BacMap

Definition	Borrelia burgdorferi B31 chromosome, complete genome.
Accession	NC_001318
Length	910,724

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The map label for this gene is lepA

Identifier: 15594434

GI number: 15594434

Start: 84041

End: 85720

Strand: Reverse

Name: lepA

Synonym: BB0088

Alternate gene names: 15594434

Gene position: 85720-84041 (Counterclockwise)

Preceding gene: 15594435

Following gene: 15594433

Centisome position: 9.41

GC content: 32.14

Gene sequence:

>1680_bases
ATGCTTGACAGTATGGATATTGAGCGAGAAAGAGGAATTACAATTAAAAGTCAGGCAGTAACAATTACTTATAAAAGTAA
TGATGGTGATTTTTATGAGTTAAATTTTGTAGATACTCCAGGCCATGTTGATTTTTCTTATGAAGTCTCAAGGGCAATTT
CATCTTGTGAGGGTGCTCTTTTATTAATTGATGCAAGTCAAGGAATACAAGCTCAAACAGTTTCTAATTTTTATATGGCT
TTTGAGCATGATTTAGAAATTATTCCCGTTATTAATAAGATAGATCTTCCAAATGCTAATGTTGATTTTGTAAAAAAGCA
AATAAAAAATGATTTAGGATTAAATGAAGAAATTGCTATTTCGATTTCTGCTAAGAATGGAATAGGAATTGATGATTTGC
TTGAAGCTATTTGTAAGTATGTGCCATCTCCTAGGGGAAGTATTAAGGATCCATTAAGAGCATTAATTTTTGATTCTCAT
TATGATTCTTATAGAGGAGTTGTTGTTCACTTTAGGATTTTCGAAGGACAAATCAAAATGGGCGATAAGATTAGGTTAAT
GCATACTAATAGCGAGCATTTAATTGAAGAAATCGGAATTTTTAAAATATCACTTGAAAGAAAAGATACCTTAGAAGCAG
GAGATGTTGGATATTTTATTGCAGGAATAAAAAATATATCAGATGTGAAAATTGGAGATACAGTAACACTTTGTGATTTT
CCCGCATTATCTCCTCTTGAGGGATTTAAAGAAGTTAAGCCTGTAGTATTCTCTTCAGTTTATCCTGTTGATGCTAATCA
ATATGATGATCTTTTAAAGGCAATGGATAGATTAAAACTTAATGATGCATCGTTAACATTTGAAAAAGATTCATCATCTG
CTCTTGGGCATGGTTTTAAGTGTGGATTTCTTGGTCTTTTGCATTTAGAAGTTATTCAAGAACGCATTGAGCGTGAATTT
AATTTAAACGTGATATTGACTTCTCCTTCTGTTAGATACAAAATAATTCCTAAAAAGGGAGAATCTTATTTTATTGAAAC
TCCTGAGCAATTTCCCGGAAATGAAGCTATTGAAAGCGTTCTTGAACCTTATATTAAGGCTAATATTATTGTTCCTACAG
AATTTTTGGGCAATATTATGAGTGTGTGTTTATTGAAAAGAGGAGTTCAGACGAACTTAATTTATCTTGATACTAAGCGT
GTTGAACTTATTTATAAAATGCCTCTTTCTGAAATCCTTTTTGATTTTTATGATAAAATTAAATCTGTGAGCCGTGGATA
TGCTTCTTTTGATTATGAGCTTTTAGATTATGAATATACAGATTTGGTAAGATTAGATATATTAGTTAATGGGGATAGAG
TTGATGCACTCTCCCAGTTGGTTTTTAAAGATAGCGCCAGAACTAAGGCTATTGGGATTTGTAAAAAGTTGAAAGATGAA
ATAGCAAGGCAACAATTTAGAATAGCCATTCAAGGTGCTATTGGCTCTAATGTTATTGCTCGAGAGACAATCTCTCCTGT
TAGAAAAGATGTTACTGCCAAGTGTTATGGTGGTGATATTACTCGTAAGAGAAAACTTTTGGAAAAGCAGAAAGAAGGTA
AAAAGCGAATGAAGATGGTGGGAAATGTTGAGATTCCACAAAGCGCTTTTCTTGCGGTTCTAAAATCCAATGATAATTAA

Upstream 100 bases:

>100_bases
CTTTTGCATTATTGCACACATTGATCATGGTAAATCTACATTAGCTGATCGATTTATTCAAAAAGCTAAAATAATATCTG
ATCGTGATTTTAAAAGTCAA

Downstream 100 bases:

