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The map label for this gene is tyrS [H]

Identifier: 15594715

GI number: 15594715

Start: 379341

End: 380558

Strand: Reverse

Name: tyrS [H]

Synonym: BB0370

Alternate gene names: 15594715

Gene position: 380558-379341 (Counterclockwise)

Preceding gene: 15594716

Following gene: 15594714

Centisome position: 41.79

GC content: 30.05

Gene sequence:

>1218_bases
ATGAATCTTGCTTTAAGTCTTTTACATAAACGCGGATTTTTAAAGCAATGTACATCTTTAAAAGTTTTAAGTGATTTAAT
GGATAGGGAAAAAATAGTTTTTTATGCAGGAGTTGATGCAACATCTAGTTCTCTTCATATTGGCCATTTGATTCCCTTTT
TAGCAATGATGCATCTTAGGCAACACGGGCACATGCCAATTGTTTTGATTGGAGATTCTACAGCAAAAATAGGCGATCCT
TCTGGAAAAAGTGAGATGAGAAAGATTTTATCTTCAGAAGAGATTGGCAATAATGCTTTGTTGATAAAAAATCAACTTCA
AAGAATAACTAAGTTTACTTCAGAATGTTTTATTCATAATTCAAATTGGTTAGATAATCTCAATTATATTGAATTTTTAA
GAGATGTTGGCATGCATTTTTCTGTTAATCGTATGTTAAGCTTTGAAACTTATAAAAGAAGGATGGATTTCGGACTTTCA
TTTATTGAGTTTAATTATCAACTTTTGCAGTCTTATGATTATTATATGCTTAATAAAATTAAAAATTGCCGACTTCAAAT
TGGTGGTGATGATCAATGGGGGAATATTATTTCAGGGGTTGACCTAATTAGAAAAAAAAATGGATCAGAAACTTTTGGGC
TTACTTTTCCATTAATTACAAGAAGTGATGGAAAAAAGATGGGTAAATCAGAAAAAGGCGCTGTTTATCTTGATTCTAAT
CTTTTTAGTATTTATGATTTTTATCAGTATTTTAGAAATACTTCAGATTCTGATGTGAAAACTTTTTTATATCTTTTTAC
TTTTTTAGAAGAAGATGAGATTGAATTAATTTCAAATTTTAAGGGGAATTCTTTAAATAAGGCCAAAGAGATTTTGGCTT
TTGAGATAACTAAAATTGTTCACGGAGAGGCAGAAGCCTTGAAAGTTCAAGAGGCATCTTTTGCCGCATTTAGGGGAAGT
GGAGATAGGAGCAATATTCCATTTTTTAAATTTAGCTTTTCTAGCCTAAAAGAAGAGATATTATTGGTTGATTTAATGTT
AGATTCAAAAATTGTGCCCAGCAAATCAGAAGGCAGAAGATTGATTGATTCTGGAGGTGTTTATATTAATGGTAAAAGGG
TAGAAAGTCAGAGTCACCTTCTTACCAAAAAGGATTTTAATAACAATGAAGTTGAATTAAGAGTAGGTAAAAAAAAATTT
TTACGAATTGTTATATAG

Upstream 100 bases:

>100_bases
TTAGAGAAAGAAATAGCATGACTCAGAAGAGAATTTTTATTAATGATTTATATTCATACATTAAAACAGAGATTTTAAAT
TACAAAGAGGATTTTAATAA

Downstream 100 bases:

>100_bases
TTGATTTTGGATGTATGATAGAAGAGCTTTAAATTGTTTATTTTTTAACACCTTTTTGTTTTTCATGGAATCTAGGCACC
TTGTGTTTACAGAAGAGCAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNLALSLLHKRGFLKQCTSLKVLSDLMDREKIVFYAGVDATSSSLHIGHLIPFLAMMHLRQHGHMPIVLIGDSTAKIGDP
SGKSEMRKILSSEEIGNNALLIKNQLQRITKFTSECFIHNSNWLDNLNYIEFLRDVGMHFSVNRMLSFETYKRRMDFGLS
FIEFNYQLLQSYDYYMLNKIKNCRLQIGGDDQWGNIISGVDLIRKKNGSETFGLTFPLITRSDGKKMGKSEKGAVYLDSN
LFSIYDFYQYFRNTSDSDVKTFLYLFTFLEEDEIELISNFKGNSLNKAKEILAFEITKIVHGEAEALKVQEASFAAFRGS
GDRSNIPFFKFSFSSLKEEILLVDLMLDSKIVPSKSEGRRLIDSGGVYINGKRVESQSHLLTKKDFNNNEVELRVGKKKF
LRIVI

Sequences:

>Translated_405_residues
MNLALSLLHKRGFLKQCTSLKVLSDLMDREKIVFYAGVDATSSSLHIGHLIPFLAMMHLRQHGHMPIVLIGDSTAKIGDP
SGKSEMRKILSSEEIGNNALLIKNQLQRITKFTSECFIHNSNWLDNLNYIEFLRDVGMHFSVNRMLSFETYKRRMDFGLS
FIEFNYQLLQSYDYYMLNKIKNCRLQIGGDDQWGNIISGVDLIRKKNGSETFGLTFPLITRSDGKKMGKSEKGAVYLDSN
LFSIYDFYQYFRNTSDSDVKTFLYLFTFLEEDEIELISNFKGNSLNKAKEILAFEITKIVHGEAEALKVQEASFAAFRGS
GDRSNIPFFKFSFSSLKEEILLVDLMLDSKIVPSKSEGRRLIDSGGVYINGKRVESQSHLLTKKDFNNNEVELRVGKKKF
LRIVI
>Mature_405_residues
MNLALSLLHKRGFLKQCTSLKVLSDLMDREKIVFYAGVDATSSSLHIGHLIPFLAMMHLRQHGHMPIVLIGDSTAKIGDP
SGKSEMRKILSSEEIGNNALLIKNQLQRITKFTSECFIHNSNWLDNLNYIEFLRDVGMHFSVNRMLSFETYKRRMDFGLS
FIEFNYQLLQSYDYYMLNKIKNCRLQIGGDDQWGNIISGVDLIRKKNGSETFGLTFPLITRSDGKKMGKSEKGAVYLDSN
LFSIYDFYQYFRNTSDSDVKTFLYLFTFLEEDEIELISNFKGNSLNKAKEILAFEITKIVHGEAEALKVQEASFAAFRGS
GDRSNIPFFKFSFSSLKEEILLVDLMLDSKIVPSKSEGRRLIDSGGVYINGKRVESQSHLLTKKDFNNNEVELRVGKKKF
LRIVI

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=412, Percent_Identity=33.7378640776699, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1787925, Length=414, Percent_Identity=37.9227053140097, Blast_Score=288, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI25145802, Length=361, Percent_Identity=35.4570637119114, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25145805, Length=273, Percent_Identity=34.0659340659341, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325160, Length=431, Percent_Identity=34.338747099768, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI20130383, Length=428, Percent_Identity=33.6448598130841, Blast_Score=241, Evalue=7e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46364; Mature: 46364

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLALSLLHKRGFLKQCTSLKVLSDLMDREKIVFYAGVDATSSSLHIGHLIPFLAMMHLR
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHH
QHGHMPIVLIGDSTAKIGDPSGKSEMRKILSSEEIGNNALLIKNQLQRITKFTSECFIHN
HCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEC
SNWLDNLNYIEFLRDVGMHFSVNRMLSFETYKRRMDFGLSFIEFNYQLLQSYDYYMLNKI
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHH
KNCRLQIGGDDQWGNIISGVDLIRKKNGSETFGLTFPLITRSDGKKMGKSEKGAVYLDSN
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHCCCCCCCEEEEECC
LFSIYDFYQYFRNTSDSDVKTFLYLFTFLEEDEIELISNFKGNSLNKAKEILAFEITKIV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HGEAEALKVQEASFAAFRGSGDRSNIPFFKFSFSSLKEEILLVDLMLDSKIVPSKSEGRR
CCCHHHEEEEHHHHHEEECCCCCCCCCEEEEEHHHHHHHHEEEEEEECCCCCCCCCCCCE
LIDSGGVYINGKRVESQSHLLTKKDFNNNEVELRVGKKKFLRIVI
EEECCCEEEECEEECCHHHEEEECCCCCCEEEEEECCCCEEEECC
>Mature Secondary Structure
MNLALSLLHKRGFLKQCTSLKVLSDLMDREKIVFYAGVDATSSSLHIGHLIPFLAMMHLR
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHH
QHGHMPIVLIGDSTAKIGDPSGKSEMRKILSSEEIGNNALLIKNQLQRITKFTSECFIHN
HCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHEEEC
SNWLDNLNYIEFLRDVGMHFSVNRMLSFETYKRRMDFGLSFIEFNYQLLQSYDYYMLNKI
CCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHH
KNCRLQIGGDDQWGNIISGVDLIRKKNGSETFGLTFPLITRSDGKKMGKSEKGAVYLDSN
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHCCCCCCCEEEEECC
LFSIYDFYQYFRNTSDSDVKTFLYLFTFLEEDEIELISNFKGNSLNKAKEILAFEITKIV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HGEAEALKVQEASFAAFRGSGDRSNIPFFKFSFSSLKEEILLVDLMLDSKIVPSKSEGRR
CCCHHHEEEEHHHHHEEECCCCCCCCCEEEEEHHHHHHHHEEEEEEECCCCCCCCCCCCE
LIDSGGVYINGKRVESQSHLLTKKDFNNNEVELRVGKKKFLRIVI
EEECCCEEEECEEECCHHHEEEECCCCCCEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA