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The map label for this gene is ldh [H]

Identifier: 15594433

GI number: 15594433

Start: 82970

End: 83920

Strand: Reverse

Name: ldh [H]

Synonym: BB0087

Alternate gene names: 15594433

Gene position: 83920-82970 (Counterclockwise)

Preceding gene: 15594434

Following gene: 15594429

Centisome position: 9.21

GC content: 31.79

Gene sequence:

>951_bases
ATGCTTAAGTCTAATAAAGTTGTTCTTATTGGAGCTGGTGGGGTTGGTTCAAGCTTTGCGTATGCTTTAACAATAGACAA
TTCACTTGTACATGAACTTGTAATTATTGATGTTAATGAAAATAAAGCAAAAGGGGAGGTCATGGACCTTAATCATGGCC
AAATGTTTTTAAAGAAGAATATTAATGTATTGTTTGGGACTTACAAAGATTGTGCTAATGCAGATATTGTTGTAATTACA
GCAGGACTTAATCAAAAGCCTGGTGAGACAAGACTTGATTTGGTTGATAAAAATTCTAAAATTTTTAAAGATATTATAAC
TAATGTTGTATCTAGCGGTTTTGATGGTATTTTTGTTGTTGCAAGCAATCCTGTAGACATTATGACTTATGTTACAATGA
AATATTCCAAATTTCCTATTCATAAGGTTATTGGTACTGGGACTATTCTTGATACTTCAAGACTTAGATATTTTTTAAGT
GATCATTTTAATGTGAACACTCAAAATATACATTCATATATTATGGGTGAGCACRGTGACAGTTCTTTTGCTACGTGGGA
TGAAACAAAAATAGCAATGAAGCCTTTGTCAGAATATCTTGCTGAAGGCAAAATAACTGAGTTGGAGCTTGATGAAATTC
ATAAAAAGGTTGTGAATGCTGCTTATGAAGTTATTAAGTTAAAGGGGGCAACCTATTATGCTATTGGACTTGGTATTAAG
AATATTGTAAATGCAATAATTGGAGATCAGAATGTTATTCTGCCAATATCTTCTTATATTAATGGCCAGTATGGGGGATT
GATTAAAGATATTTATATTGGAGCGCCTGCTATAGTTTGTAAGGAAGGAGTCAAAGAAGTTTTAAACTTTAAGATAAGCC
CTAAAGAGCTTGATAAGTTTAATAGTTCTGCTAATCAGCTTAAAAGCTATATTGATAAAATGGAATTTTAG

Upstream 100 bases:

>100_bases
GGTATGTGATTGGCAAAGCCCTTAGAAATGCGATAGAATATTTTTATCATTACTTATTTAAAATTAGTTTTGTATATAGT
TTTAATTTAATGGAGTAATT

Downstream 100 bases:

>100_bases
TATTAAGACCCCAAATATTTTGGGGGTTTTCATTTTTCAAGCATATTTTCTCCGTTTTTTCTGTGTTCAATTGCTTCAAG
CAATGCTTCTCTTGAAATAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLKSNKVVLIGAGGVGSSFAYALTIDNSLVHELVIIDVNENKAKGEVMDLNHGQMFLKKNINVLFGTYKDCANADIVVIT
AGLNQKPGETRLDLVDKNSKIFKDIITNVVSSGFDGIFVVASNPVDIMTYVTMKYSKFPIHKVIGTGTILDTSRLRYFLS
DHFNVNTQNIHSYIMGEHXDSSFATWDETKIAMKPLSEYLAEGKITELELDEIHKKVVNAAYEVIKLKGATYYAIGLGIK
NIVNAIIGDQNVILPISSYINGQYGGLIKDIYIGAPAIVCKEGVKEVLNFKISPKELDKFNSSANQLKSYIDKMEF

Sequences:

>Translated_316_residues
MLKSNKVVLIGAGGVGSSFAYALTIDNSLVHELVIIDVNENKAKGEVMDLNHGQMFLKKNINVLFGTYKDCANADIVVIT
AGLNQKPGETRLDLVDKNSKIFKDIITNVVSSGFDGIFVVASNPVDIMTYVTMKYSKFPIHKVIGTGTILDTSRLRYFLS
DHFNVNTQNIHSYIMGEHXDSSFATWDETKIAMKPLSEYLAEGKITELELDEIHKKVVNAAYEVIKLKGATYYAIGLGIK
NIVNAIIGDQNVILPISSYINGQYGGLIKDIYIGAPAIVCKEGVKEVLNFKISPKELDKFNSSANQLKSYIDKMEF
>Mature_316_residues
MLKSNKVVLIGAGGVGSSFAYALTIDNSLVHELVIIDVNENKAKGEVMDLNHGQMFLKKNINVLFGTYKDCANADIVVIT
AGLNQKPGETRLDLVDKNSKIFKDIITNVVSSGFDGIFVVASNPVDIMTYVTMKYSKFPIHKVIGTGTILDTSRLRYFLS
DHFNVNTQNIHSYIMGEHXDSSFATWDETKIAMKPLSEYLAEGKITELELDEIHKKVVNAAYEVIKLKGATYYAIGLGIK
NIVNAIIGDQNVILPISSYINGQYGGLIKDIYIGAPAIVCKEGVKEVLNFKISPKELDKFNSSANQLKSYIDKMEF

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=40.9836065573771, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=40.9836065573771, Blast_Score=234, Evalue=5e-62,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=38.2911392405063, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=38.2911392405063, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=36.8253968253968, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI47059044, Length=306, Percent_Identity=37.5816993464052, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI221136809, Length=306, Percent_Identity=37.5816993464052, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=37.0491803278689, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=37.0491803278689, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI260099727, Length=222, Percent_Identity=40.990990990991, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI260099725, Length=189, Percent_Identity=42.8571428571429, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI207028494, Length=200, Percent_Identity=35, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI103472011, Length=318, Percent_Identity=26.1006289308176, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=30.2702702702703, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789632, Length=295, Percent_Identity=26.1016949152542, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=38.5665529010239, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17554310, Length=331, Percent_Identity=26.5861027190332, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136226, Length=315, Percent_Identity=38.0952380952381, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI45550422, Length=309, Percent_Identity=30.7443365695793, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24647881, Length=324, Percent_Identity=25, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24663599, Length=299, Percent_Identity=24.4147157190635, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34846; Mature: 34846

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSNKVVLIGAGGVGSSFAYALTIDNSLVHELVIIDVNENKAKGEVMDLNHGQMFLKKN
CCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEECCCCEEEEECC
INVLFGTYKDCANADIVVITAGLNQKPGETRLDLVDKNSKIFKDIITNVVSSGFDGIFVV
CEEEEECCHHCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIMTYVTMKYSKFPIHKVIGTGTILDTSRLRYFLSDHFNVNTQNIHSYIMGEHXD
ECCCCEEEEEEEEECCCCCHHHHHCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
SSFATWDETKIAMKPLSEYLAEGKITELELDEIHKKVVNAAYEVIKLKGATYYAIGLGIK
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHEEECCCEEEEEECCHH
NIVNAIIGDQNVILPISSYINGQYGGLIKDIYIGAPAIVCKEGVKEVLNFKISPKELDKF
HHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
NSSANQLKSYIDKMEF
CCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKSNKVVLIGAGGVGSSFAYALTIDNSLVHELVIIDVNENKAKGEVMDLNHGQMFLKKN
CCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEECCCCEEEEECC
INVLFGTYKDCANADIVVITAGLNQKPGETRLDLVDKNSKIFKDIITNVVSSGFDGIFVV
CEEEEECCHHCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIMTYVTMKYSKFPIHKVIGTGTILDTSRLRYFLSDHFNVNTQNIHSYIMGEHXD
ECCCCEEEEEEEEECCCCCHHHHHCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
SSFATWDETKIAMKPLSEYLAEGKITELELDEIHKKVVNAAYEVIKLKGATYYAIGLGIK
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHEEECCCEEEEEECCHH
NIVNAIIGDQNVILPISSYINGQYGGLIKDIYIGAPAIVCKEGVKEVLNFKISPKELDKF
HHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
NSSANQLKSYIDKMEF
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA