Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 15594753

GI number: 15594753

Start: 419503

End: 421380

Strand: Reverse

Name: fruA [H]

Synonym: BB0408

Alternate gene names: 15594753

Gene position: 421380-419503 (Counterclockwise)

Preceding gene: 15594758

Following gene: 15594752

Centisome position: 46.27

GC content: 35.62

Gene sequence:

>1878_bases
ATGTTTTTTAATTTTTTGAAAAAAGATCTTGTATTTGTTTTGCCAGAAGTAAATTCAAAAGAAGATGTAATTGATTTTTT
AATTGAAAAAATCAATGATAAGGGATATATAGATAATAAAAAAGAGTTTCTTCAAGGAATTCTTGATAGAGAAAAGATAG
GTGACACTTCTTGGGAAAATGGGGTTGCAATTCCTCATTTTATAGGAGATGTTGTTAAGACTAGTTTTATTTCATTGCTT
TACATTAAAGGTTCTGGGGTTAAGTGGTCTGAAGAAAACCCCCCTGTTAATTTAATATTTTTGATTTGTATGTCAAAAAA
ACAACAAGGTAATGAACACCTTAAGGCGATTGCTTTTATAGCTAAACTATTTGAAGATGATGCTTTTCAAAATGCTTTAC
GCGGGTTTGTTACTACTGATGACATTTATTATTATATTGAAAATGTTCAAAGAAAGGCTAAAGAAGAGGTTTTTGGAGCT
ACAAAAGCAGAAAAAATAGTGGCCGTAACTGCTTGTCCTGTTGGAGTTGCTCATACGTATATTGCAGCTAAGAAAATTGA
AAATGAAGCTAAAAAGCAGGGTTATAGCATTAGAGTAGAAACTCAAGGATCTATTGGTATTGAAAATGCCTTAACAGAAG
AGGAAATTAAGAATGCGTCCGTTGTAATACTTGCTGTTGATAAGGATATTGATGAAAAGAGATTTGAAGGTAAGAGAGTT
TATAAAGTTTCAACTGTAAAAGCGATAAACAATACAGAAAATATTATTAAGGAATCTTTTAATGCTCCGGTATTTAAAAG
CAAAGACTCCGGTACTAATAGTGATACTAAAGCTTCGGTTGCAACAGGCAAAGGTAGTTTTTATAAATATTTAATGAGTG
GGGTATCTCCAATGATTCCTGTTGTTGCAAGTGGAGGAATTTTAATTGCTCTTAGCATTGCTTTTGTTGGGATTGGACCT
GATGGGCCTAATTTTGCTGAGCATCCATTTTATAAGCAGATTGCAGATATTGGTTCTATAGCTTTTGGGATGATGTTGCC
CGTGCTTGCTGGTTTTATTGCAATGGCAATTGCTGATAAGCCTGGTCTTACCCCCGGTCTTGTTGGTGGAGTAATGTCTG
GGAATGTAAAAGCAGGTTTCTTGGGCGCAATATTTGCGGGCTTTCTTGCAGGTTATGTTGCAAGGTTTTTAGCAAGAAGA
TCTGTTCCTGAGTGGTTAAGACCTGTAATGCCTATATTTGTAATTCCGCTAATAAGCACCATTATTGTCGGCTTTTTTAT
GCTGTATTTTGGTGTTTATATTGGAAAATTTATGGGGGTGCTTGAGAGTGGGCTTAAATCTTTACAGAGTAATTCGGAAA
CTTTTGGCGTGTTGGGTAAAATTTTCTTAGGCTTAGTACTAGGTTCAATGATTACTGTTGATATGGGCGGACCTTTTAAT
AAAGTGGCATTTCTTTTTGGTGTAGGGCTAATTCCTCAAGTGCCAGAAATAATGGGAATGGTAGCAGCAGCAATTCCTGT
TCCTCCTATGGCTATGGGGCTTGCAACCTTTTTAGCACCTAAATTGTTTGAAAATGAAGAAAAAGAATCTGGTAAAATAG
CCTTTTTAATTTCATTTATTGGTATTAGCGAAGGAGCTATTCCTTTTGCTGCTAGTGATCCCGGACGGGTAATCCCTTCG
ATAGTGGTAGGGGGAGCTGTATCAAGCATTATTGCCGCTTTTTTAGGCGTTGCTAATCATGCTCCACACGGAGGACCAAT
AGTACTTCCTGTTATTGATAATAAATTTGGGTTTATTATTGCAATTGCTGTTGGAGTTGCGGTTGCAACAGCTTTGGTAA
TTTTTTTGAAATCTTTAAAATTAAAGGAATCTGAATGA

