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The map label for this gene is fliM

Identifier: 15594623

GI number: 15594623

Start: 289963

End: 291021

Strand: Reverse

Name: fliM

Synonym: BB0278

Alternate gene names: 15594623

Gene position: 291021-289963 (Counterclockwise)

Preceding gene: 15594624

Following gene: 15594622

Centisome position: 31.95

GC content: 33.52

Gene sequence:

>1059_bases
ATGGCAAACAATCCAGGAGCTTTATCTCAAGATGATATAGATAGTCTTTTAGAGTCTATCAACTCGTCTGAAAGTTTATC
CTTAGACGAATCCCTTTCTAATGTTATATCTAGCCCTACAGGCAAAAAGCAAAAAGTTAAGGTTTATGACTTTAAAAGGC
CAGATAAATTTTCAAAAGAGCAGGTTAGAACAGTTTCAAGTTTTCACGAGGCATTTGCCAGATATACTACAACTTCACTT
TCTGCTCTTTTAAGAAAAATGGTTCATGTTCACGTAGCCTCAGTTGATCAATTAACCTATGAAGAGTTTATTAGGTCAAT
ACCCAACCCTACAACTTTAGCAATAATAAATATGGACCCTCTTAAAGGATCTGCTATTTTTGAAGTTGATCCAACAATAG
CATTTGCTATTGTAGATAGGCTTTTTGGGGGTGATGGAGATACGATTAAAGATAAAAGTAGAGATTTAACAGAAATTGAG
CAGTCTGTTATGGAAAGTGTTATTATTCGTATACTTGCTAATATGAGAGAGGCTTGGTCTCAAGTTGTTGATTTAAGGCC
AAGATTTGGTCATATTGAGGTCAATCCACAGTTTGCTCAAATAGTTCCCCCTACAGAAATGATTATTTTAGTAACTCTTG
AGGTTAAAATAGGAAAAGTAGAAGGGCTTATGAATTTTTGTTTGCCTTATATTACCATAGAGCCTATTGTTTCAAAATTA
TCAACAAGATATTGGCATTCTTTGATTGGGGTTGGAACTACTAGCGAGAATCTTGATGCTTTGCGTGAAAAGTTGGAAAA
TACAGCGATGCCTTTGGTAGCAGAAATAGGGGAAGTTAAACTTAAAGTAAGAGAAATTTTATCGCTTGACAAAGGTGATG
TTTTAAATCTTGAATCTTCTCTAATAAATAAAGATTTAACTTTGAAAGTTGGTACCAAAGAAAAGTTTAAATGCAGAATG
GGTTTAATGGGAAATAAAGTTTCAGTGCAAATTACAGAAAAAATTGGAGATATAAAAGGTTTTGATTTATTAAAAGAGCT
TACAGAAGAGGTTGAATGA

Upstream 100 bases:

>100_bases
ATTCTTAGAAATGGAGAAATAAAAGAAATAGCATTAACCCAAATTGATATTTTTGATATGTAATAAATTTAAAAGAATTT
TAAATTTAAAGGAATTTTAA

Downstream 100 bases:

>100_bases
TAGTTGTTTTATTTATTAAAACTTAAATTATTAAGAGGTTGAATTATGAGTGTAGATGAAAAAAGTGATAATGGAGAAAA
GCCGGAAATAAAGGGGGTTA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MANNPGALSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSL
SALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIE
QSVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
STRYWHSLIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLTLKVGTKEKFKCRM
GLMGNKVSVQITEKIGDIKGFDLLKELTEEVE

Sequences:

>Translated_352_residues
MANNPGALSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSL
SALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIE
QSVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
STRYWHSLIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLTLKVGTKEKFKCRM
GLMGNKVSVQITEKIGDIKGFDLLKELTEEVE
>Mature_351_residues
ANNPGALSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSSFHEAFARYTTTSLS
ALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQ
SVMESVIIRILANMREAWSQVVDLRPRFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLS
TRYWHSLIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLTLKVGTKEKFKCRMG
LMGNKVSVQITEKIGDIKGFDLLKELTEEVE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family

Homologues:

Organism=Escherichia coli, GI1788255, Length=294, Percent_Identity=29.5918367346939, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIM_BORBU (Q57511)

Other databases:

- EMBL:   U43739
- EMBL:   L75945
- EMBL:   AE000783
- PIR:   F70134
- RefSeq:   NP_212412.1
- ProteinModelPortal:   Q57511
- SMR:   Q57511
- EnsemblBacteria:   EBBORT00000009143
- GeneID:   1195115
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0278
- TIGR:   BB_0278
- GeneTree:   EBGT00050000007087
- HOGENOM:   HBG579155
- OMA:   QTEQRII
- PhylomeDB:   Q57511
- ProtClustDB:   PRK06666
- BioCyc:   BBUR224326:BB_0278-MONOMER
- GO:   GO:0006935
- InterPro:   IPR001689
- InterPro:   IPR001543
- PRINTS:   PR00955
- TIGRFAMs:   TIGR01397

Pfam domain/function: PF02154 FliM; PF01052 SpoA; SSF101801 SpoA

EC number: NA

Molecular weight: Translated: 39285; Mature: 39154

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNPGALSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKE
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHH
QVRTVSSFHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
LKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVDLRPRFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
HHHHCCCCCCEEEECCCHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STRYWHSLIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHH
LINKDLTLKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE
HHCCCCEEEECCCHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ANNPGALSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKE
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHH
QVRTVSSFHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
LKGSAIFEVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVDLRPRFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKL
HHHHCCCCCCEEEECCCHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
STRYWHSLIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHH
LINKDLTLKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE
HHCCCCEEEECCCHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9403685