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The map label for this gene is glpK [H]

Identifier: 15594586

GI number: 15594586

Start: 246597

End: 248102

Strand: Direct

Name: glpK [H]

Synonym: BB0241

Alternate gene names: 15594586

Gene position: 246597-248102 (Clockwise)

Preceding gene: 15594585

Following gene: 15594587

Centisome position: 27.08

GC content: 36.72

Gene sequence:

>1506_bases
ATGAAATACATTTTATCTATTGATCAAGGTACTACTAGCTCGAGAGCAATGGTATTTGATAAAAATGCAAACATAAAGGG
GTTTGCTCAAAAAGAATTTACTCAAATTTATCCACAACCAAGTTGGGTGGAACATGATCCTACAGAAATATGGGGCTCAC
AACTTGGAGTTATAACAGAAGCTATGGCAAATGCAAGAATTTTGCCCAATGAAATTGACGCTATTGGAATAACTAATCAA
AGAGAAACTACGGTTATATGGGAAAAAAATACAGGAAAGCCCATCTACAATGCAATAGTATGGCAAGACAGAAGAACTGC
AAAAATTTGTGACCAATTAAAAAAAGAAGGAAAAGATAAAATTATTTTGGAAAAAACAGGCTTGGTGCTAGATTCTTATT
TTAGTGGAACAAAAATAATGTGGATATTGGATAATGTAGAAGGTGCCAGACAAAGAGCCGAAAATGGCGAATTATGCTTT
GGAACAATAGATACATGGATATTGTGGAACCTGACTCAAAAAAAAGAACATGCAACCGATTACTCTAATGCTTCAAGAAC
ATTATTATTAAACATTAAAACATTAGAGTGGGATGATGAACTTTTAAGCATATTAAATGTTCCAAGGGCAATTTTGCCTG
AACTTAAAGAAAGTTCTACAATATACGGCAAAACAGACAAAGCACTATTTGGAGCAGAAATTCCTATTGCAGGAATTGCT
GGGGATCAATTTGCAGCAACATTTGGACAAGCCTGCCTTAAAAAGGGTATGGCCAAAAACACTTATGGAACTGGCTGCTT
CTTAACGGTTAATATAGGAAAAGAACCAATCATTAGCCACGACAAGCTTTTAACTTCAATTGCATGGGGAAGAAAAAAAT
CTGTAACCTACGTTCTTGAAGGAAGTGTTTTTATTGGAGGAGCTGTAATCCAGTGGTTAAGAGACGGTCTTGAATTTTTC
AGAAAAAGCTCAGATGCAGAAGCGTTGGCAAGCTCTGTCTCAGATAATGGTGGAGTTTATTTTGTGCCAGCATTTGTTGG
GCTTGGTGCACCTCACTGGGATTCTTATGCAAGAGGAACAATCATCGGAATAACAAGAGGCTCAACAAAAGCTCACATTA
CAAGAGCTGCTCTTGAGAGCATCGCATTTCAAAGCTTCGATATACTAAATACCATGAAAAAATCCATTCCCAACTTTGAA
ATTCAAGAATTAAGAGTAGACGGGGGAGCAAGTCAAAACAATCTATTAATGCAATTTCAAGCTGATCTTTTAGAATGTAA
AGTTGTAAGACCAAAAATAACAGAAACAACCGCTCTTGGCGCTGCTTATCTTGCAGGACTTGCAACGGGTTATTGGCAAA
GCGCCGAAGAAATCGTAAGCCTTTGGCAAGTAGACAAGATATTTGAACCTTCAATGCCAAAAAATCAAAAAGAAAAGCTT
CTTGAGAATTGGAACAAAGCGGTTGGAAAAGCAAAATCCTGGATACAGAATTCTCATAGTTCATAA

Upstream 100 bases:

>100_bases
TGGAGCAATTTTGGGAGCTACAATTTACGAATTTACACTAAAAAATAACAAAGACTAAAAGAAAAAATATTTATCTTTAT
TAAAGGAAAAAGGAAAAACT

Downstream 100 bases:

>100_bases
AAGTTCAAAACAAAGCTTTAAGATTATAACAAAAATATACTGGTAACAAAATTATGCATAATTTATTTGATTTTTTGAAA
CTAAAACAACATATAATAAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKYILSIDQGTTSSRAMVFDKNANIKGFAQKEFTQIYPQPSWVEHDPTEIWGSQLGVITEAMANARILPNEIDAIGITNQ
RETTVIWEKNTGKPIYNAIVWQDRRTAKICDQLKKEGKDKIILEKTGLVLDSYFSGTKIMWILDNVEGARQRAENGELCF
GTIDTWILWNLTQKKEHATDYSNASRTLLLNIKTLEWDDELLSILNVPRAILPELKESSTIYGKTDKALFGAEIPIAGIA
GDQFAATFGQACLKKGMAKNTYGTGCFLTVNIGKEPIISHDKLLTSIAWGRKKSVTYVLEGSVFIGGAVIQWLRDGLEFF
RKSSDAEALASSVSDNGGVYFVPAFVGLGAPHWDSYARGTIIGITRGSTKAHITRAALESIAFQSFDILNTMKKSIPNFE
IQELRVDGGASQNNLLMQFQADLLECKVVRPKITETTALGAAYLAGLATGYWQSAEEIVSLWQVDKIFEPSMPKNQKEKL
LENWNKAVGKAKSWIQNSHSS

Sequences:

>Translated_501_residues
MKYILSIDQGTTSSRAMVFDKNANIKGFAQKEFTQIYPQPSWVEHDPTEIWGSQLGVITEAMANARILPNEIDAIGITNQ
RETTVIWEKNTGKPIYNAIVWQDRRTAKICDQLKKEGKDKIILEKTGLVLDSYFSGTKIMWILDNVEGARQRAENGELCF
GTIDTWILWNLTQKKEHATDYSNASRTLLLNIKTLEWDDELLSILNVPRAILPELKESSTIYGKTDKALFGAEIPIAGIA
GDQFAATFGQACLKKGMAKNTYGTGCFLTVNIGKEPIISHDKLLTSIAWGRKKSVTYVLEGSVFIGGAVIQWLRDGLEFF
RKSSDAEALASSVSDNGGVYFVPAFVGLGAPHWDSYARGTIIGITRGSTKAHITRAALESIAFQSFDILNTMKKSIPNFE
IQELRVDGGASQNNLLMQFQADLLECKVVRPKITETTALGAAYLAGLATGYWQSAEEIVSLWQVDKIFEPSMPKNQKEKL
LENWNKAVGKAKSWIQNSHSS
>Mature_501_residues
MKYILSIDQGTTSSRAMVFDKNANIKGFAQKEFTQIYPQPSWVEHDPTEIWGSQLGVITEAMANARILPNEIDAIGITNQ
RETTVIWEKNTGKPIYNAIVWQDRRTAKICDQLKKEGKDKIILEKTGLVLDSYFSGTKIMWILDNVEGARQRAENGELCF
GTIDTWILWNLTQKKEHATDYSNASRTLLLNIKTLEWDDELLSILNVPRAILPELKESSTIYGKTDKALFGAEIPIAGIA
GDQFAATFGQACLKKGMAKNTYGTGCFLTVNIGKEPIISHDKLLTSIAWGRKKSVTYVLEGSVFIGGAVIQWLRDGLEFF
RKSSDAEALASSVSDNGGVYFVPAFVGLGAPHWDSYARGTIIGITRGSTKAHITRAALESIAFQSFDILNTMKKSIPNFE
IQELRVDGGASQNNLLMQFQADLLECKVVRPKITETTALGAAYLAGLATGYWQSAEEIVSLWQVDKIFEPSMPKNQKEKL
LENWNKAVGKAKSWIQNSHSS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=44.4444444444444, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=44.4444444444444, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI41393575, Length=504, Percent_Identity=43.6507936507937, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI42794763, Length=508, Percent_Identity=43.8976377952756, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=34.251968503937, Blast_Score=329, Evalue=4e-90,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=55.668016194332, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1790005, Length=490, Percent_Identity=24.6938775510204, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787789, Length=484, Percent_Identity=25.6198347107438, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789987, Length=478, Percent_Identity=22.8033472803347, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789138, Length=514, Percent_Identity=23.5408560311284, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789168, Length=505, Percent_Identity=23.960396039604, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=507, Percent_Identity=40.2366863905325, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71986140, Length=516, Percent_Identity=27.7131782945736, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=32.8358208955224, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=559, Percent_Identity=36.3148479427549, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=41.699604743083, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24654573, Length=434, Percent_Identity=43.778801843318, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655555, Length=505, Percent_Identity=39.009900990099, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655560, Length=505, Percent_Identity=39.009900990099, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655570, Length=505, Percent_Identity=39.009900990099, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655574, Length=505, Percent_Identity=39.009900990099, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655565, Length=505, Percent_Identity=39.009900990099, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=35.1043643263757, Blast_Score=301, Evalue=9e-82,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=35.6275303643725, Blast_Score=284, Evalue=8e-77,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=30.2912621359223, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=29.2220113851992, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=31.0541310541311, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI45552909, Length=291, Percent_Identity=29.2096219931272, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55606; Mature: 55606

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYILSIDQGTTSSRAMVFDKNANIKGFAQKEFTQIYPQPSWVEHDPTEIWGSQLGVITE
CCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AMANARILPNEIDAIGITNQRETTVIWEKNTGKPIYNAIVWQDRRTAKICDQLKKEGKDK
HHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHCCCCE
IILEKTGLVLDSYFSGTKIMWILDNVEGARQRAENGELCFGTIDTWILWNLTQKKEHATD
EEEEECCCEEECCCCCCEEEEEEECCHHHHHHCCCCCEEEEEECEEEEEECHHHHHHCCC
YSNASRTLLLNIKTLEWDDELLSILNVPRAILPELKESSTIYGKTDKALFGAEIPIAGIA
CCCCCEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCEEECCCCCEEECCCCCEEECC
GDQFAATFGQACLKKGMAKNTYGTGCFLTVNIGKEPIISHDKLLTSIAWGRKKSVTYVLE
CCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEE
GSVFIGGAVIQWLRDGLEFFRKSSDAEALASSVSDNGGVYFVPAFVGLGAPHWDSYARGT
CCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCCE
IIGITRGSTKAHITRAALESIAFQSFDILNTMKKSIPNFEIQELRVDGGASQNNLLMQFQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEE
ADLLECKVVRPKITETTALGAAYLAGLATGYWQSAEEIVSLWQVDKIFEPSMPKNQKEKL
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
LENWNKAVGKAKSWIQNSHSS
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYILSIDQGTTSSRAMVFDKNANIKGFAQKEFTQIYPQPSWVEHDPTEIWGSQLGVITE
CCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AMANARILPNEIDAIGITNQRETTVIWEKNTGKPIYNAIVWQDRRTAKICDQLKKEGKDK
HHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHCCCCE
IILEKTGLVLDSYFSGTKIMWILDNVEGARQRAENGELCFGTIDTWILWNLTQKKEHATD
EEEEECCCEEECCCCCCEEEEEEECCHHHHHHCCCCCEEEEEECEEEEEECHHHHHHCCC
YSNASRTLLLNIKTLEWDDELLSILNVPRAILPELKESSTIYGKTDKALFGAEIPIAGIA
CCCCCEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCEEECCCCCEEECCCCCEEECC
GDQFAATFGQACLKKGMAKNTYGTGCFLTVNIGKEPIISHDKLLTSIAWGRKKSVTYVLE
CCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEE
GSVFIGGAVIQWLRDGLEFFRKSSDAEALASSVSDNGGVYFVPAFVGLGAPHWDSYARGT
CCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCCE
IIGITRGSTKAHITRAALESIAFQSFDILNTMKKSIPNFEIQELRVDGGASQNNLLMQFQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEE
ADLLECKVVRPKITETTALGAAYLAGLATGYWQSAEEIVSLWQVDKIFEPSMPKNQKEKL
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHH
LENWNKAVGKAKSWIQNSHSS
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA