Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 15594565

GI number: 15594565

Start: 224826

End: 226610

Strand: Reverse

Name: alaS [H]

Synonym: BB0220

Alternate gene names: 15594565

Gene position: 226610-224826 (Counterclockwise)

Preceding gene: 15594566

Following gene: 15594564

Centisome position: 24.88

GC content: 32.83

Gene sequence:

>1785_bases
ATGACACTTAATAAGTTGCGTAAAAAATATATTGATTTTTTTAAATCAAAAAAACATTTTGAAATAATGGGGAAATCATT
AGTTCCTGAAAATGATCCTACAGTTCTTTTTAATACAGCTGGCATGCAGCCCCTTATCCCGTATCTTCTTGGAGAAGTGC
ATCCATCAGGAGATATGCTTGTTAATGTTCAAAAGTGCCTAAGAACAGGCGACATTGATGAGGTTGGAGATTTAAGCCAT
TTAACTTTTTTTGAAATGCTTGGAAATTGGTCTCTTGGAGCTTATTTCAAAGAGTATTCTGTAAAGTGTAGTTTTGAATT
TTTGACATCTTCTGATTATTTAAATATTCCAAAAGACAGTCTTTATGTGAGCGTATTTGAAGGCGATCAAGAGATTCCGC
GCGATACAGAGACTGCTAAAGTTTGGGAAAGCTTGGGTATTTCCAAAGATAGAATACATTATCTTTCTAAGGATCATAAT
TTTTGGGGGCCTGTTGGATCTAAAGGGCCTTGTGGGCCAGATACTGAAATATATGTGGATACTGGAAAGTCTAAGTGTTC
TCTTGACTGTAATATTACGTGTTCTTGTGGTAAGTATTTTGAAATTTGGAATAATGTTTTTATGCAATACAATAAAGATG
AAAATGGTAATTACATAGAATTGGGTCGAAAATGCGTTGATACGGGAATGGGACTTGAGAGGACAATTGCTTTTTTGCAG
GGCAAATCTTCAGTTTATGATACAGATGCATTCATGCCTATAATAAAGAGAATAGAATATATTTCTGGTAAAATTTATGG
ACAAAAAGAAGATGATGATAGATGTATTAGAATAATTTCTGATCATGTTAAAGCTGCTTGCTTTATTCTTGCTGATAGTT
CTGTTGTTTTTCCCTCCAATTTGGGGCAAGGATATGTTTTAAGAAGACTAATAAGGCGATCTATCAGATATGCAAAGAAA
CTTGGGATAAAATCCCATTTTTTAGCTGATCTTGTTGATTCTGTAGAAGCTATTTATAGATCTTTTTATAATGAGCTAAC
AGAAAAAAAAGATTTTATCAAAAAGGAATTAAGCAAAGAAGAAGAAAAGTTTTTTAAAACTTTATCTCAAGGAGAGCAAG
AATTTATTAAAATAACAAGAAATTTGCCTTCCAAGACTATTCCTGGTGATATTGCTTTTAAACTATACGATACTTATGGT
TTTCCATACGAAGTAACAGAAGAGCTTGCTATTGAATATGGCTTTAATGTTGATAAGTTGGGTTTTAATGAGCATTTTAA
AAAGCATCAAAAAACTTCAAAGAAAGGGGGTGACAAAGTTTTTAAAGGAGGGCTTGCCGATTATACTTATGAGACCACTA
AACTACATACAGCGACCCATTTACTGCATAAGGCACTTCAATTAGTACTAGGCGATCATGTTAGACAAAAAGGAAGCAAT
ATTACTGCTGAGCGATTAAGGTTTGATTTTGTTCATTCTGAAAAGATGACGGATGATGAGATAAAAAAAGTTGAAGAGAT
TGTTAATTTACAGATAAAAAATTCTTTGTCTGTAAAAAAAATTATAATGGAATTAAGTGAAGCTCGAGAAAAAGGTGCTA
TGGCTCTTTTTGGAGAAAAATATGACGATTTGGTGAGCGTTTATGAGATAGATGGATTTTCGCTTGAAGTTTGTGGAGGC
CCTCATGTAGAGAATACCAATGAACTTGGTACTTTTAAAATACAAAAAGAACAATCCTCATCTTCAGGTATAAGAAGAAT
AAAAGCTATTTTGATTGATGAGTAA

Upstream 100 bases:

