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The map label for this gene is glpF [H]

Identifier: 15594585

GI number: 15594585

Start: 245790

End: 246554

Strand: Direct

Name: glpF [H]

Synonym: BB0240

Alternate gene names: 15594585

Gene position: 245790-246554 (Clockwise)

Preceding gene: 15594582

Following gene: 15594586

Centisome position: 26.99

GC content: 34.25

Gene sequence:

>765_bases
ATGAATTATACAAAATTCCAAGAATTTATATCGGAATTTTTGGGAACATTTATCCTATTGGCTCTAGGAACTGGATCTGT
TGCAATGACAGTATTATTTTCCTCAAGTCCCGAAATACCAGGAGAAATAATAAAAGGAGGATATACAAATATAGTATTTG
GATGGGGATTGGGTGTAACGTTTGGTATTTACACAGCAGCAAGAATGAGCGGAGCACACCTAAACCCAGCTGTTAGCATA
GGATTAGCAAGTGTTGGAAAGTTTCCCGTTTCAAAACTTTTACATTACATTGTAGCACAAATATTAGGAGCTTTTACAGG
TGCATTAATGACACTTGTCGTATTTTATCCTAAATGGATAGAAATGGATCCTGGCTTAGAAAATACTCAAGGAATAATGG
CAACTTTCCCTGCTGTTCCTGGATTTTTGCCTGGATTTATTGATCAAATTTTTGGAACTTTTTTGCTAATGTTTTTAATT
TCTGTTGTTGGAGATTTTACAAAAAAACACAGCGACAATCCATTTATTCCTTTTATTGTAGGAGCAGTGGTTTTATCAAT
AGGGATAAGTTTCGGAGGAATGAACGGTTATGCTATTAATCCTGCAAGGGATCTGGGACCAAGAATTTTACTCTTATTTG
CTGGATTTAAAAATCACGGATTTAACAATCTAAGTATAGTTATTGTACCAATAATTGGCCCAATAATTGGAGCAATTTTG
GGAGCTACAATTTACGAATTTACACTAAAAAATAACAAAGACTAA

Upstream 100 bases:

>100_bases
AAATTTAATTAAAAGATTTAATATTATTCAGATTAAAAAATCAAAAATTAAAACTTCTTAATAAAAAATAAAATAGTTAT
AAAGATAAGGAGATATAATT

Downstream 100 bases:

>100_bases
AAGAAAAAATATTTATCTTTATTAAAGGAAAAAGGAAAAACTATGAAATACATTTTATCTATTGATCAAGGTACTACTAG
CTCGAGAGCAATGGTATTTG

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFSSSPEIPGEIIKGGYTNIVFGWGLGVTFGIYTAARMSGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYPKWIEMDPGLENTQGIMATFPAVPGFLPGFIDQIFGTFLLMFLI
SVVGDFTKKHSDNPFIPFIVGAVVLSIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIVIVPIIGPIIGAIL
GATIYEFTLKNNKD

Sequences:

>Translated_254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFSSSPEIPGEIIKGGYTNIVFGWGLGVTFGIYTAARMSGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYPKWIEMDPGLENTQGIMATFPAVPGFLPGFIDQIFGTFLLMFLI
SVVGDFTKKHSDNPFIPFIVGAVVLSIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIVIVPIIGPIIGAIL
GATIYEFTLKNNKD
>Mature_254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFSSSPEIPGEIIKGGYTNIVFGWGLGVTFGIYTAARMSGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYPKWIEMDPGLENTQGIMATFPAVPGFLPGFIDQIFGTFLLMFLI
SVVGDFTKKHSDNPFIPFIVGAVVLSIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIVIVPIIGPIIGAIL
GATIYEFTLKNNKD

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=257, Percent_Identity=36.5758754863813, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=34.6613545816733, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI22538420, Length=249, Percent_Identity=34.136546184739, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI157266307, Length=254, Percent_Identity=34.6456692913386, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI310133356, Length=212, Percent_Identity=31.6037735849057, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI310114181, Length=212, Percent_Identity=31.6037735849057, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502181, Length=250, Percent_Identity=27.6, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4755125, Length=250, Percent_Identity=27.6, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=34.1269841269841, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71992966, Length=256, Percent_Identity=33.984375, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=35.3413654618474, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=249, Percent_Identity=34.9397590361446, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=249, Percent_Identity=34.9397590361446, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17533613, Length=259, Percent_Identity=33.5907335907336, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=259, Percent_Identity=33.5907335907336, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17544068, Length=210, Percent_Identity=37.6190476190476, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=29.7959183673469, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71993722, Length=252, Percent_Identity=30.5555555555556, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=249, Percent_Identity=25.3012048192771, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=223, Percent_Identity=33.6322869955157, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=27.2413793103448, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24652747, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551084, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27195; Mature: 27195

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFSSSPEIPGEIIKGGYTNIVFGWGLGVT
CCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCHHHHCCCCCEEEEECHHHHH
FGIYTAARMSGAHLNPAVSIGLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYPKWI
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMDPGLENTQGIMATFPAVPGFLPGFIDQIFGTFLLMFLISVVGDFTKKHSDNPFIPFIV
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GAVVLSIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIVIVPIIGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
GATIYEFTLKNNKD
HHHHHHHHCCCCCC
>Mature Secondary Structure
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFSSSPEIPGEIIKGGYTNIVFGWGLGVT
CCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCHHHHCCCCCEEEEECHHHHH
FGIYTAARMSGAHLNPAVSIGLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYPKWI
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMDPGLENTQGIMATFPAVPGFLPGFIDQIFGTFLLMFLISVVGDFTKKHSDNPFIPFIV
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GAVVLSIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIVIVPIIGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
GATIYEFTLKNNKD
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]