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The map label for this gene is kinE [H]

Identifier: 15595109

GI number: 15595109

Start: 805775

End: 806923

Strand: Reverse

Name: kinE [H]

Synonym: BB0764

Alternate gene names: 15595109

Gene position: 806923-805775 (Counterclockwise)

Preceding gene: 15595110

Following gene: 15595108

Centisome position: 88.6

GC content: 25.5

Gene sequence:

>1149_bases
ATGAATAATTTTTTCAAAAAAGCTTTAACAAAGCTAAACAAATTATCTAACGAACAAAAAACTAAATTTATTGAACAAAT
TTACAAAAAAATAGAAATATATGACGGAATATTTGCATCAATTAATGAAGGAATCATTGTACTTGACAAACAAAACAATA
TAATCTATGCAAACAAGATTTTATACCAAATTTTAGCTTTAACATCTAAATCAAAAATAGAAATTCTTGATGACATTCAA
ATTCCAAACTTAATAAATTTAATAAAAGAACTAGTTAGAACAGAAGATAAAATAATAGGATTAGAAGTTCCAATCTCAAA
CGGCATATATATTAAAATCTCATTTATGCCTTATGTAAAAGAAAAAAAACTTGAAGGCAACATTATTTTAATCGAAGACA
TTAAAGAGAAAAAAAAGAAAGAGGAACTATTTAGAAGAGTTGAGGCTTTGGCCTCTTTTACAAGGCATGCAAGAAATATT
GCCCATGAAATCAAAAACCCACTTGGAGCAATCGATATAAATTTACAACTGCTAAAAAAGGAAATTGAAAAACAAAAAAT
GAAAAATGGTAAAGCTGAAAATTATTTTAAAGTAATAAAAGAAGAAATAAACAGAGTAGATAAAATAGTAACAGAATTTT
TACTAACTGTCAGACCAATAAAAATTAACTTACAAGAAAAAGATATTAAACAAGTAATAGGCAGCGTATGTGAATTGTTA
AATCCTGGATTAGAAAATAAACACATAAAACTATTGCTTAATTTAAACAAAATAAGCAATATTCTCATTGATGAAAAACT
ATTAAAACAAGTTATTATAAACATCGTTAAAAACGCAGAAGAAGCACTGCTTGAAACAAAAAAAGAAATAAAAAAAATAG
AAATTTTTCTCTTCGAAAAAGACAATAAAATACATATCAACATAAAAGATAACGGAAACGGAATAAAAGATGGGGTAAAA
GAGGAAATATTTAAGCCTCAATTTAGCACAAAAGAAAAAGGAAGTGGAATAGGACTTACTATTTCTTATAAAATAATAAA
AGAGCTTGGAGGTGAAATTTTTGTGGAAAGCAAAGAGGGCAAAGGCACTATTTTTACAATTACGCTGCCTAAACTAAATA
AAAAAAATATTTTAATTGAAGGGTATTGA

Upstream 100 bases:

>100_bases
TAAAGTACACAGAGCATTTGAAAAATATTTACAAATATCGCCCCTATAATCAAAATAAAAAATTAATAATAGAAAACTTA
ATGCTAAATTATGAGGAGAT

Downstream 100 bases:

>100_bases
AAATGAGCAAAATACTTGTAGCTGATGATGAAAAGAATATTAGAGAAGGAATTGCTACTTATCTTGAGGATGAAGGATAT
TTTGTTTTCACTGCTAGTGA

Product: sensory transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNNFFKKALTKLNKLSNEQKTKFIEQIYKKIEIYDGIFASINEGIIVLDKQNNIIYANKILYQILALTSKSKIEILDDIQ
IPNLINLIKELVRTEDKIIGLEVPISNGIYIKISFMPYVKEKKLEGNIILIEDIKEKKKKEELFRRVEALASFTRHARNI
AHEIKNPLGAIDINLQLLKKEIEKQKMKNGKAENYFKVIKEEINRVDKIVTEFLLTVRPIKINLQEKDIKQVIGSVCELL
NPGLENKHIKLLLNLNKISNILIDEKLLKQVIINIVKNAEEALLETKKEIKKIEIFLFEKDNKIHINIKDNGNGIKDGVK
EEIFKPQFSTKEKGSGIGLTISYKIIKELGGEIFVESKEGKGTIFTITLPKLNKKNILIEGY

Sequences:

>Translated_382_residues
MNNFFKKALTKLNKLSNEQKTKFIEQIYKKIEIYDGIFASINEGIIVLDKQNNIIYANKILYQILALTSKSKIEILDDIQ
IPNLINLIKELVRTEDKIIGLEVPISNGIYIKISFMPYVKEKKLEGNIILIEDIKEKKKKEELFRRVEALASFTRHARNI
AHEIKNPLGAIDINLQLLKKEIEKQKMKNGKAENYFKVIKEEINRVDKIVTEFLLTVRPIKINLQEKDIKQVIGSVCELL
NPGLENKHIKLLLNLNKISNILIDEKLLKQVIINIVKNAEEALLETKKEIKKIEIFLFEKDNKIHINIKDNGNGIKDGVK
EEIFKPQFSTKEKGSGIGLTISYKIIKELGGEIFVESKEGKGTIFTITLPKLNKKNILIEGY
>Mature_382_residues
MNNFFKKALTKLNKLSNEQKTKFIEQIYKKIEIYDGIFASINEGIIVLDKQNNIIYANKILYQILALTSKSKIEILDDIQ
IPNLINLIKELVRTEDKIIGLEVPISNGIYIKISFMPYVKEKKLEGNIILIEDIKEKKKKEELFRRVEALASFTRHARNI
AHEIKNPLGAIDINLQLLKKEIEKQKMKNGKAENYFKVIKEEINRVDKIVTEFLLTVRPIKINLQEKDIKQVIGSVCELL
NPGLENKHIKLLLNLNKISNILIDEKLLKQVIINIVKNAEEALLETKKEIKKIEIFLFEKDNKIHINIKDNGNGIKDGVK
EEIFKPQFSTKEKGSGIGLTISYKIIKELGGEIFVESKEGKGTIFTITLPKLNKKNILIEGY

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=30.2521008403361, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=30.4878048780488, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790300, Length=353, Percent_Identity=26.3456090651558, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994928, Length=286, Percent_Identity=23.7762237762238, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=25.5230125523013, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786600, Length=347, Percent_Identity=21.0374639769452, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786837, Length=383, Percent_Identity=22.7154046997389, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=354, Percent_Identity=23.4463276836158, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789149, Length=274, Percent_Identity=24.8175182481752, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790567, Length=345, Percent_Identity=24.0579710144928, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43960; Mature: 43960

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFFKKALTKLNKLSNEQKTKFIEQIYKKIEIYDGIFASINEGIIVLDKQNNIIYANKI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCEEEHHHH
LYQILALTSKSKIEILDDIQIPNLINLIKELVRTEDKIIGLEVPISNGIYIKISFMPYVK
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCHH
EKKLEGNIILIEDIKEKKKKEELFRRVEALASFTRHARNIAHEIKNPLGAIDINLQLLKK
HCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
EIEKQKMKNGKAENYFKVIKEEINRVDKIVTEFLLTVRPIKINLQEKDIKQVIGSVCELL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHH
NPGLENKHIKLLLNLNKISNILIDEKLLKQVIINIVKNAEEALLETKKEIKKIEIFLFEK
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEEEEC
DNKIHINIKDNGNGIKDGVKEEIFKPQFSTKEKGSGIGLTISYKIIKELGGEIFVESKEG
CCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEEECCCC
KGTIFTITLPKLNKKNILIEGY
CEEEEEEECCCCCCCCEEEECC
>Mature Secondary Structure
MNNFFKKALTKLNKLSNEQKTKFIEQIYKKIEIYDGIFASINEGIIVLDKQNNIIYANKI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCEEEHHHH
LYQILALTSKSKIEILDDIQIPNLINLIKELVRTEDKIIGLEVPISNGIYIKISFMPYVK
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCHH
EKKLEGNIILIEDIKEKKKKEELFRRVEALASFTRHARNIAHEIKNPLGAIDINLQLLKK
HCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
EIEKQKMKNGKAENYFKVIKEEINRVDKIVTEFLLTVRPIKINLQEKDIKQVIGSVCELL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHH
NPGLENKHIKLLLNLNKISNILIDEKLLKQVIINIVKNAEEALLETKKEIKKIEIFLFEK
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEEEEC
DNKIHINIKDNGNGIKDGVKEEIFKPQFSTKEKGSGIGLTISYKIIKELGGEIFVESKEG
CCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEEECCCC
KGTIFTITLPKLNKKNILIEGY
CEEEEEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]