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The map label for this gene is fmt [H]

Identifier: 15594410

GI number: 15594410

Start: 59101

End: 60039

Strand: Reverse

Name: fmt [H]

Synonym: BB0064

Alternate gene names: 15594410

Gene position: 60039-59101 (Counterclockwise)

Preceding gene: 15594411

Following gene: 15594409

Centisome position: 6.59

GC content: 30.99

Gene sequence:

>939_bases
ATGAAAATATTTTTTGTAAGCTCTTCTTCTATTGCTTTAGAAGTTTTCAAAGAGATTGTAAAGCATTATGAAGTGGTGGG
AGTTTTGACTTTGCCTGATAGACCCAAGGGTAGAGGACAAAAGTTAAGTCAAAATGTAATAAAGTCAGAGGCTATTGCTA
GGAATATTAAAGTTCTTGATCCTTTGATTCTTGATGATAATGTATTAAATTTAGTTAGAGATTTGAATCCAGATTTAATG
TTAGTTTTTTCTTATGGCAAGATTTTTAAAAAAGAATTTTTAGATCTTTTCCCAAAGGGATGTATTAATGTTCATCCTTC
TCTTTTACCCAAATATAGAGGCGTTTCTCCGATTCAATCTGCAATTTTAAATGGTGATTGTGTTAGCGGTGTTACTATTC
AGAGTATGGCTTTAGAAATGGATAGTGGCAATATTTTAGTGCAAAAAAATTTTAAAATAAGATCTTATGATACAAGTCAT
GATATTTCAAAACTTGTATCAAGCCTTAGTCCAAGCCTTGTTCTAGAAGCTTTGGAAAAAATTAGCAAGGGTTTTTTGGG
AATTCCTCAAAAATCAAGCGAAGCTACTTTTTGTTCTTTTTTAAAAAAAGAATCTGGATTTATAGATTTTAATTTAAGTG
CATTTGAGATTAAAAATAAAATTAATGCGTGCAATCCTTGGCCACTTGTAAGAGTTAGGCTTGATTATAACGACATTATT
TTTCATCGCGCCGATTTTTTGGAGGTAGATTTATATAAGGAGAGAAAAATAGGAGAAATTGTTGATTTTAATCCTGAAAA
AGGATTATTTGTTAATACCGGGAAAGGGATTCTTTTGCTTTTAGAAGTTCAAAGACCTGGAAGAAAGGTTTTGGATTTTA
AGTCCTTTTATAATGGAAGTAGGCAGCTAATAGGGCAAGTGTTTTCTTCAATAGAATAG

Upstream 100 bases:

>100_bases
TGGACCATTTAAATGGAGTTCTTTTTATTGATTATTACGAAGAAAAGTTAAAAAATAAATTACTAAAGCCTTATATGAGA
GAAAGGGGGCTTAAGGCAAA

Downstream 100 bases:

>100_bases
GGGGGGATATATTAGTTTTAATAATAATGACAATCTAGAAAATAAATTTTCTAATGAAGGGGAATTGTTTAAAAATAAAT
TAGATCTGTCTCAGAGTGAC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKIFFVSSSSIALEVFKEIVKHYEVVGVLTLPDRPKGRGQKLSQNVIKSEAIARNIKVLDPLILDDNVLNLVRDLNPDLM
LVFSYGKIFKKEFLDLFPKGCINVHPSLLPKYRGVSPIQSAILNGDCVSGVTIQSMALEMDSGNILVQKNFKIRSYDTSH
DISKLVSSLSPSLVLEALEKISKGFLGIPQKSSEATFCSFLKKESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDII
FHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLDFKSFYNGSRQLIGQVFSSIE

Sequences:

>Translated_312_residues
MKIFFVSSSSIALEVFKEIVKHYEVVGVLTLPDRPKGRGQKLSQNVIKSEAIARNIKVLDPLILDDNVLNLVRDLNPDLM
LVFSYGKIFKKEFLDLFPKGCINVHPSLLPKYRGVSPIQSAILNGDCVSGVTIQSMALEMDSGNILVQKNFKIRSYDTSH
DISKLVSSLSPSLVLEALEKISKGFLGIPQKSSEATFCSFLKKESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDII
FHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLDFKSFYNGSRQLIGQVFSSIE
>Mature_312_residues
MKIFFVSSSSIALEVFKEIVKHYEVVGVLTLPDRPKGRGQKLSQNVIKSEAIARNIKVLDPLILDDNVLNLVRDLNPDLM
LVFSYGKIFKKEFLDLFPKGCINVHPSLLPKYRGVSPIQSAILNGDCVSGVTIQSMALEMDSGNILVQKNFKIRSYDTSH
DISKLVSSLSPSLVLEALEKISKGFLGIPQKSSEATFCSFLKKESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDII
FHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLDFKSFYNGSRQLIGQVFSSIE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=141, Percent_Identity=29.0780141843972, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI238814322, Length=253, Percent_Identity=24.5059288537549, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=33.3333333333333, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788589, Length=213, Percent_Identity=30.5164319248826, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI133930964, Length=327, Percent_Identity=23.2415902140673, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571984, Length=230, Percent_Identity=29.1304347826087, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550868, Length=230, Percent_Identity=29.1304347826087, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 35107; Mature: 35107

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFFVSSSSIALEVFKEIVKHYEVVGVLTLPDRPKGRGQKLSQNVIKSEAIARNIKVLD
CEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHC
PLILDDNVLNLVRDLNPDLMLVFSYGKIFKKEFLDLFPKGCINVHPSLLPKYRGVSPIQS
CEEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHH
AILNGDCVSGVTIQSMALEMDSGNILVQKNFKIRSYDTSHDISKLVSSLSPSLVLEALEK
HHHCCCCCCCCEEEHEEEEECCCCEEEEECCEEEECCCHHHHHHHHHHCCHHHHHHHHHH
ISKGFLGIPQKSSEATFCSFLKKESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDII
HHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCEE
FHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLDFKSFYNGS
EECCCEEEEEEHHCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCHH
RQLIGQVFSSIE
HHHHHHHHHHCC
>Mature Secondary Structure
MKIFFVSSSSIALEVFKEIVKHYEVVGVLTLPDRPKGRGQKLSQNVIKSEAIARNIKVLD
CEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHC
PLILDDNVLNLVRDLNPDLMLVFSYGKIFKKEFLDLFPKGCINVHPSLLPKYRGVSPIQS
CEEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHH
AILNGDCVSGVTIQSMALEMDSGNILVQKNFKIRSYDTSHDISKLVSSLSPSLVLEALEK
HHHCCCCCCCCEEEHEEEEECCCCEEEEECCEEEECCCHHHHHHHHHHCCHHHHHHHHHH
ISKGFLGIPQKSSEATFCSFLKKESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDII
HHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCEE
FHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLDFKSFYNGS
EECCCEEEEEEHHCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCHH
RQLIGQVFSSIE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA