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The map label for this gene is gatA

Identifier: 15594687

GI number: 15594687

Start: 349600

End: 351090

Strand: Reverse

Name: gatA

Synonym: BB0342

Alternate gene names: 15594687

Gene position: 351090-349600 (Counterclockwise)

Preceding gene: 15594688

Following gene: 15594686

Centisome position: 38.55

GC content: 30.11

Gene sequence:

>1491_bases
ATGGATATTTTTCATCTCCTAAAATATTGGAGTAAGGATAAGGTGTTGGACTTAAGTAATTTAACTTTAACCAAAATTCA
AGAATTAGTTTTAACTAGAAAATGTAAAATTTATGATATTTTGCTTGCTTATAAAAATAATTATGAGCTAAATAAAGATA
TCAATGGATATATTGAATTTTTTGATGATTCTTTAGAGATTGCAAAAAGGTATGACGATTGTTTAAAAAATTGTGAATTA
GAAGATTTGCCTTTAATTGGTATGCTTATTGCAGTCAAAGATAATATTTCAATTCAAGATAAATCTTTAACTTGTGCTTC
TGAGATTTTAAAAGGTTATATTTCTCCTTATGATGCGACTGTTATTAAAAGGCTTAAGAATAAAGGAGCAATTTTAATTG
GTAGAACCAATATGGATGAATTTGCCATGGGTTCTACTTGTGAATTTTCTTATTACGGTGCAACTTTAAATCCTTTAAAT
AGAGAATATGTTATAGGTGGTAGTTCTGGAGGCTCTGCAGCTGTAGTTGCAGCTTTTCAAGCACCTTTTTCGCTTGGTAG
TGATACTGGAGGTTCTGTTAGGCTGCCCGCATCTTTTTCAGGAATTTTGGGTTTTAAACCTTCTTATGGAGGTCTTTCTC
GCTATGGGCTTGCATCTTATGCTTCGTCTTTTGATCAAATAGGATTTTTTTCTCATTCTATTGAAGATATTGCTTTAATT
TTAAAGCATACTTGTGGATCTGATAAAATGGATTCTACTAGTGTAGACATTTTTGATGATTTTTATCCTTTAAAAATTGA
GTCGTTGCAAGGTAAAAATTTAGCTGTAATCAAAGAGCTTAGCGAAGATCTAATGGACAAAAATGTTGCAAATAGTTTTG
CAAAGTTTAAATTAGACCTTTTGTCAAAGGGTATTAATATAAAAGAAGTTTCAATAGAAGAGATTAATTTTATTTTATCA
ATTTATTATATAATTTCTCCTGTTGAAGCATCCTCCAATCTTGCTCGTTATACTGGACTTTGTTACGGCAAGAGAATCTC
TGAAGGTTTGAGTCTTAATGATTTTTATTTTAAACATAGGAGCAATTTCTTGTCAGAAGAAGTTAAAAGGCGTATTGTTC
TTGGAAATTATTTGTTATCAGAAAGGTATGATTCTAAATATTATGCAAAAGCTTGTGAAATTCTTCAAAATTTGATTATT
CCTAAATTTAACAAGCTTTTTGAAAGCTGTGATTTTATTATTACCCCAACAAGCTTTGTTAAACCTTTTAGACTTGGTTT
GGATTTTGATGATCCTGTTAAAATGTATTATTCAGATATTTGTACTGTTATTGCAAATCTTATTGGAGCCCCTGCTATTT
CGCTTCCATATTCTAAGGATGAGGAAGGATTGTCAATTGGGATGCAAATTATTGGGCGTAGCAAAAAGGATTTTGAACTT
TTAAGTTTTTCAAAAAATGTGATTAGGGAATTAGGATTGAATGGAATATAA

Upstream 100 bases:

>100_bases
TTTAATGCTAATAAGAAAAAGATTTCTACGTTGCGCGAGGATAAAGTAGAATTTTCTCTTTCTATTGAAGCAATTAAAAA
ACTTAGTAATTCGTTTTTAG

Downstream 100 bases:

>100_bases
ATTAGTTATTGGATTAGAAATTCATGTTCAACTGGGTTTAAAAACAAAGGCTTTTTGTGGATGTAAAAATGAGTTTGGAG
GAGTTCCCAACTCTCGTGTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MDIFHLLKYWSKDKVLDLSNLTLTKIQELVLTRKCKIYDILLAYKNNYELNKDINGYIEFFDDSLEIAKRYDDCLKNCEL
EDLPLIGMLIAVKDNISIQDKSLTCASEILKGYISPYDATVIKRLKNKGAILIGRTNMDEFAMGSTCEFSYYGATLNPLN
REYVIGGSSGGSAAVVAAFQAPFSLGSDTGGSVRLPASFSGILGFKPSYGGLSRYGLASYASSFDQIGFFSHSIEDIALI
LKHTCGSDKMDSTSVDIFDDFYPLKIESLQGKNLAVIKELSEDLMDKNVANSFAKFKLDLLSKGINIKEVSIEEINFILS
IYYIISPVEASSNLARYTGLCYGKRISEGLSLNDFYFKHRSNFLSEEVKRRIVLGNYLLSERYDSKYYAKACEILQNLII
PKFNKLFESCDFIITPTSFVKPFRLGLDFDDPVKMYYSDICTVIANLIGAPAISLPYSKDEEGLSIGMQIIGRSKKDFEL
LSFSKNVIRELGLNGI

Sequences:

>Translated_496_residues
MDIFHLLKYWSKDKVLDLSNLTLTKIQELVLTRKCKIYDILLAYKNNYELNKDINGYIEFFDDSLEIAKRYDDCLKNCEL
EDLPLIGMLIAVKDNISIQDKSLTCASEILKGYISPYDATVIKRLKNKGAILIGRTNMDEFAMGSTCEFSYYGATLNPLN
REYVIGGSSGGSAAVVAAFQAPFSLGSDTGGSVRLPASFSGILGFKPSYGGLSRYGLASYASSFDQIGFFSHSIEDIALI
LKHTCGSDKMDSTSVDIFDDFYPLKIESLQGKNLAVIKELSEDLMDKNVANSFAKFKLDLLSKGINIKEVSIEEINFILS
IYYIISPVEASSNLARYTGLCYGKRISEGLSLNDFYFKHRSNFLSEEVKRRIVLGNYLLSERYDSKYYAKACEILQNLII
PKFNKLFESCDFIITPTSFVKPFRLGLDFDDPVKMYYSDICTVIANLIGAPAISLPYSKDEEGLSIGMQIIGRSKKDFEL
LSFSKNVIRELGLNGI
>Mature_496_residues
MDIFHLLKYWSKDKVLDLSNLTLTKIQELVLTRKCKIYDILLAYKNNYELNKDINGYIEFFDDSLEIAKRYDDCLKNCEL
EDLPLIGMLIAVKDNISIQDKSLTCASEILKGYISPYDATVIKRLKNKGAILIGRTNMDEFAMGSTCEFSYYGATLNPLN
REYVIGGSSGGSAAVVAAFQAPFSLGSDTGGSVRLPASFSGILGFKPSYGGLSRYGLASYASSFDQIGFFSHSIEDIALI
LKHTCGSDKMDSTSVDIFDDFYPLKIESLQGKNLAVIKELSEDLMDKNVANSFAKFKLDLLSKGINIKEVSIEEINFILS
IYYIISPVEASSNLARYTGLCYGKRISEGLSLNDFYFKHRSNFLSEEVKRRIVLGNYLLSERYDSKYYAKACEILQNLII
PKFNKLFESCDFIITPTSFVKPFRLGLDFDDPVKMYYSDICTVIANLIGAPAISLPYSKDEEGLSIGMQIIGRSKKDFEL
LSFSKNVIRELGLNGI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=463, Percent_Identity=36.0691144708423, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=25.1968503937008, Blast_Score=119, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=427, Percent_Identity=35.5971896955504, Blast_Score=234, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17537465, Length=466, Percent_Identity=24.8927038626609, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=268, Percent_Identity=29.1044776119403, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=166, Percent_Identity=33.7349397590361, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=180, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=158, Percent_Identity=27.8481012658228, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=37.5870069605568, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=29.0094339622642, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=197, Percent_Identity=26.9035532994924, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=420, Percent_Identity=35.2380952380952, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550774, Length=516, Percent_Identity=24.6124031007752, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=516, Percent_Identity=24.6124031007752, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648437, Length=516, Percent_Identity=24.6124031007752, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652985, Length=466, Percent_Identity=27.0386266094421, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922090, Length=466, Percent_Identity=27.0386266094421, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652981, Length=466, Percent_Identity=27.0386266094421, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652983, Length=466, Percent_Identity=27.0386266094421, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24644968, Length=469, Percent_Identity=22.8144989339019, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=441, Percent_Identity=24.7165532879819, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648439, Length=441, Percent_Identity=24.7165532879819, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_BORBU (O51317)

Other databases:

- EMBL:   AE000783
- PIR:   E70142
- RefSeq:   NP_212476.1
- ProteinModelPortal:   O51317
- SMR:   O51317
- EnsemblBacteria:   EBBORT00000009009
- GeneID:   1195179
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0342
- NMPDR:   fig|224326.1.peg.726
- TIGR:   BB_0342
- GeneTree:   EBGT00050000007061
- HOGENOM:   HBG481888
- OMA:   ICTVIAN
- PhylomeDB:   O51317
- ProtClustDB:   PRK00012
- BioCyc:   BBUR224326:BB_0342-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 55546; Mature: 55546

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 93-93 ACT_SITE 168-168 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFHLLKYWSKDKVLDLSNLTLTKIQELVLTRKCKIYDILLAYKNNYELNKDINGYIEF
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEECCCCCHHHHH
FDDSLEIAKRYDDCLKNCELEDLPLIGMLIAVKDNISIQDKSLTCASEILKGYISPYDAT
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHCCCCHHH
VIKRLKNKGAILIGRTNMDEFAMGSTCEFSYYGATLNPLNREYVIGGSSGGSAAVVAAFQ
HHHHHHCCCCEEEEECCCHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEC
APFSLGSDTGGSVRLPASFSGILGFKPSYGGLSRYGLASYASSFDQIGFFSHSIEDIALI
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHTCGSDKMDSTSVDIFDDFYPLKIESLQGKNLAVIKELSEDLMDKNVANSFAKFKLDL
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKGINIKEVSIEEINFILSIYYIISPVEASSNLARYTGLCYGKRISEGLSLNDFYFKHR
HHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
SNFLSEEVKRRIVLGNYLLSERYDSKYYAKACEILQNLIIPKFNKLFESCDFIITPTSFV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCHHHH
KPFRLGLDFDDPVKMYYSDICTVIANLIGAPAISLPYSKDEEGLSIGMQIIGRSKKDFEL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCCCCHHH
LSFSKNVIRELGLNGI
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDIFHLLKYWSKDKVLDLSNLTLTKIQELVLTRKCKIYDILLAYKNNYELNKDINGYIEF
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEECCCCCHHHHH
FDDSLEIAKRYDDCLKNCELEDLPLIGMLIAVKDNISIQDKSLTCASEILKGYISPYDAT
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHCCCCHHH
VIKRLKNKGAILIGRTNMDEFAMGSTCEFSYYGATLNPLNREYVIGGSSGGSAAVVAAFQ
HHHHHHCCCCEEEEECCCHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEC
APFSLGSDTGGSVRLPASFSGILGFKPSYGGLSRYGLASYASSFDQIGFFSHSIEDIALI
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKHTCGSDKMDSTSVDIFDDFYPLKIESLQGKNLAVIKELSEDLMDKNVANSFAKFKLDL
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKGINIKEVSIEEINFILSIYYIISPVEASSNLARYTGLCYGKRISEGLSLNDFYFKHR
HHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
SNFLSEEVKRRIVLGNYLLSERYDSKYYAKACEILQNLIIPKFNKLFESCDFIITPTSFV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCHHHH
KPFRLGLDFDDPVKMYYSDICTVIANLIGAPAISLPYSKDEEGLSIGMQIIGRSKKDFEL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCCCCHHH
LSFSKNVIRELGLNGI
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685