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The map label for this gene is fruA [H]

Identifier: 15594974

GI number: 15594974

Start: 659644

End: 661524

Strand: Reverse

Name: fruA [H]

Synonym: BB0629

Alternate gene names: 15594974

Gene position: 661524-659644 (Counterclockwise)

Preceding gene: 15594976

Following gene: 15594972

Centisome position: 72.64

GC content: 32.96

Gene sequence:

>1881_bases
GTGATTATTATGCAAAATTTATTTTCAAAAAACTTGATTGTTTTAAATTACAATGCAACTAGTAAAGAAGATGTAATTAG
AAAAATGGCTAGCATGTTCAATGAAAATGGATACTTAAATGACATGGAAGCATTTATAAAAGAAATTAAAAAAAGAGAAG
AAACTAACGGAACAGGCATTGAAGAGCATATAGCTATGCCTCATGCAAAAGGCAATTTCATTAAAAAACACGGAATTGCT
ATTTTAAGAGTTGTTGGCAATGGTTTTGACTTCAACTCTTCTGATCAAAAGCTTTCAAAACTGTTTTTCATGATGGCCCT
ACCCGAAGAAACTCCAAGCAATGCACACATAAAAGCTATATCATACCTAAGTAATACTTTTAGCAACAACCTATTAAGAC
ATGAACTTATGAGCACAAATAATGAAGATAGATTTTTAGAAATAATATTGAATAATGACAATATAAATGAATCTAACAAT
TTAAATACAAAAAAAGATTTCATTCTTGCCGTAACAGCATGTCCTGTGGGAATAGCTCACACCTATATGGCAGCAGAAAG
CCTTAAAAAAGCAGCTTTAGAATTAAACATAAATATAAAAGTAGAAACAAATGGATCTAGTGGAACCGAAAATCCAATAA
CAGAAGAAGAAATAAAAAAAGCAAAAGGAGTCATTATTGCATCTGGCAAAACTATCGATAAAGAAAGATTTAGCGGAAAA
CCTTTAATCGAAGTGGGAGTAAAAGACGGCATACACAAAGCAAAAGAGCTTATCCAAACAATTCTTAAAAACGAAGCACC
AATTTACAAAAAAAGCAACACAAACAAAACCACCGAAACCCTCCAAAAACAAAACAAAAAAACGGGGATTTATAAACATC
TAATGAATGGGGTCTCATTTATGCTTCCATTTGTAGTCTCAGGAGGAATAATAATAGCAATATCATTCATGTTTGGAATC
AAAGCATTTGACATAAACGATCCAAGCTACAATAAAATAGCAGATATTCTAATGCAAATCGGCGGTGGAAGCGCATTTGC
TTTAATGATCCCAATACTTGCTGGCTATATTTCATTTAGCATAGCAGAAAGACCAGGACTTGCACCTGGAATGATTACAG
GATTAATGATGAACAATGGAAATGCAGGATTTTTGGGAGGCATCTTAGCAGGATTTATTTCAGGCTACGTTACACTAACT
GTAAAAAAAATATCTGACAAAATAATTCCTAGCAATTTAAGAGGAATAAATCCGGTATTAACTTATCCTTTTTTATCAGT
TATAATTTCAGGAATTTTGATATATGGAATGCTTAGTCCAATATCTGTTATTAATGAATCAATAACAAATATGCTAAATC
AACTTAGCGGTACTAATATGGCAATACTTGGAGCTTTGCTTGGAGGAATGATGGCAATAGATATGGGAGGGCCTGTAAAT
AAAGCTGCATATGCATTTGGAATTGCAATGATAACTGCCAAAAATTATATTCCTCACGCAAGCATAATGGCAGGAGGAAT
GATACCTCCCATAGGAATTGCTCTTGCTACAAGTTTATTTAAAAATAGATTCTCAAAAGAAGAAAGAGAATCTGGAAAAG
TTTGTTATTTTTTGGGAGCATGCTTTATTACAGAAGGAGTAATTCCATTTGCAGCAGCAGATCCTTTAAGGGTAATACCC
GCATGTATACTAGGCTCATCTGTAGGAGGATTTATTTCTGCACTTTTCAAGGTAGAGGTTATAGCACCACACGGCGGAAT
ATTTATTCTACCAATAGTAGTAAACCCATTAATGTGGATAACATCTATCCTGGTAGGATCTATTATAACAGCTGTTTTAA
TAGGAATCCTTAAAAAAGAATACAAGAATATAAACGATTAA

Upstream 100 bases:

>100_bases
GTGTTAGAGTATATATCAAGATAATCTCCTTTGATTTATAAAATCAATTAAATAAATAGTAAAATTTAATAAATGTATAT
ACACTATATAATATATAAAG

Downstream 100 bases:

>100_bases
GTTTATATTCTTGAACCCAGTCAATAAACTAATCTAAGTTTTATTGGTGTTTCAAGACTTAATCCTTTAACCATATCCAT
CTCAAACCGCAGTGTGTTTG

Product: PTS system, fructose-specific IIABC component (fruA-2)

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MIIMQNLFSKNLIVLNYNATSKEDVIRKMASMFNENGYLNDMEAFIKEIKKREETNGTGIEEHIAMPHAKGNFIKKHGIA
ILRVVGNGFDFNSSDQKLSKLFFMMALPEETPSNAHIKAISYLSNTFSNNLLRHELMSTNNEDRFLEIILNNDNINESNN
LNTKKDFILAVTACPVGIAHTYMAAESLKKAALELNINIKVETNGSSGTENPITEEEIKKAKGVIIASGKTIDKERFSGK
PLIEVGVKDGIHKAKELIQTILKNEAPIYKKSNTNKTTETLQKQNKKTGIYKHLMNGVSFMLPFVVSGGIIIAISFMFGI
KAFDINDPSYNKIADILMQIGGGSAFALMIPILAGYISFSIAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLT
VKKISDKIIPSNLRGINPVLTYPFLSVIISGILIYGMLSPISVINESITNMLNQLSGTNMAILGALLGGMMAIDMGGPVN
KAAYAFGIAMITAKNYIPHASIMAGGMIPPIGIALATSLFKNRFSKEERESGKVCYFLGACFITEGVIPFAAADPLRVIP
ACILGSSVGGFISALFKVEVIAPHGGIFILPIVVNPLMWITSILVGSIITAVLIGILKKEYKNIND

Sequences:

>Translated_626_residues
MIIMQNLFSKNLIVLNYNATSKEDVIRKMASMFNENGYLNDMEAFIKEIKKREETNGTGIEEHIAMPHAKGNFIKKHGIA
ILRVVGNGFDFNSSDQKLSKLFFMMALPEETPSNAHIKAISYLSNTFSNNLLRHELMSTNNEDRFLEIILNNDNINESNN
LNTKKDFILAVTACPVGIAHTYMAAESLKKAALELNINIKVETNGSSGTENPITEEEIKKAKGVIIASGKTIDKERFSGK
PLIEVGVKDGIHKAKELIQTILKNEAPIYKKSNTNKTTETLQKQNKKTGIYKHLMNGVSFMLPFVVSGGIIIAISFMFGI
KAFDINDPSYNKIADILMQIGGGSAFALMIPILAGYISFSIAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLT
VKKISDKIIPSNLRGINPVLTYPFLSVIISGILIYGMLSPISVINESITNMLNQLSGTNMAILGALLGGMMAIDMGGPVN
KAAYAFGIAMITAKNYIPHASIMAGGMIPPIGIALATSLFKNRFSKEERESGKVCYFLGACFITEGVIPFAAADPLRVIP
ACILGSSVGGFISALFKVEVIAPHGGIFILPIVVNPLMWITSILVGSIITAVLIGILKKEYKNIND
>Mature_626_residues
MIIMQNLFSKNLIVLNYNATSKEDVIRKMASMFNENGYLNDMEAFIKEIKKREETNGTGIEEHIAMPHAKGNFIKKHGIA
ILRVVGNGFDFNSSDQKLSKLFFMMALPEETPSNAHIKAISYLSNTFSNNLLRHELMSTNNEDRFLEIILNNDNINESNN
LNTKKDFILAVTACPVGIAHTYMAAESLKKAALELNINIKVETNGSSGTENPITEEEIKKAKGVIIASGKTIDKERFSGK
PLIEVGVKDGIHKAKELIQTILKNEAPIYKKSNTNKTTETLQKQNKKTGIYKHLMNGVSFMLPFVVSGGIIIAISFMFGI
KAFDINDPSYNKIADILMQIGGGSAFALMIPILAGYISFSIAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLT
VKKISDKIIPSNLRGINPVLTYPFLSVIISGILIYGMLSPISVINESITNMLNQLSGTNMAILGALLGGMMAIDMGGPVN
KAAYAFGIAMITAKNYIPHASIMAGGMIPPIGIALATSLFKNRFSKEERESGKVCYFLGACFITEGVIPFAAADPLRVIP
ACILGSSVGGFISALFKVEVIAPHGGIFILPIVVNPLMWITSILVGSIITAVLIGILKKEYKNIND

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=458, Percent_Identity=44.5414847161572, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1786951, Length=641, Percent_Identity=32.2932917316693, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI87082348, Length=469, Percent_Identity=32.8358208955224, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1790386, Length=313, Percent_Identity=41.5335463258786, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1788729, Length=383, Percent_Identity=28.1984334203655, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=45.2631578947368, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788730, Length=95, Percent_Identity=37.8947368421053, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790390, Length=72, Percent_Identity=43.0555555555556, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 67806; Mature: 67806

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIMQNLFSKNLIVLNYNATSKEDVIRKMASMFNENGYLNDMEAFIKEIKKREETNGTGI
CEEEHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
EEHIAMPHAKGNFIKKHGIAILRVVGNGFDFNSSDQKLSKLFFMMALPEETPSNAHIKAI
HHHHCCCCCCCCCCHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
SYLSNTFSNNLLRHELMSTNNEDRFLEIILNNDNINESNNLNTKKDFILAVTACPVGIAH
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHEEEEEEECCCHHHH
TYMAAESLKKAALELNINIKVETNGSSGTENPITEEEIKKAKGVIIASGKTIDKERFSGK
HHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCHHHHHHHCCEEEECCCCCCHHHCCCC
PLIEVGVKDGIHKAKELIQTILKNEAPIYKKSNTNKTTETLQKQNKKTGIYKHLMNGVSF
CEEEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
MLPFVVSGGIIIAISFMFGIKAFDINDPSYNKIADILMQIGGGSAFALMIPILAGYISFS
HHHHHHHCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLTVKKISDKIIPSNLRGINPVL
HHCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCHHEEEEEHHHCCCCCCCCCCCCHHH
TYPFLSVIISGILIYGMLSPISVINESITNMLNQLSGTNMAILGALLGGMMAIDMGGPVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCC
KAAYAFGIAMITAKNYIPHASIMAGGMIPPIGIALATSLFKNRFSKEERESGKVCYFLGA
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEHHH
CFITEGVIPFAAADPLRVIPACILGSSVGGFISALFKVEVIAPHGGIFILPIVVNPLMWI
HHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHH
TSILVGSIITAVLIGILKKEYKNIND
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIIMQNLFSKNLIVLNYNATSKEDVIRKMASMFNENGYLNDMEAFIKEIKKREETNGTGI
CEEEHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
EEHIAMPHAKGNFIKKHGIAILRVVGNGFDFNSSDQKLSKLFFMMALPEETPSNAHIKAI
HHHHCCCCCCCCCCHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
SYLSNTFSNNLLRHELMSTNNEDRFLEIILNNDNINESNNLNTKKDFILAVTACPVGIAH
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHEEEEEEECCCHHHH
TYMAAESLKKAALELNINIKVETNGSSGTENPITEEEIKKAKGVIIASGKTIDKERFSGK
HHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCHHHHHHHCCEEEECCCCCCHHHCCCC
PLIEVGVKDGIHKAKELIQTILKNEAPIYKKSNTNKTTETLQKQNKKTGIYKHLMNGVSF
CEEEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
MLPFVVSGGIIIAISFMFGIKAFDINDPSYNKIADILMQIGGGSAFALMIPILAGYISFS
HHHHHHHCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLTVKKISDKIIPSNLRGINPVL
HHCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCHHEEEEEHHHCCCCCCCCCCCCHHH
TYPFLSVIISGILIYGMLSPISVINESITNMLNQLSGTNMAILGALLGGMMAIDMGGPVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCC
KAAYAFGIAMITAKNYIPHASIMAGGMIPPIGIALATSLFKNRFSKEERESGKVCYFLGA
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEHHH
CFITEGVIPFAAADPLRVIPACILGSSVGGFISALFKVEVIAPHGGIFILPIVVNPLMWI
HHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHH
TSILVGSIITAVLIGILKKEYKNIND
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]