Upstream 100 bases:

>100_bases
CCCTGTTTAGTTTAAAAAATATTTTTTATATTAGGTGGCTGTGGTATGCTATTTTTATTGTATATTATACAATATGTTTA
ATTACTAGGAGACCACAGTT

Downstream 100 bases:

>100_bases
ATAATGAAGATAATATTTTTTTAATGAAAAATAATATTAAAGAATATGATTGGGGCGGAATTAATTTTATTCCCAATCTT
TTGGGTGATAAGATTGATGG

Product: PTS system, fructose-specific IIABC component (fruA-1)

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MFFNFLKKDLVFVLPEVNSKEDVIDFLIEKINDKGYIDNKKEFLQGILDREKIGDTSWENGVAIPHFIGDVVKTSFISLL
YIKGSGVKWSEENPPVNLIFLICMSKKQQGNEHLKAIAFIAKLFEDDAFQNALRGFVTTDDIYYYIENVQRKAKEEVFGA
TKAEKIVAVTACPVGVAHTYIAAKKIENEAKKQGYSIRVETQGSIGIENALTEEEIKNASVVILAVDKDIDEKRFEGKRV
YKVSTVKAINNTENIIKESFNAPVFKSKDSGTNSDTKASVATGKGSFYKYLMSGVSPMIPVVASGGILIALSIAFVGIGP
DGPNFAEHPFYKQIADIGSIAFGMMLPVLAGFIAMAIADKPGLTPGLVGGVMSGNVKAGFLGAIFAGFLAGYVARFLARR
SVPEWLRPVMPIFVIPLISTIIVGFFMLYFGVYIGKFMGVLESGLKSLQSNSETFGVLGKIFLGLVLGSMITVDMGGPFN
KVAFLFGVGLIPQVPEIMGMVAAAIPVPPMAMGLATFLAPKLFENEEKESGKIAFLISFIGISEGAIPFAASDPGRVIPS
IVVGGAVSSIIAAFLGVANHAPHGGPIVLPVIDNKFGFIIAIAVGVAVATALVIFLKSLKLKESE

Sequences:

>Translated_625_residues
MFFNFLKKDLVFVLPEVNSKEDVIDFLIEKINDKGYIDNKKEFLQGILDREKIGDTSWENGVAIPHFIGDVVKTSFISLL
YIKGSGVKWSEENPPVNLIFLICMSKKQQGNEHLKAIAFIAKLFEDDAFQNALRGFVTTDDIYYYIENVQRKAKEEVFGA
TKAEKIVAVTACPVGVAHTYIAAKKIENEAKKQGYSIRVETQGSIGIENALTEEEIKNASVVILAVDKDIDEKRFEGKRV
YKVSTVKAINNTENIIKESFNAPVFKSKDSGTNSDTKASVATGKGSFYKYLMSGVSPMIPVVASGGILIALSIAFVGIGP
DGPNFAEHPFYKQIADIGSIAFGMMLPVLAGFIAMAIADKPGLTPGLVGGVMSGNVKAGFLGAIFAGFLAGYVARFLARR
SVPEWLRPVMPIFVIPLISTIIVGFFMLYFGVYIGKFMGVLESGLKSLQSNSETFGVLGKIFLGLVLGSMITVDMGGPFN
KVAFLFGVGLIPQVPEIMGMVAAAIPVPPMAMGLATFLAPKLFENEEKESGKIAFLISFIGISEGAIPFAASDPGRVIPS
IVVGGAVSSIIAAFLGVANHAPHGGPIVLPVIDNKFGFIIAIAVGVAVATALVIFLKSLKLKESE
>Mature_625_residues
MFFNFLKKDLVFVLPEVNSKEDVIDFLIEKINDKGYIDNKKEFLQGILDREKIGDTSWENGVAIPHFIGDVVKTSFISLL
YIKGSGVKWSEENPPVNLIFLICMSKKQQGNEHLKAIAFIAKLFEDDAFQNALRGFVTTDDIYYYIENVQRKAKEEVFGA
TKAEKIVAVTACPVGVAHTYIAAKKIENEAKKQGYSIRVETQGSIGIENALTEEEIKNASVVILAVDKDIDEKRFEGKRV
YKVSTVKAINNTENIIKESFNAPVFKSKDSGTNSDTKASVATGKGSFYKYLMSGVSPMIPVVASGGILIALSIAFVGIGP
DGPNFAEHPFYKQIADIGSIAFGMMLPVLAGFIAMAIADKPGLTPGLVGGVMSGNVKAGFLGAIFAGFLAGYVARFLARR
SVPEWLRPVMPIFVIPLISTIIVGFFMLYFGVYIGKFMGVLESGLKSLQSNSETFGVLGKIFLGLVLGSMITVDMGGPFN
KVAFLFGVGLIPQVPEIMGMVAAAIPVPPMAMGLATFLAPKLFENEEKESGKIAFLISFIGISEGAIPFAASDPGRVIPS
IVVGGAVSSIIAAFLGVANHAPHGGPIVLPVIDNKFGFIIAIAVGVAVATALVIFLKSLKLKESE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=468, Percent_Identity=43.1623931623932, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1786951, Length=633, Percent_Identity=27.8041074249605, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082348, Length=475, Percent_Identity=33.0526315789474, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790386, Length=339, Percent_Identity=41.0029498525074, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788729, Length=397, Percent_Identity=27.7078085642317, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790387, Length=93, Percent_Identity=50.5376344086022, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788730, Length=98, Percent_Identity=42.8571428571429, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790390, Length=102, Percent_Identity=35.2941176470588, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 67131; Mature: 67131

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNFLKKDLVFVLPEVNSKEDVIDFLIEKINDKGYIDNKKEFLQGILDREKIGDTSWEN
CCHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GVAIPHFIGDVVKTSFISLLYIKGSGVKWSEENPPVNLIFLICMSKKQQGNEHLKAIAFI
CCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
AKLFEDDAFQNALRGFVTTDDIYYYIENVQRKAKEEVFGATKAEKIVAVTACPVGVAHTY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHH
IAAKKIENEAKKQGYSIRVETQGSIGIENALTEEEIKNASVVILAVDKDIDEKRFEGKRV
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCHHHHCCCEEEEEEECCCCCHHHCCCCEE
YKVSTVKAINNTENIIKESFNAPVFKSKDSGTNSDTKASVATGKGSFYKYLMSGVSPMIP
EEEEHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCHHHH
VVASGGILIALSIAFVGIGPDGPNFAEHPFYKQIADIGSIAFGMMLPVLAGFIAMAIADK
HHHCCCHHEEEEEHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGLTPGLVGGVMSGNVKAGFLGAIFAGFLAGYVARFLARRSVPEWLRPVMPIFVIPLIST
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIVGFFMLYFGVYIGKFMGVLESGLKSLQSNSETFGVLGKIFLGLVLGSMITVDMGGPFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
KVAFLFGVGLIPQVPEIMGMVAAAIPVPPMAMGLATFLAPKLFENEEKESGKIAFLISFI
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
GISEGAIPFAASDPGRVIPSIVVGGAVSSIIAAFLGVANHAPHGGPIVLPVIDNKFGFII
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHH
AIAVGVAVATALVIFLKSLKLKESE
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFFNFLKKDLVFVLPEVNSKEDVIDFLIEKINDKGYIDNKKEFLQGILDREKIGDTSWEN
CCHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GVAIPHFIGDVVKTSFISLLYIKGSGVKWSEENPPVNLIFLICMSKKQQGNEHLKAIAFI
CCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
AKLFEDDAFQNALRGFVTTDDIYYYIENVQRKAKEEVFGATKAEKIVAVTACPVGVAHTY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHH
IAAKKIENEAKKQGYSIRVETQGSIGIENALTEEEIKNASVVILAVDKDIDEKRFEGKRV
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCHHHHCCCEEEEEEECCCCCHHHCCCCEE
YKVSTVKAINNTENIIKESFNAPVFKSKDSGTNSDTKASVATGKGSFYKYLMSGVSPMIP
EEEEHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCHHHH
VVASGGILIALSIAFVGIGPDGPNFAEHPFYKQIADIGSIAFGMMLPVLAGFIAMAIADK
HHHCCCHHEEEEEHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGLTPGLVGGVMSGNVKAGFLGAIFAGFLAGYVARFLARRSVPEWLRPVMPIFVIPLIST
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIVGFFMLYFGVYIGKFMGVLESGLKSLQSNSETFGVLGKIFLGLVLGSMITVDMGGPFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
KVAFLFGVGLIPQVPEIMGMVAAAIPVPPMAMGLATFLAPKLFENEEKESGKIAFLISFI
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
GISEGAIPFAASDPGRVIPSIVVGGAVSSIIAAFLGVANHAPHGGPIVLPVIDNKFGFII
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHH
AIAVGVAVATALVIFLKSLKLKESE
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]