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The map label for this gene is dnaX [C]

Identifier: 15595110

GI number: 15595110

Start: 806920

End: 807966

Strand: Reverse

Name: dnaX [C]

Synonym: BB0765

Alternate gene names: 15595110

Gene position: 807966-806920 (Counterclockwise)

Preceding gene: 15595111

Following gene: 15595109

Centisome position: 88.72

GC content: 23.69

Gene sequence:

>1047_bases
ATGACAATGCTTCCAAAAATTGCAAAAGAGATAATAAACGAATATGATCAAAAAATACTGCCAAATGCAATTCTTTTACT
AGGAGAAAAATTTTCTTCAAAAAAGATTAGCGCAATTGAGCTTGCAAAAAAAATATTAAACGGAAAAAACTTAACAAACC
CTAATTTGCTCATTTTCTCAAATCTTGACACAGTAGAAGCAAAAGCACATCTTTCTACAAATTCGCAAAAGATAGCAAAT
AAATACCTAGAATATATTAAAACTGTAATTTTTACCAAATGTTATTTCAGCAATGAAAAAAATTTAAAAAAAATAGAAAA
AAATATCAACTACATTAATTCTGTTTATTATGAAAAAGAATACAATGAAAACATAAAAAATGAGCTTATAAAAAATATAG
AAAATATAAACAAAGAATTAAATCATAGCATTACTGTTTATGATGTAAAAAAAATTCAAACTTGGATTTTTTCTGAAAAA
GAAAAACCAAAGGTAATCTACATAAACGAAATCGAAAATTTATCATTTAATGTCCATAACTCACTTTTAAAAATATTGGA
AGAGCCTCCCTCAAATATTTACTTTATCTTGGCAGCAAGAAATAAAAACAAAATACCAAAAACAATACTTTCAAGACTTA
GAGTCTACAATTTCGCAAAACTAGACAGAAGCTTAGAAATTCAAAGATTTAAAGAAAGCTTTCTAATAAATAAAGATATA
ACAATTGAAGAGTATTTCGCCTCATTTTACAAAGAAGAAAGCAAAAAAATAAAAAAAGAATTGGCAAAAATTCTAAATAT
AATAAAAGAAAAAAAATCCATATTTAATCTTGAAGAAGTCGACTTTATAAAAGATGAGCAAAGCTTTAAAATATTTTTAA
ACGAACTTACAATTAACATTAGAAAAGATTTTTTAGAAAACAAAATAGATATTAATCAATATCTAAAGTACACAGAGCAT
TTGAAAAATATTTACAAATATCGCCCCTATAATCAAAATAAAAAATTAATAATAGAAAACTTAATGCTAAATTATGAGGA
GATATGA

Upstream 100 bases:

>100_bases
TAAACCTGAATATTTCCTAAAAGAAAGCAAACTACTTGATTATTTAAATCCTGGAATAAACTATCTCTTTAAAATTTCAA
AAACAAAATAAGGGCCAGCA

Downstream 100 bases:

>100_bases
ATAATTTTTTCAAAAAAGCTTTAACAAAGCTAAACAAATTATCTAACGAACAAAAAACTAAATTTATTGAACAAATTTAC
AAAAAAATAGAAATATATGA

Product: hypothetical protein

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Gamma And Tau Subunit; DNA Polymerase III Gamma/Tau Subunits

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTMLPKIAKEIINEYDQKILPNAILLLGEKFSSKKISAIELAKKILNGKNLTNPNLLIFSNLDTVEAKAHLSTNSQKIAN
KYLEYIKTVIFTKCYFSNEKNLKKIEKNINYINSVYYEKEYNENIKNELIKNIENINKELNHSITVYDVKKIQTWIFSEK
EKPKVIYINEIENLSFNVHNSLLKILEEPPSNIYFILAARNKNKIPKTILSRLRVYNFAKLDRSLEIQRFKESFLINKDI
TIEEYFASFYKEESKKIKKELAKILNIIKEKKSIFNLEEVDFIKDEQSFKIFLNELTINIRKDFLENKIDINQYLKYTEH
LKNIYKYRPYNQNKKLIIENLMLNYEEI

Sequences:

>Translated_348_residues
MTMLPKIAKEIINEYDQKILPNAILLLGEKFSSKKISAIELAKKILNGKNLTNPNLLIFSNLDTVEAKAHLSTNSQKIAN
KYLEYIKTVIFTKCYFSNEKNLKKIEKNINYINSVYYEKEYNENIKNELIKNIENINKELNHSITVYDVKKIQTWIFSEK
EKPKVIYINEIENLSFNVHNSLLKILEEPPSNIYFILAARNKNKIPKTILSRLRVYNFAKLDRSLEIQRFKESFLINKDI
TIEEYFASFYKEESKKIKKELAKILNIIKEKKSIFNLEEVDFIKDEQSFKIFLNELTINIRKDFLENKIDINQYLKYTEH
LKNIYKYRPYNQNKKLIIENLMLNYEEI
>Mature_347_residues
TMLPKIAKEIINEYDQKILPNAILLLGEKFSSKKISAIELAKKILNGKNLTNPNLLIFSNLDTVEAKAHLSTNSQKIANK
YLEYIKTVIFTKCYFSNEKNLKKIEKNINYINSVYYEKEYNENIKNELIKNIENINKELNHSITVYDVKKIQTWIFSEKE
KPKVIYINEIENLSFNVHNSLLKILEEPPSNIYFILAARNKNKIPKTILSRLRVYNFAKLDRSLEIQRFKESFLINKDIT
IEEYFASFYKEESKKIKKELAKILNIIKEKKSIFNLEEVDFIKDEQSFKIFLNELTINIRKDFLENKIDINQYLKYTEHL
KNIYKYRPYNQNKKLIIENLMLNYEEI

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 41485; Mature: 41354

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLPKIAKEIINEYDQKILPNAILLLGEKFSSKKISAIELAKKILNGKNLTNPNLLIFS
CCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
NLDTVEAKAHLSTNSQKIANKYLEYIKTVIFTKCYFSNEKNLKKIEKNINYINSVYYEKE
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YNENIKNELIKNIENINKELNHSITVYDVKKIQTWIFSEKEKPKVIYINEIENLSFNVHN
HCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
SLLKILEEPPSNIYFILAARNKNKIPKTILSRLRVYNFAKLDRSLEIQRFKESFLINKDI
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
TIEEYFASFYKEESKKIKKELAKILNIIKEKKSIFNLEEVDFIKDEQSFKIFLNELTINI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHH
RKDFLENKIDINQYLKYTEHLKNIYKYRPYNQNKKLIIENLMLNYEEI
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMLPKIAKEIINEYDQKILPNAILLLGEKFSSKKISAIELAKKILNGKNLTNPNLLIFS
CCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
NLDTVEAKAHLSTNSQKIANKYLEYIKTVIFTKCYFSNEKNLKKIEKNINYINSVYYEKE
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YNENIKNELIKNIENINKELNHSITVYDVKKIQTWIFSEKEKPKVIYINEIENLSFNVHN
HCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
SLLKILEEPPSNIYFILAARNKNKIPKTILSRLRVYNFAKLDRSLEIQRFKESFLINKDI
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
TIEEYFASFYKEESKKIKKELAKILNIIKEKKSIFNLEEVDFIKDEQSFKIFLNELTINI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHH
RKDFLENKIDINQYLKYTEHLKNIYKYRPYNQNKKLIIENLMLNYEEI
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA