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The map label for this gene is gatB [H]

Identifier: 15594686

GI number: 15594686

Start: 348153

End: 349610

Strand: Reverse

Name: gatB [H]

Synonym: BB0341

Alternate gene names: 15594686

Gene position: 349610-348153 (Counterclockwise)

Preceding gene: 15594687

Following gene: 15594685

Centisome position: 38.39

GC content: 32.58

Gene sequence:

>1458_bases
ATGGAATATAAATTAGTTATTGGATTAGAAATTCATGTTCAACTGGGTTTAAAAACAAAGGCTTTTTGTGGATGTAAAAA
TGAGTTTGGAGGAGTTCCCAACTCTCGTGTTTGTCCAATTTGTCTTGGATTGCCAGGTTCATTACCAAGTGTGAATGTAG
AGCTTATTAATAGTGCAATTTTAGCGGGGCATGCCACAAATTCAAAGATTAGAAATGTTGTTAAATTTGATAGAAAGCAT
TATTATTATCCAGATTTGCCAAAAGGATATCAAATCTCGCAAAATGATAAGCCAATTTGTGAGGGGGGAAGCTTATTGAT
TGAAACCCCTTCTGGACCCAAAAAGATTAACATTATTAGAATTCATATGGAAGAAGATTCTGGCAAGAGTCTACATTTAC
TGGACAGTGAAAATCAAAGTTATGTTGATTTTAATCGCTCGGGTGCTCCTTTGCTTGAGATTGTTTCTGCTCCAGATATT
AACAGTGGAGATGAAGCAGTTGCTTTCCTAAGCTCTTTAAGAGAAATTTTTAGGTATCTTGATTTGTCCGAATGTAATAT
GGAGAATGGTTCTTTTAGATGCGATGTAAATGTTAATTTAATTGTTAAAGAGAATGGTGTTGAACATAAAACTCCTATAG
CTGAAATAAAGAATTTAAATTCTTTTAAATCTATTAAAGCGGCCATTGAATATGAAGAATTAAGGCAGCAACAGGAGTGG
ATTCAATTTAAGAAAACTCTTAATAGTTGTGGTAAGCACACTAGAGGATTTGATGATAGGAGCGGAGTAACGGTTATTCA
AAGAAATAAAGAGACAGTATCTGATTATCGCTATTTTCAAGAACCCGACCTGCCTTTAATAGAGATTGATGATTCTTATA
TTGATAATATTAAAAATTTAAAGTTGATTGAACTTCCATTTCATGCAAGAATTAGGCTTAAGGGCCAATATGGGCTAAGT
GATTTTGATGTTATTACTTTAACAGCAGATAAGCATCTGCTTAAATATTTTGAAGAGGCTGTTATTAATTCAAGCGATCC
CAAAAAAGTAGCCAATTGGATATTGTCTGAAGTTTTAAGCGTTCTTAATGATAAAGGAATTAGTGTTCTTGAATTTAATT
TGCTTCCAAGCTATATTACAGAGCTTGTTGAATTTATTGTTGCTGGCAAAATAAGTGGCAAAATGGCAAAAAGGGTATTT
TCAGAGATGATGACTAGAGGAGTTTCTGCCTCTGTTGTTATAAGTGAAAATCAATTAGAGCAAGTAAGTGATAAGTTTGT
TATTAAGCAGATTGTGCTTGAAGTTTTAGATGAAAATCCTAAATCAATTGAACTTTACAAAAAGGGCAAAGACCATGCTA
TCAAATTTATGATGGGGCAAATAATGAAAAAATCTTCAGGAAAGATTAATCCTATACTTGCAAATGAAATTCTTTTAGAA
AGTTTATCAAATGTATGA

Upstream 100 bases:

>100_bases
GAGGAAGGATTGTCAATTGGGATGCAAATTATTGGGCGTAGCAAAAAGGATTTTGAACTTTTAAGTTTTTCAAAAAATGT
GATTAGGGAATTAGGATTGA

Downstream 100 bases:

>100_bases
TTTGCCTTTAATAGATAATTTACCAGTGATTAAAAGGGCAAGATTTTTTTATCTTTACGATATTCATGGTAAGAGGTATT
TGGATTTATATTTAAATGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEYKLVIGLEIHVQLGLKTKAFCGCKNEFGGVPNSRVCPICLGLPGSLPSVNVELINSAILAGHATNSKIRNVVKFDRKH
YYYPDLPKGYQISQNDKPICEGGSLLIETPSGPKKINIIRIHMEEDSGKSLHLLDSENQSYVDFNRSGAPLLEIVSAPDI
NSGDEAVAFLSSLREIFRYLDLSECNMENGSFRCDVNVNLIVKENGVEHKTPIAEIKNLNSFKSIKAAIEYEELRQQQEW
IQFKKTLNSCGKHTRGFDDRSGVTVIQRNKETVSDYRYFQEPDLPLIEIDDSYIDNIKNLKLIELPFHARIRLKGQYGLS
DFDVITLTADKHLLKYFEEAVINSSDPKKVANWILSEVLSVLNDKGISVLEFNLLPSYITELVEFIVAGKISGKMAKRVF
SEMMTRGVSASVVISENQLEQVSDKFVIKQIVLEVLDENPKSIELYKKGKDHAIKFMMGQIMKKSSGKINPILANEILLE
SLSNV

Sequences:

>Translated_485_residues
MEYKLVIGLEIHVQLGLKTKAFCGCKNEFGGVPNSRVCPICLGLPGSLPSVNVELINSAILAGHATNSKIRNVVKFDRKH
YYYPDLPKGYQISQNDKPICEGGSLLIETPSGPKKINIIRIHMEEDSGKSLHLLDSENQSYVDFNRSGAPLLEIVSAPDI
NSGDEAVAFLSSLREIFRYLDLSECNMENGSFRCDVNVNLIVKENGVEHKTPIAEIKNLNSFKSIKAAIEYEELRQQQEW
IQFKKTLNSCGKHTRGFDDRSGVTVIQRNKETVSDYRYFQEPDLPLIEIDDSYIDNIKNLKLIELPFHARIRLKGQYGLS
DFDVITLTADKHLLKYFEEAVINSSDPKKVANWILSEVLSVLNDKGISVLEFNLLPSYITELVEFIVAGKISGKMAKRVF
SEMMTRGVSASVVISENQLEQVSDKFVIKQIVLEVLDENPKSIELYKKGKDHAIKFMMGQIMKKSSGKINPILANEILLE
SLSNV
>Mature_485_residues
MEYKLVIGLEIHVQLGLKTKAFCGCKNEFGGVPNSRVCPICLGLPGSLPSVNVELINSAILAGHATNSKIRNVVKFDRKH
YYYPDLPKGYQISQNDKPICEGGSLLIETPSGPKKINIIRIHMEEDSGKSLHLLDSENQSYVDFNRSGAPLLEIVSAPDI
NSGDEAVAFLSSLREIFRYLDLSECNMENGSFRCDVNVNLIVKENGVEHKTPIAEIKNLNSFKSIKAAIEYEELRQQQEW
IQFKKTLNSCGKHTRGFDDRSGVTVIQRNKETVSDYRYFQEPDLPLIEIDDSYIDNIKNLKLIELPFHARIRLKGQYGLS
DFDVITLTADKHLLKYFEEAVINSSDPKKVANWILSEVLSVLNDKGISVLEFNLLPSYITELVEFIVAGKISGKMAKRVF
SEMMTRGVSASVVISENQLEQVSDKFVIKQIVLEVLDENPKSIELYKKGKDHAIKFMMGQIMKKSSGKINPILANEILLE
SLSNV

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=504, Percent_Identity=34.7222222222222, Blast_Score=274, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=306, Percent_Identity=35.6209150326797, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=30.1343570057582, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=31.211498973306, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54521; Mature: 54521

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKLVIGLEIHVQLGLKTKAFCGCKNEFGGVPNSRVCPICLGLPGSLPSVNVELINSAI
CCEEEEEEEEEEEEECCCHHHHCCCCHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
LAGHATNSKIRNVVKFDRKHYYYPDLPKGYQISQNDKPICEGGSLLIETPSGPKKINIIR
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCEEEEEE
IHMEEDSGKSLHLLDSENQSYVDFNRSGAPLLEIVSAPDINSGDEAVAFLSSLREIFRYL
EEEECCCCCEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHC
DLSECNMENGSFRCDVNVNLIVKENGVEHKTPIAEIKNLNSFKSIKAAIEYEELRQQQEW
CHHHCCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IQFKKTLNSCGKHTRGFDDRSGVTVIQRNKETVSDYRYFQEPDLPLIEIDDSYIDNIKNL
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
KLIELPFHARIRLKGQYGLSDFDVITLTADKHLLKYFEEAVINSSDPKKVANWILSEVLS
EEEEECCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLNDKGISVLEFNLLPSYITELVEFIVAGKISGKMAKRVFSEMMTRGVSASVVISENQLE
HHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHH
QVSDKFVIKQIVLEVLDENPKSIELYKKGKDHAIKFMMGQIMKKSSGKINPILANEILLE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLSNV
HHHCC
>Mature Secondary Structure
MEYKLVIGLEIHVQLGLKTKAFCGCKNEFGGVPNSRVCPICLGLPGSLPSVNVELINSAI
CCEEEEEEEEEEEEECCCHHHHCCCCHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
LAGHATNSKIRNVVKFDRKHYYYPDLPKGYQISQNDKPICEGGSLLIETPSGPKKINIIR
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCEEEEEE
IHMEEDSGKSLHLLDSENQSYVDFNRSGAPLLEIVSAPDINSGDEAVAFLSSLREIFRYL
EEEECCCCCEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHC
DLSECNMENGSFRCDVNVNLIVKENGVEHKTPIAEIKNLNSFKSIKAAIEYEELRQQQEW
CHHHCCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IQFKKTLNSCGKHTRGFDDRSGVTVIQRNKETVSDYRYFQEPDLPLIEIDDSYIDNIKNL
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
KLIELPFHARIRLKGQYGLSDFDVITLTADKHLLKYFEEAVINSSDPKKVANWILSEVLS
EEEEECCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLNDKGISVLEFNLLPSYITELVEFIVAGKISGKMAKRVFSEMMTRGVSASVVISENQLE
HHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHH
QVSDKFVIKQIVLEVLDENPKSIELYKKGKDHAIKFMMGQIMKKSSGKINPILANEILLE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLSNV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA