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The map label for this gene is clpA [C]

Identifier: 15594714

GI number: 15594714

Start: 377039

End: 379330

Strand: Reverse

Name: clpA [C]

Synonym: BB0369

Alternate gene names: 15594714

Gene position: 379330-377039 (Counterclockwise)

Preceding gene: 15594715

Following gene: 15594713

Centisome position: 41.65

GC content: 27.44

Gene sequence:

>2292_bases
ATGTATGATAGAAGAGCTTTAAATTGTTTATTTTTTAACACCTTTTTGTTTTTCATGGAATCTAGGCACCTTGTGTTTAC
AGAAGAGCACATTTTTTATGGGCTTATTAAAAGTGATAAAGTTAAAGAACTACTTAATTTGTGTGCAATTGATTTTTATA
AACTTAATAAACAACTAGAAGAATTTTTTAGTAAACTTCCCTTAAGAGGCAATTATATCCCAGACTATGTTTCTAGTATG
GATTATTTGTATGACGATATAATTAGTGTTCTTTTTTTTTATAAAAAACCTTATAAAATACAAGAAAAAGATCTATTGTG
GGTGCTTGTCAAAAAAAGAAAAAATAGTATTTTAGATGCGCTGCTTAGCTCGGGTTTTAATTTGACTATTTTTGATAAAC
TTATTGAAGCTCATGATTATTTAGCTGTAAATACTAAATCTGCCTCAGGCGACAGTGAATTAATTGCAGAATATATTCAT
AATAATGCGCCAAAAAGGAAAGGAGGCTTTCATATTTTTGATGATAAGCGTGATGAATTGGATCAAAATAATATTTTCTT
AGAAAGTAAAGACTCTATTGGTAATTTTTTAACAAACGTTATTGATACTTTGGATTTAAAATACAATCCTTTAATTGGTA
GAAGTCAAGAATTATCTCGGTTAATCCAGGTGATACTTAGGAAGCATAAAAGTAATCCTATTTTGTTTGGAGAGCCTGGT
GTTGGAAAAACAGTATTAATCCAAGGTCTTGCATATAAAATAAAAATAGAGAATGTTCCAAAGGATTTAATAGGGTATGA
AATCTATTCTCTTGATATTGGTAGGCTTGTTTCGGGTACTAAATATAGGGGAGATCTTGAGAGTAGGGTGAATAGGGTTT
TAGATTTTTTAAGCTCAAGAAAAAAAGTTATGCTTTTTATTGATGAAATCCATATGATAGTAGGGGCAGGGGCTACTTCA
TTTGGCAGTATGGATATTTCCAATTTGTTAAAACCCATTCTCACTTTAGGAAAAATTAAATTTATTGGAGCTACTACAGA
ATATGAATATAGAAAATTTTTTTTAAAAGATAAGGCCTTAATGAGAAGGTTTCAGAGTATAGAGCTCAAAGAGCCTAATT
TTGAAGACGCTTATAATATTTTGCAGGAGATTAAAAAAGATTACGAGAGGTATCATAATGTGGAATATACAGACGAGGCA
ATACAAGCTTGCATTCTCATGTCTCAAAAATATATTAAAGATAGATTTCTTCCAGACAAAGCTTTTGATATTTTAGATGA
ATTAGGCTCTAAGTTTAAGCTTGAAAATATAAAAAGGATTATAACAAAAGATGATGTTTGCGATCTGATTAAATCTATTG
TTGGTTCTAATATTTTTAATTTTGAAGAGTATGATGGTGAATTGCTAATTAATTTAGAAAATAGAATAAAAAAAGAACTT
ATTATACATGATAACTTGGTACTTGATTTGATATTAAATATTAAATTATTAAAATTCAATTTGCTTGCCAATAGAAGTAC
TATTGGAATATTTGCCTTTATTGGTGCTTCTGGGTCAGGAAAATGCAAATTGATGGATATTTTATCAGAAGAGTTTAAAA
TTCCTAAATTTAGTCTTAACATGGGTGAGTATAATGATTTTAATTCTCTTGATAGATTGATTGGGCCTGTTTTAAGTAAT
GAAGGATATTATGAATCTACCAGATTTTTTAAATTTTTGAACAAATCTTCTAATTCTATTATTTTCCTATCAGATTTTGA
TAAATGTAATAAAAGGGTTTTAGATTTTTTTTTAGAGGGGTTTAAAACAGGTAAACTTTTTGATGGTCTTGGAAAAAAGG
TAAGCTTATCAGAAAGTTTAATAGTAATAAGTATCAATGCTGAGAGCAAAGAGCTTAATAGCATTGGTTTTAGAAATAAA
ATGGCGGGGGAAAATGATTTTAACTTTATATTAAAGAAGAGATTGCCCAATGAGTTTTTAGAATTAATAGATCATGTGTT
TGTATTTAAATCTATTGATGAGTTAGATTTTGAAAAAATCATTTTTAATGAACTTAATTATTTTGCAAGGATATTAAGAG
ATAGAAAATTTGATGTTTTTTTTGAAAAAAGTGTTGTTGATTATATTCGAGAAAAGATTTATGGAAAGGGGTACAGCTTA
AAAAGTGTTAGAAAATTTATATTCAAAGAATTGGGAAAGCTTTTAATAGATGAAATTCTTTTTAAGAAAATTGAAAATTC
TGGTAAAATAAAAATCTATTTAGATGAAACAATAAAATATGAGTTTTTATAA

Upstream 100 bases:

>100_bases
CAGAGTCACCTTCTTACCAAAAAGGATTTTAATAACAATGAAGTTGAATTAAGAGTAGGTAAAAAAAAATTTTTACGAAT
TGTTATATAGTTGATTTTGG

Downstream 100 bases:

>100_bases
GGTTATAGGGGGAGTATTTATGAAAATATCAGTAATAGGGGCAGGTGCTTGGGGAACAGCTATTTCAAAGTCTTTGGCAG
ATAAATTTGATTTTAATATT

Product: ATP-dependent Clp protease subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MYDRRALNCLFFNTFLFFMESRHLVFTEEHIFYGLIKSDKVKELLNLCAIDFYKLNKQLEEFFSKLPLRGNYIPDYVSSM
DYLYDDIISVLFFYKKPYKIQEKDLLWVLVKKRKNSILDALLSSGFNLTIFDKLIEAHDYLAVNTKSASGDSELIAEYIH
NNAPKRKGGFHIFDDKRDELDQNNIFLESKDSIGNFLTNVIDTLDLKYNPLIGRSQELSRLIQVILRKHKSNPILFGEPG
VGKTVLIQGLAYKIKIENVPKDLIGYEIYSLDIGRLVSGTKYRGDLESRVNRVLDFLSSRKKVMLFIDEIHMIVGAGATS
FGSMDISNLLKPILTLGKIKFIGATTEYEYRKFFLKDKALMRRFQSIELKEPNFEDAYNILQEIKKDYERYHNVEYTDEA
IQACILMSQKYIKDRFLPDKAFDILDELGSKFKLENIKRIITKDDVCDLIKSIVGSNIFNFEEYDGELLINLENRIKKEL
IIHDNLVLDLILNIKLLKFNLLANRSTIGIFAFIGASGSGKCKLMDILSEEFKIPKFSLNMGEYNDFNSLDRLIGPVLSN
EGYYESTRFFKFLNKSSNSIIFLSDFDKCNKRVLDFFLEGFKTGKLFDGLGKKVSLSESLIVISINAESKELNSIGFRNK
MAGENDFNFILKKRLPNEFLELIDHVFVFKSIDELDFEKIIFNELNYFARILRDRKFDVFFEKSVVDYIREKIYGKGYSL
KSVRKFIFKELGKLLIDEILFKKIENSGKIKIYLDETIKYEFL

Sequences:

>Translated_763_residues
MYDRRALNCLFFNTFLFFMESRHLVFTEEHIFYGLIKSDKVKELLNLCAIDFYKLNKQLEEFFSKLPLRGNYIPDYVSSM
DYLYDDIISVLFFYKKPYKIQEKDLLWVLVKKRKNSILDALLSSGFNLTIFDKLIEAHDYLAVNTKSASGDSELIAEYIH
NNAPKRKGGFHIFDDKRDELDQNNIFLESKDSIGNFLTNVIDTLDLKYNPLIGRSQELSRLIQVILRKHKSNPILFGEPG
VGKTVLIQGLAYKIKIENVPKDLIGYEIYSLDIGRLVSGTKYRGDLESRVNRVLDFLSSRKKVMLFIDEIHMIVGAGATS
FGSMDISNLLKPILTLGKIKFIGATTEYEYRKFFLKDKALMRRFQSIELKEPNFEDAYNILQEIKKDYERYHNVEYTDEA
IQACILMSQKYIKDRFLPDKAFDILDELGSKFKLENIKRIITKDDVCDLIKSIVGSNIFNFEEYDGELLINLENRIKKEL
IIHDNLVLDLILNIKLLKFNLLANRSTIGIFAFIGASGSGKCKLMDILSEEFKIPKFSLNMGEYNDFNSLDRLIGPVLSN
EGYYESTRFFKFLNKSSNSIIFLSDFDKCNKRVLDFFLEGFKTGKLFDGLGKKVSLSESLIVISINAESKELNSIGFRNK
MAGENDFNFILKKRLPNEFLELIDHVFVFKSIDELDFEKIIFNELNYFARILRDRKFDVFFEKSVVDYIREKIYGKGYSL
KSVRKFIFKELGKLLIDEILFKKIENSGKIKIYLDETIKYEFL
>Mature_763_residues
MYDRRALNCLFFNTFLFFMESRHLVFTEEHIFYGLIKSDKVKELLNLCAIDFYKLNKQLEEFFSKLPLRGNYIPDYVSSM
DYLYDDIISVLFFYKKPYKIQEKDLLWVLVKKRKNSILDALLSSGFNLTIFDKLIEAHDYLAVNTKSASGDSELIAEYIH
NNAPKRKGGFHIFDDKRDELDQNNIFLESKDSIGNFLTNVIDTLDLKYNPLIGRSQELSRLIQVILRKHKSNPILFGEPG
VGKTVLIQGLAYKIKIENVPKDLIGYEIYSLDIGRLVSGTKYRGDLESRVNRVLDFLSSRKKVMLFIDEIHMIVGAGATS
FGSMDISNLLKPILTLGKIKFIGATTEYEYRKFFLKDKALMRRFQSIELKEPNFEDAYNILQEIKKDYERYHNVEYTDEA
IQACILMSQKYIKDRFLPDKAFDILDELGSKFKLENIKRIITKDDVCDLIKSIVGSNIFNFEEYDGELLINLENRIKKEL
IIHDNLVLDLILNIKLLKFNLLANRSTIGIFAFIGASGSGKCKLMDILSEEFKIPKFSLNMGEYNDFNSLDRLIGPVLSN
EGYYESTRFFKFLNKSSNSIIFLSDFDKCNKRVLDFFLEGFKTGKLFDGLGKKVSLSESLIVISINAESKELNSIGFRNK
MAGENDFNFILKKRLPNEFLELIDHVFVFKSIDELDFEKIIFNELNYFARILRDRKFDVFFEKSVVDYIREKIYGKGYSL
KSVRKFIFKELGKLLIDEILFKKIENSGKIKIYLDETIKYEFL

Specific function: ATP-Dependent Specificity Component Of The Clpp Protease. It Directs The Protease To Specific Substrates. The Primary Function Of The Clpa-Clpp Complex Appears To Be The Degradation Of Unfolded Or Abnormal Proteins. [C]

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=302, Percent_Identity=21.8543046357616, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787109, Length=567, Percent_Identity=33.1569664902998, Blast_Score=321, Evalue=1e-88,
Organism=Escherichia coli, GI1788943, Length=413, Percent_Identity=35.8353510895884, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320464, Length=249, Percent_Identity=45.3815261044177, Blast_Score=210, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6323002, Length=221, Percent_Identity=43.4389140271493, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR013461
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 88907; Mature: 88907

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDRRALNCLFFNTFLFFMESRHLVFTEEHIFYGLIKSDKVKELLNLCAIDFYKLNKQLE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EFFSKLPLRGNYIPDYVSSMDYLYDDIISVLFFYKKPYKIQEKDLLWVLVKKRKNSILDA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSSGFNLTIFDKLIEAHDYLAVNTKSASGDSELIAEYIHNNAPKRKGGFHIFDDKRDEL
HHHCCCCEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHCC
DQNNIFLESKDSIGNFLTNVIDTLDLKYNPLIGRSQELSRLIQVILRKHKSNPILFGEPG
CCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCC
VGKTVLIQGLAYKIKIENVPKDLIGYEIYSLDIGRLVSGTKYRGDLESRVNRVLDFLSSR
CCHHHEEECEEEEEEECCCCHHHHCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCC
KKVMLFIDEIHMIVGAGATSFGSMDISNLLKPILTLGKIKFIGATTEYEYRKFFLKDKAL
CEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHH
MRRFQSIELKEPNFEDAYNILQEIKKDYERYHNVEYTDEAIQACILMSQKYIKDRFLPDK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
AFDILDELGSKFKLENIKRIITKDDVCDLIKSIVGSNIFNFEEYDGELLINLENRIKKEL
HHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHH
IIHDNLVLDLILNIKLLKFNLLANRSTIGIFAFIGASGSGKCKLMDILSEEFKIPKFSLN
HHCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEEECCCCCCEEHHHHHHHHHCCCEEEEC
MGEYNDFNSLDRLIGPVLSNEGYYESTRFFKFLNKSSNSIIFLSDFDKCNKRVLDFFLEG
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHC
FKTGKLFDGLGKKVSLSESLIVISINAESKELNSIGFRNKMAGENDFNFILKKRLPNEFL
CCCCCHHHHCCCCEECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHH
ELIDHVFVFKSIDELDFEKIIFNELNYFARILRDRKFDVFFEKSVVDYIREKIYGKGYSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCH
KSVRKFIFKELGKLLIDEILFKKIENSGKIKIYLDETIKYEFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECC
>Mature Secondary Structure
MYDRRALNCLFFNTFLFFMESRHLVFTEEHIFYGLIKSDKVKELLNLCAIDFYKLNKQLE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EFFSKLPLRGNYIPDYVSSMDYLYDDIISVLFFYKKPYKIQEKDLLWVLVKKRKNSILDA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSSGFNLTIFDKLIEAHDYLAVNTKSASGDSELIAEYIHNNAPKRKGGFHIFDDKRDEL
HHHCCCCEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHCC
DQNNIFLESKDSIGNFLTNVIDTLDLKYNPLIGRSQELSRLIQVILRKHKSNPILFGEPG
CCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCC
VGKTVLIQGLAYKIKIENVPKDLIGYEIYSLDIGRLVSGTKYRGDLESRVNRVLDFLSSR
CCHHHEEECEEEEEEECCCCHHHHCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCC
KKVMLFIDEIHMIVGAGATSFGSMDISNLLKPILTLGKIKFIGATTEYEYRKFFLKDKAL
CEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHH
MRRFQSIELKEPNFEDAYNILQEIKKDYERYHNVEYTDEAIQACILMSQKYIKDRFLPDK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
AFDILDELGSKFKLENIKRIITKDDVCDLIKSIVGSNIFNFEEYDGELLINLENRIKKEL
HHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHH
IIHDNLVLDLILNIKLLKFNLLANRSTIGIFAFIGASGSGKCKLMDILSEEFKIPKFSLN
HHCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEEECCCCCCEEHHHHHHHHHCCCEEEEC
MGEYNDFNSLDRLIGPVLSNEGYYESTRFFKFLNKSSNSIIFLSDFDKCNKRVLDFFLEG
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHC
FKTGKLFDGLGKKVSLSESLIVISINAESKELNSIGFRNKMAGENDFNFILKKRLPNEFL
CCCCCHHHHCCCCEECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHH
ELIDHVFVFKSIDELDFEKIIFNELNYFARILRDRKFDVFFEKSVVDYIREKIYGKGYSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCH
KSVRKFIFKELGKLLIDEILFKKIENSGKIKIYLDETIKYEFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6209404 [H]