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The map label for this gene is def

Identifier: 15594411

GI number: 15594411

Start: 60036

End: 60554

Strand: Reverse

Name: def

Synonym: BB0065

Alternate gene names: 15594411

Gene position: 60554-60036 (Counterclockwise)

Preceding gene: 15594412

Following gene: 15594410

Centisome position: 6.65

GC content: 33.33

Gene sequence:

>519_bases
TTGAAAGGGGGATGGGTTTTTATGGAAATGGTATTTTATCCTAATGATTTGCTTCGTGTTAAGACAAAGCAAATCGAGAA
TATTGACGATAAGATTAGAGATTATGCTAAAAAGATGATAGAATTGATGGATATTAGCGGTGGAGTTGGGCTTGCTGCTC
CCCAAGTAGGCCTTGATTTGGCGCTTTTTGTAGTTAGAGAGAATAAAATGGCAAGGCCTTTGGTTTTTATCAATCCTTCA
ATCATAGAGACTTCTTATGAGTTTAGTTCTTACAAAGAGGGGTGTTTGAGTATACCAGGAGTTTATTATGATTTAATGAG
GCCTAAAGCTGTTGTAATAAATTTTCATGATGAGAATGGAAAATCTTTTACTATAGAAAATTCTGATTTTTTGGCAAGAA
TTATTCAGCACGAAATGGACCATTTAAATGGAGTTCTTTTTATTGATTATTACGAAGAAAAGTTAAAAAATAAATTACTA
AAGCCTTATATGAGAGAAAGGGGGCTTAAGGCAAAATGA

Upstream 100 bases:

>100_bases
TATGCTATTCCTAAGTCTAGGATTGGTAATATTTATTTTTCATTATTGGGAAATGAAGAATGGAAGCTTTTTGGAAAAAT
AAAATAAGTGTCTTTTTTGT

Downstream 100 bases:

>100_bases
AAATATTTTTTGTAAGCTCTTCTTCTATTGCTTTAGAAGTTTTCAAAGAGATTGTAAAGCATTATGAAGTGGTGGGAGTT
TTGACTTTGCCTGATAGACC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKGGWVFMEMVFYPNDLLRVKTKQIENIDDKIRDYAKKMIELMDISGGVGLAAPQVGLDLALFVVRENKMARPLVFINPS
IIETSYEFSSYKEGCLSIPGVYYDLMRPKAVVINFHDENGKSFTIENSDFLARIIQHEMDHLNGVLFIDYYEEKLKNKLL
KPYMRERGLKAK

Sequences:

>Translated_172_residues
MKGGWVFMEMVFYPNDLLRVKTKQIENIDDKIRDYAKKMIELMDISGGVGLAAPQVGLDLALFVVRENKMARPLVFINPS
IIETSYEFSSYKEGCLSIPGVYYDLMRPKAVVINFHDENGKSFTIENSDFLARIIQHEMDHLNGVLFIDYYEEKLKNKLL
KPYMRERGLKAK
>Mature_172_residues
MKGGWVFMEMVFYPNDLLRVKTKQIENIDDKIRDYAKKMIELMDISGGVGLAAPQVGLDLALFVVRENKMARPLVFINPS
IIETSYEFSSYKEGCLSIPGVYYDLMRPKAVVINFHDENGKSFTIENSDFLARIIQHEMDHLNGVLFIDYYEEKLKNKLL
KPYMRERGLKAK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=141, Percent_Identity=34.0425531914894, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=37.888198757764, Blast_Score=96, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=29.9270072992701, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=31.3253012048193, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BORBU (O51092)

Other databases:

- EMBL:   AE000783
- PIR:   A70108
- RefSeq:   NP_212199.1
- ProteinModelPortal:   O51092
- SMR:   O51092
- EnsemblBacteria:   EBBORT00000008621
- GeneID:   1194901
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0065
- NMPDR:   fig|224326.1.peg.449
- TIGR:   BB_0065
- GeneTree:   EBGT00050000007440
- HOGENOM:   HBG665227
- OMA:   LMDISGG
- ProtClustDB:   PRK00150
- BioCyc:   BBUR224326:BB_0065-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19923; Mature: 19923

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGGWVFMEMVFYPNDLLRVKTKQIENIDDKIRDYAKKMIELMDISGGVGLAAPQVGLDL
CCCCEEEEEEEECCCHHEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHE
ALFVVRENKMARPLVFINPSIIETSYEFSSYKEGCLSIPGVYYDLMRPKAVVINFHDENG
EEEEEECCCCCCCEEEECCHHEECCCCHHHHCCCCCCCCCHHHHHCCCEEEEEEEECCCC
KSFTIENSDFLARIIQHEMDHLNGVLFIDYYEEKLKNKLLKPYMRERGLKAK
CEEEEECHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKGGWVFMEMVFYPNDLLRVKTKQIENIDDKIRDYAKKMIELMDISGGVGLAAPQVGLDL
CCCCEEEEEEEECCCHHEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHE
ALFVVRENKMARPLVFINPSIIETSYEFSSYKEGCLSIPGVYYDLMRPKAVVINFHDENG
EEEEEECCCCCCCEEEECCHHEECCCCHHHHCCCCCCCCCHHHHHCCCEEEEEEEECCCC
KSFTIENSDFLARIIQHEMDHLNGVLFIDYYEEKLKNKLLKPYMRERGLKAK
CEEEEECHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685