHCCCCCCCEEEECCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
GLALFYGGLTRRKSTLTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFR
CHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHH
NLLFADGSGDVGIVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLV
HHEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQYLIYIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDKSRPH
HHHHHHCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCC
NIPMTIIGATLLFFGFGFNVGGSGFVAGGNAATSAATSAWINTIIALAIGMVGAVLETIF
CCCHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKNHKATGVGLVTGAIAGLATITPAAGYVPIASVPIGVAAVLVCYTSAKTLHHFHKIDDT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCH
LEVGVHGMGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVF
HHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
TILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADSTHSSTGVKY
HHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEE
SLLNNKNQQNHKVANDH
EEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]