>100_bases
ATTTGTTTTTAATTTTTAAAGGTATGTGATTGGCAAAGCCCTTAGAAATGCGATAGAATATTTTTATCATTACTTATTTA
AAATTAGTTTTGTATATAGT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLDSMDIERERGITIKSQAVTITYKSNDGDFYELNFVDTPGHVDFSYEVSRAISSCEGALLLIDASQGIQAQTVSNFYMA
FEHDLEIIPVINKIDLPNANVDFVKKQIKNDLGLNEEIAISISAKNGIGIDDLLEAICKYVPSPRGSIKDPLRALIFDSH
YDSYRGVVVHFRIFEGQIKMGDKIRLMHTNSEHLIEEIGIFKISLERKDTLEAGDVGYFIAGIKNISDVKIGDTVTLCDF
PALSPLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDSSSALGHGFKCGFLGLLHLEVIQERIEREF
NLNVILTSPSVRYKIIPKKGESYFIETPEQFPGNEAIESVLEPYIKANIIVPTEFLGNIMSVCLLKRGVQTNLIYLDTKR
VELIYKMPLSEILFDFYDKIKSVSRGYASFDYELLDYEYTDLVRLDILVNGDRVDALSQLVFKDSARTKAIGICKKLKDE
IARQQFRIAIQGAIGSNVIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKMVGNVEIPQSAFLAVLKSNDN

Sequences:

>Translated_559_residues
MLDSMDIERERGITIKSQAVTITYKSNDGDFYELNFVDTPGHVDFSYEVSRAISSCEGALLLIDASQGIQAQTVSNFYMA
FEHDLEIIPVINKIDLPNANVDFVKKQIKNDLGLNEEIAISISAKNGIGIDDLLEAICKYVPSPRGSIKDPLRALIFDSH
YDSYRGVVVHFRIFEGQIKMGDKIRLMHTNSEHLIEEIGIFKISLERKDTLEAGDVGYFIAGIKNISDVKIGDTVTLCDF
PALSPLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDSSSALGHGFKCGFLGLLHLEVIQERIEREF
NLNVILTSPSVRYKIIPKKGESYFIETPEQFPGNEAIESVLEPYIKANIIVPTEFLGNIMSVCLLKRGVQTNLIYLDTKR
VELIYKMPLSEILFDFYDKIKSVSRGYASFDYELLDYEYTDLVRLDILVNGDRVDALSQLVFKDSARTKAIGICKKLKDE
IARQQFRIAIQGAIGSNVIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKMVGNVEIPQSAFLAVLKSNDN
>Mature_559_residues
MLDSMDIERERGITIKSQAVTITYKSNDGDFYELNFVDTPGHVDFSYEVSRAISSCEGALLLIDASQGIQAQTVSNFYMA
FEHDLEIIPVINKIDLPNANVDFVKKQIKNDLGLNEEIAISISAKNGIGIDDLLEAICKYVPSPRGSIKDPLRALIFDSH
YDSYRGVVVHFRIFEGQIKMGDKIRLMHTNSEHLIEEIGIFKISLERKDTLEAGDVGYFIAGIKNISDVKIGDTVTLCDF
PALSPLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDSSSALGHGFKCGFLGLLHLEVIQERIEREF
NLNVILTSPSVRYKIIPKKGESYFIETPEQFPGNEAIESVLEPYIKANIIVPTEFLGNIMSVCLLKRGVQTNLIYLDTKR
VELIYKMPLSEILFDFYDKIKSVSRGYASFDYELLDYEYTDLVRLDILVNGDRVDALSQLVFKDSARTKAIGICKKLKDE
IARQQFRIAIQGAIGSNVIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKMVGNVEIPQSAFLAVLKSNDN

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=567, Percent_Identity=48.6772486772487, Blast_Score=576, Evalue=1e-164,
Organism=Homo sapiens, GI25306287, Length=108, Percent_Identity=41.6666666666667, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI19923640, Length=108, Percent_Identity=41.6666666666667, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI25306283, Length=108, Percent_Identity=41.6666666666667, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI18390331, Length=151, Percent_Identity=34.4370860927152, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI94966754, Length=94, Percent_Identity=41.4893617021277, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI217272894, Length=93, Percent_Identity=39.7849462365591, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI217272892, Length=93, Percent_Identity=39.7849462365591, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI4503483, Length=104, Percent_Identity=35.5769230769231, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310132016, Length=71, Percent_Identity=40.8450704225352, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI310110807, Length=71, Percent_Identity=40.8450704225352, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI310123363, Length=71, Percent_Identity=40.8450704225352, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=554, Percent_Identity=49.8194945848375, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI48994988, Length=467, Percent_Identity=29.3361884368308, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789738, Length=116, Percent_Identity=37.9310344827586, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789559, Length=156, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=575, Percent_Identity=41.0434782608696, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=421, Percent_Identity=23.9904988123515, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=141, Percent_Identity=34.7517730496454, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17552882, Length=137, Percent_Identity=33.5766423357664, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=94, Percent_Identity=40.4255319148936, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=94, Percent_Identity=40.4255319148936, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=563, Percent_Identity=43.8721136767318, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6323098, Length=149, Percent_Identity=33.5570469798658, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=104, Percent_Identity=36.5384615384615, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=104, Percent_Identity=36.5384615384615, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=242, Percent_Identity=25.6198347107438, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=73, Percent_Identity=38.3561643835616, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78706572, Length=563, Percent_Identity=40.8525754884547, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=33.7837837837838, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221458488, Length=128, Percent_Identity=35.15625, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28574573, Length=98, Percent_Identity=38.7755102040816, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572034, Length=178, Percent_Identity=29.7752808988764, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21357743, Length=93, Percent_Identity=38.7096774193548, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_BORBU (O51115)

Other databases:

- EMBL:   AE000783
- PIR:   H70110
- RefSeq:   NP_212222.1
- ProteinModelPortal:   O51115
- SMR:   O51115
- EnsemblBacteria:   EBBORT00000008448
- GeneID:   1194924
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0088
- NMPDR:   fig|224326.1.peg.472
- TIGR:   BB_0088
- GeneTree:   EBGT00050000007017
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   BBUR224326:BB_0088-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 62901; Mature: 62901

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSMDIERERGITIKSQAVTITYKSNDGDFYELNFVDTPGHVDFSYEVSRAISSCEGAL
CCCCCCCHHHCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEE
LLIDASQGIQAQTVSNFYMAFEHDLEIIPVINKIDLPNANVDFVKKQIKNDLGLNEEIAI
EEEECCCCCCHHHHHHHHEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
SISAKNGIGIDDLLEAICKYVPSPRGSIKDPLRALIFDSHYDSYRGVVVHFRIFEGQIKM
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECEEEC
GDKIRLMHTNSEHLIEEIGIFKISLERKDTLEAGDVGYFIAGIKNISDVKIGDTVTLCDF
CCEEEEEECCHHHHHHHCCEEEEEECCCCCCCCCCCEEEEECCCCCCCEECCCEEEEECC
PALSPLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDSSSALGHGFK
CCCCHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
CGFLGLLHLEVIQERIEREFNLNVILTSPSVRYKIIPKKGESYFIETPEQFPGNEAIESV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCEEEEECCCCCCCHHHHHHH
LEPYIKANIIVPTEFLGNIMSVCLLKRGVQTNLIYLDTKRVELIYKMPLSEILFDFYDKI
HHHHHHEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCHHHHHHHHHHHH
KSVSRGYASFDYELLDYEYTDLVRLDILVNGDRVDALSQLVFKDSARTKAIGICKKLKDE
HHHHCCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IARQQFRIAIQGAIGSNVIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKMV
HHHHHEEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GNVEIPQSAFLAVLKSNDN
CCCCCCHHHEEEEEECCCC
>Mature Secondary Structure
MLDSMDIERERGITIKSQAVTITYKSNDGDFYELNFVDTPGHVDFSYEVSRAISSCEGAL
CCCCCCCHHHCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEE
LLIDASQGIQAQTVSNFYMAFEHDLEIIPVINKIDLPNANVDFVKKQIKNDLGLNEEIAI
EEEECCCCCCHHHHHHHHEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
SISAKNGIGIDDLLEAICKYVPSPRGSIKDPLRALIFDSHYDSYRGVVVHFRIFEGQIKM
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECEEEC
GDKIRLMHTNSEHLIEEIGIFKISLERKDTLEAGDVGYFIAGIKNISDVKIGDTVTLCDF
CCEEEEEECCHHHHHHHCCEEEEEECCCCCCCCCCCEEEEECCCCCCCEECCCEEEEECC
PALSPLEGFKEVKPVVFSSVYPVDANQYDDLLKAMDRLKLNDASLTFEKDSSSALGHGFK
CCCCHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
CGFLGLLHLEVIQERIEREFNLNVILTSPSVRYKIIPKKGESYFIETPEQFPGNEAIESV
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCEEEEECCCCCCCHHHHHHH
LEPYIKANIIVPTEFLGNIMSVCLLKRGVQTNLIYLDTKRVELIYKMPLSEILFDFYDKI
HHHHHHEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCHHHHHHHHHHHH
KSVSRGYASFDYELLDYEYTDLVRLDILVNGDRVDALSQLVFKDSARTKAIGICKKLKDE
HHHHCCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IARQQFRIAIQGAIGSNVIARETISPVRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKMV
HHHHHEEEEEEECCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GNVEIPQSAFLAVLKSNDN
CCCCCCHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685