>100_bases
AAGATTTAAAAACTATGACAACAGCTTTTGTTAATTATATTAAAAATTTAACTTTAAAAGGCGATTTAATAATATATAGA
AAAAATGAGGAATTTATTTA

Downstream 100 bases:

>100_bases
ATTACTTATTATTTGTTGATTTGTTTTAATAGGAGGCTTTATGATAAAATCAATTTTAGATTATTTATTAACTTTGCATC
CTGTATTATTGGGACTTTTA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MTLNKLRKKYIDFFKSKKHFEIMGKSLVPENDPTVLFNTAGMQPLIPYLLGEVHPSGDMLVNVQKCLRTGDIDEVGDLSH
LTFFEMLGNWSLGAYFKEYSVKCSFEFLTSSDYLNIPKDSLYVSVFEGDQEIPRDTETAKVWESLGISKDRIHYLSKDHN
FWGPVGSKGPCGPDTEIYVDTGKSKCSLDCNITCSCGKYFEIWNNVFMQYNKDENGNYIELGRKCVDTGMGLERTIAFLQ
GKSSVYDTDAFMPIIKRIEYISGKIYGQKEDDDRCIRIISDHVKAACFILADSSVVFPSNLGQGYVLRRLIRRSIRYAKK
LGIKSHFLADLVDSVEAIYRSFYNELTEKKDFIKKELSKEEEKFFKTLSQGEQEFIKITRNLPSKTIPGDIAFKLYDTYG
FPYEVTEELAIEYGFNVDKLGFNEHFKKHQKTSKKGGDKVFKGGLADYTYETTKLHTATHLLHKALQLVLGDHVRQKGSN
ITAERLRFDFVHSEKMTDDEIKKVEEIVNLQIKNSLSVKKIIMELSEAREKGAMALFGEKYDDLVSVYEIDGFSLEVCGG
PHVENTNELGTFKIQKEQSSSSGIRRIKAILIDE

Sequences:

>Translated_594_residues
MTLNKLRKKYIDFFKSKKHFEIMGKSLVPENDPTVLFNTAGMQPLIPYLLGEVHPSGDMLVNVQKCLRTGDIDEVGDLSH
LTFFEMLGNWSLGAYFKEYSVKCSFEFLTSSDYLNIPKDSLYVSVFEGDQEIPRDTETAKVWESLGISKDRIHYLSKDHN
FWGPVGSKGPCGPDTEIYVDTGKSKCSLDCNITCSCGKYFEIWNNVFMQYNKDENGNYIELGRKCVDTGMGLERTIAFLQ
GKSSVYDTDAFMPIIKRIEYISGKIYGQKEDDDRCIRIISDHVKAACFILADSSVVFPSNLGQGYVLRRLIRRSIRYAKK
LGIKSHFLADLVDSVEAIYRSFYNELTEKKDFIKKELSKEEEKFFKTLSQGEQEFIKITRNLPSKTIPGDIAFKLYDTYG
FPYEVTEELAIEYGFNVDKLGFNEHFKKHQKTSKKGGDKVFKGGLADYTYETTKLHTATHLLHKALQLVLGDHVRQKGSN
ITAERLRFDFVHSEKMTDDEIKKVEEIVNLQIKNSLSVKKIIMELSEAREKGAMALFGEKYDDLVSVYEIDGFSLEVCGG
PHVENTNELGTFKIQKEQSSSSGIRRIKAILIDE
>Mature_593_residues
TLNKLRKKYIDFFKSKKHFEIMGKSLVPENDPTVLFNTAGMQPLIPYLLGEVHPSGDMLVNVQKCLRTGDIDEVGDLSHL
TFFEMLGNWSLGAYFKEYSVKCSFEFLTSSDYLNIPKDSLYVSVFEGDQEIPRDTETAKVWESLGISKDRIHYLSKDHNF
WGPVGSKGPCGPDTEIYVDTGKSKCSLDCNITCSCGKYFEIWNNVFMQYNKDENGNYIELGRKCVDTGMGLERTIAFLQG
KSSVYDTDAFMPIIKRIEYISGKIYGQKEDDDRCIRIISDHVKAACFILADSSVVFPSNLGQGYVLRRLIRRSIRYAKKL
GIKSHFLADLVDSVEAIYRSFYNELTEKKDFIKKELSKEEEKFFKTLSQGEQEFIKITRNLPSKTIPGDIAFKLYDTYGF
PYEVTEELAIEYGFNVDKLGFNEHFKKHQKTSKKGGDKVFKGGLADYTYETTKLHTATHLLHKALQLVLGDHVRQKGSNI
TAERLRFDFVHSEKMTDDEIKKVEEIVNLQIKNSLSVKKIIMELSEAREKGAMALFGEKYDDLVSVYEIDGFSLEVCGGP
HVENTNELGTFKIQKEQSSSSGIRRIKAILIDE

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=464, Percent_Identity=40.301724137931, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI38569417, Length=461, Percent_Identity=34.2733188720174, Blast_Score=264, Evalue=2e-70,
Organism=Escherichia coli, GI1789048, Length=445, Percent_Identity=36.8539325842697, Blast_Score=294, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17506981, Length=463, Percent_Identity=40.6047516198704, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17536681, Length=446, Percent_Identity=37.4439461883408, Blast_Score=280, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324911, Length=468, Percent_Identity=39.5299145299145, Blast_Score=313, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24582809, Length=445, Percent_Identity=39.3258426966292, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI45552267, Length=445, Percent_Identity=39.3258426966292, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24658214, Length=513, Percent_Identity=31.5789473684211, Blast_Score=218, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 67774; Mature: 67643

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNKLRKKYIDFFKSKKHFEIMGKSLVPENDPTVLFNTAGMQPLIPYLLGEVHPSGDML
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCE
VNVQKCLRTGDIDEVGDLSHLTFFEMLGNWSLGAYFKEYSVKCSFEFLTSSDYLNIPKDS
EEHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCEEEEEEEEECCCCCCCCCCCC
LYVSVFEGDQEIPRDTETAKVWESLGISKDRIHYLSKDHNFWGPVGSKGPCGPDTEIYVD
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
TGKSKCSLDCNITCSCGKYFEIWNNVFMQYNKDENGNYIELGRKCVDTGMGLERTIAFLQ
CCCCEEEEECEEEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GKSSVYDTDAFMPIIKRIEYISGKIYGQKEDDDRCIRIISDHVKAACFILADSSVVFPSN
CCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHEEEEEEECCCEECCCC
LGQGYVLRRLIRRSIRYAKKLGIKSHFLADLVDSVEAIYRSFYNELTEKKDFIKKELSKE
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKFFKTLSQGEQEFIKITRNLPSKTIPGDIAFKLYDTYGFPYEVTEELAIEYGFNVDKL
HHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
GFNEHFKKHQKTSKKGGDKVFKGGLADYTYETTKLHTATHLLHKALQLVLGDHVRQKGSN
CCHHHHHHHHHHHHCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITAERLRFDFVHSEKMTDDEIKKVEEIVNLQIKNSLSVKKIIMELSEAREKGAMALFGEK
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCC
YDDLVSVYEIDGFSLEVCGGPHVENTNELGTFKIQKEQSSSSGIRRIKAILIDE
HHHHHEEEEECCCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLNKLRKKYIDFFKSKKHFEIMGKSLVPENDPTVLFNTAGMQPLIPYLLGEVHPSGDML
CHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCE
VNVQKCLRTGDIDEVGDLSHLTFFEMLGNWSLGAYFKEYSVKCSFEFLTSSDYLNIPKDS
EEHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCEEEEEEEEECCCCCCCCCCCC
LYVSVFEGDQEIPRDTETAKVWESLGISKDRIHYLSKDHNFWGPVGSKGPCGPDTEIYVD
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
TGKSKCSLDCNITCSCGKYFEIWNNVFMQYNKDENGNYIELGRKCVDTGMGLERTIAFLQ
CCCCEEEEECEEEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GKSSVYDTDAFMPIIKRIEYISGKIYGQKEDDDRCIRIISDHVKAACFILADSSVVFPSN
CCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHEEEEEEECCCEECCCC
LGQGYVLRRLIRRSIRYAKKLGIKSHFLADLVDSVEAIYRSFYNELTEKKDFIKKELSKE
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEKFFKTLSQGEQEFIKITRNLPSKTIPGDIAFKLYDTYGFPYEVTEELAIEYGFNVDKL
HHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
GFNEHFKKHQKTSKKGGDKVFKGGLADYTYETTKLHTATHLLHKALQLVLGDHVRQKGSN
CCHHHHHHHHHHHHCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITAERLRFDFVHSEKMTDDEIKKVEEIVNLQIKNSLSVKKIIMELSEAREKGAMALFGEK
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCC
YDDLVSVYEIDGFSLEVCGGPHVENTNELGTFKIQKEQSSSSGIRRIKAILIDE
HHHHHEEEEECCCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA