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The map label for this gene is amt-2

Identifier: 13357778

GI number: 13357778

Start: 262345

End: 263910

Strand: Direct

Name: amt-2

Synonym: UU219

Alternate gene names: 13357778

Gene position: 262345-263910 (Clockwise)

Preceding gene: 13357777

Following gene: 13357779

Centisome position: 34.9

GC content: 33.14

Gene sequence:

>1566_bases
GTGAAAATTGTGTCAACTTGAGGCCTAAATAAAGATATTTATTTCTCAACTCATGAATTAACATGAGTGCCCGCAGATGC
AAGTCAATCAACACTTAATGCAATAGGTTTTAATCCTACTAATACACTACTAGTAGCTTTAGCAATTGCTTTTGTTTTAC
TAATGACACCAGGGTTAGCATTATTTTATGGTGGTTTAGTAAGACGTAAATCAGCTTTAACAATTATTAATCAATGTGTA
GCTTCACTTGGAATTACAACATTGATTTGAATTTTTGGAGGTTTCTCACTTGCTTTTGGACCTTCTGTTGGTAAAGGAAT
AATTGGTGATATTTCAACTTATTTTGCATTTAGAAATCTACTATTTGCTGATGGTTGAAGTGGAGGAGCAGTTTTAGTTT
TTGCTAATTTTACTAACGGCGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATTATTACACCACCATTA
ATGGTTGGTGCATTTGCAGACCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACAATATTTAATTTATAT
TCCTTTTGCACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTGGTGGTATTGTAATTC
ATACAACTGCTGGTTTTGGAGCTTTGGCTGCTTCATTTATGTTAGGAAAACGTACAACATTAATTACAGATAAAAAGCGA
GCTAATAATTTACCAATGGTTGTATTAGGTGCAGCATTACTATTTTTTGGTTGATTTGGATTTAATGTTGGTGGTGCAGG
TTTTGTTAAAGAAAATACAAATCACATTTCACAAGCTATATCATCATGATTAAATACAATTATTGCCTTAGCAATAGGTA
TGATAGGTTGAGTTACACTTGAAACTATTGTTAACAAAAACCACAAACCAACAACAGTAGGTTTAGTGACTGGTGCAGTC
GCTGGATTAGCAACAATTACTCCCGCAGCAGGTTTTGTTCCAGTTTGAGCCTCTGTTCCTATTGCCATTATGGGTGTTTT
AGTATGTTATTCAGTTTCTAGATTATTAGATTACTTCCGTTTTGATGATTCTTTAGAAGTTCTACCAGTTCATGGAATGG
GTGGTGTTATAGGTTCTTTATTAATTGGAGCATTTGCTACTAACACGGTTGCACATGGAATTAAATATGACGCCGTCGGA
TTAGATAATAATGGAAATCCAATTTATGGTATTTTATTTGGACTACAATTAGGTGCTGTATTATTAGCAGCAACATGGGC
ATTTGCCTTTACATGTTTAATAGTTTATATTACTAAACCTCGTTTATCAGTGCGTCAACAATTAGGCCATATAGACTACA
TCAATCATGGTGAAGATGCATACAAGTTTGATTTTATTATTCCTTCAGAAAGTGAAATGTTTGAATATGAAAGTTTTGAT
CAAACTTGAGAATGTAAAGCTAGTGGTTGTAAAAAAAGCAACTTAGCTGCCAAGAAAAACCCTGTTTTTATAGAGAATGA
GAATGATTGTACATGACAATCTAAATCTCGTGAGTGTACAAAATAG

Upstream 100 bases:

>100_bases
AGAATTTATTAATTAACGTAATATAATACTATAGATATATTTTGTATTTATAAATTTATGTACAAAATTTATTTATCGAA
AACGAAAAAGAAAACACGAG

Downstream 100 bases:

>100_bases
AAAATATCAGTATTTCATAATTAAGAAGTTAATTAAAACTAACTTCTTTTTTATTTATAATTTATAATTGATGAAATAGT
AATATTTATCTTAAATGGGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MKIVSTWGLNKDIYFSTHELTWVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCV
ASLGITTLIWIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGWSGGAVLVFANFTNGVPLILFFAYQLAFAIITPPL
MVGAFADRMKFKNYLVFLVLWQYLIYIPFAHWIWGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKR
ANNLPMVVLGAALLFFGWFGFNVGGAGFVKENTNHISQAISSWLNTIIALAIGMIGWVTLETIVNKNHKPTTVGLVTGAV
AGLATITPAAGFVPVWASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVG
LDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFD
QTWECKASGCKKSNLAAKKNPVFIENENDCTWQSKSRECTK

Sequences:

>Translated_521_residues
MKIVST*GLNKDIYFSTHELT*VPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCV
ASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGGAVLVFANFTNGVPLILFFAYQLAFAIITPPL
MVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKR
ANNLPMVVLGAALLFFG*FGFNVGGAGFVKENTNHISQAISS*LNTIIALAIGMIG*VTLETIVNKNHKPTTVGLVTGAV
AGLATITPAAGFVPV*ASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVG
LDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFD
QT*ECKASGCKKSNLAAKKNPVFIENENDCT*QSKSRECTK
>Mature_521_residues
MKIVST*GLNKDIYFSTHELT*VPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCV
ASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGGAVLVFANFTNGVPLILFFAYQLAFAIITPPL
MVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKR
ANNLPMVVLGAALLFFG*FGFNVGGAGFVKENTNHISQAISS*LNTIIALAIGMIG*VTLETIVNKNHKPTTVGLVTGAV
AGLATITPAAGFVPV*ASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVG
LDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFD
QT*ECKASGCKKSNLAAKKNPVFIENENDCT*QSKSRECTK

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=33.4128878281623, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=27.0642201834862, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=22.9910714285714, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564042, Length=360, Percent_Identity=24.7222222222222, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324187, Length=436, Percent_Identity=32.5688073394495, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325396, Length=490, Percent_Identity=27.7551020408163, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321559, Length=352, Percent_Identity=29.5454545454545, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078314, Length=429, Percent_Identity=25.8741258741259, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 53819; Mature: 53819

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSTGLNKDIYFSTHELTVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALF
CCEECCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHH
YGGLVRRKSALTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHEEE
DGSGGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQYLI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANNLPMV
HCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEECHHHCCCCCHH
VLGAALLFFGFGFNVGGAGFVKENTNHISQAISSLNTIIALAIGMIGVTLETIVNKNHKP
HHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTVGLVTGAVAGLATITPAAGFVPVASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVH
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GMGGVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFT
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQTECKASGCKK
HHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCCC
SNLAAKKNPVFIENENDCTQSKSRECTK
CCCCCCCCCEEEECCCCHHCCCCCCCCC
>Mature Secondary Structure
MKIVSTGLNKDIYFSTHELTVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALF
CCEECCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHH
YGGLVRRKSALTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHEEE
DGSGGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQYLI
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANNLPMV
HCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEECHHHCCCCCHH
VLGAALLFFGFGFNVGGAGFVKENTNHISQAISSLNTIIALAIGMIGVTLETIVNKNHKP
HHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTVGLVTGAVAGLATITPAAGFVPVASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVH
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GMGGVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFT
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQTECKASGCKK
HHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCCC
SNLAAKKNPVFIENENDCTQSKSRECTK
CCCCCCCCCEEEECCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]