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The map label for this gene is rsmE1

Identifier: 15594408

GI number: 15594408

Start: 57259

End: 57975

Strand: Direct

Name: rsmE1

Synonym: BB0062

Alternate gene names: 15594408

Gene position: 57259-57975 (Clockwise)

Preceding gene: 15594399

Following gene: 15594414

Centisome position: 6.29

GC content: 25.24

Gene sequence:

>717_bases
ATGAATCTAATGTTAATAACTTTTAATGAATTTAAAACAGGAATTTCACTCAATGATACTAGGGCAGAACATCTTGTTAA
AATCTTAAAATTAAAAGATAACGACAAATTTAAATTTGGTATTCTTGGAGAAAAAAACATTTACCACTGCATTTACAAAA
AAGATAAAAAACTATTTTTCAAGAAAATCTTTAAAGTAGGTGAATCTAATAAACTTAAAAAATTATATGTGCTAATTGGA
ATGATAAGACCAATTGTTGCCAAAAGAATCATAAAAGAACTTGCTAGCATTGGAACATACAAAATAATTTTTTTTAACAC
TGAGCTTACTGAAAAATCATATTTAAATTCTAAAATCTTTAAAAACAATGATTGCGAAAAACATTTAATAGCCGGAGCAA
TGCAAGGAAAAATAACATACTTACCAAAAATAAAAATTATAAAAAATTTGAAAGAAAGCCTTAAATATATTCAACAAGAA
AATTTTGAAATAAAAATATTGCTTGAAAAAAATAGTGAAAAAAATTTAATTGATATAGAACAAATAAACAATGCGGTCAT
TATTGTGGGACCTGAAAGAGGATTTACAGAAAAAGAAAAACAATTAATAGCACAATATAATTTTTCCCCTTACAGCATAT
CCACAAACACCCTGAGAACAGAAACAGCCACAATTGCTGCATCTATTATTACAGCATCAAAACTAATTAATATGTGA

Upstream 100 bases:

>100_bases
ATCTTCTTCGGTTAAAGAAATAGCCATATATTTTACATGCCCCCCTATCGACTTAAAATTATATCACAAATACAATATTA
ATAATAAGGAATTATAAGAA

Downstream 100 bases:

>100_bases
TTTTATATTAAAAATTTTTTTATCATTTTAAATCAATATGTTTTTGAGCACAATTAATTTATAACTGTTTGATTAATCAG
CTTATCATAAATATAAAGCT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase 1

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNLMLITFNEFKTGISLNDTRAEHLVKILKLKDNDKFKFGILGEKNIYHCIYKKDKKLFFKKIFKVGESNKLKKLYVLIG
MIRPIVAKRIIKELASIGTYKIIFFNTELTEKSYLNSKIFKNNDCEKHLIAGAMQGKITYLPKIKIIKNLKESLKYIQQE
NFEIKILLEKNSEKNLIDIEQINNAVIIVGPERGFTEKEKQLIAQYNFSPYSISTNTLRTETATIAASIITASKLINM

Sequences:

>Translated_238_residues
MNLMLITFNEFKTGISLNDTRAEHLVKILKLKDNDKFKFGILGEKNIYHCIYKKDKKLFFKKIFKVGESNKLKKLYVLIG
MIRPIVAKRIIKELASIGTYKIIFFNTELTEKSYLNSKIFKNNDCEKHLIAGAMQGKITYLPKIKIIKNLKESLKYIQQE
NFEIKILLEKNSEKNLIDIEQINNAVIIVGPERGFTEKEKQLIAQYNFSPYSISTNTLRTETATIAASIITASKLINM
>Mature_238_residues
MNLMLITFNEFKTGISLNDTRAEHLVKILKLKDNDKFKFGILGEKNIYHCIYKKDKKLFFKKIFKVGESNKLKKLYVLIG
MIRPIVAKRIIKELASIGTYKIIFFNTELTEKSYLNSKIFKNNDCEKHLIAGAMQGKITYLPKIKIIKNLKESLKYIQQE
NFEIKILLEKNSEKNLIDIEQINNAVIIVGPERGFTEKEKQLIAQYNFSPYSISTNTLRTETATIAASIITASKLINM

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME1_BORBU (O51089)

Other databases:

- EMBL:   AE000783
- PIR:   F70107
- RefSeq:   NP_212196.1
- ProteinModelPortal:   O51089
- EnsemblBacteria:   EBBORT00000008712
- GeneID:   1194898
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0062
- NMPDR:   fig|224326.1.peg.446
- TIGR:   BB_0062
- GeneTree:   EBGT00050000006713
- HOGENOM:   HBG354463
- OMA:   FKFGILG
- ProtClustDB:   PRK11713
- BioCyc:   BBUR224326:BB_0062-MONOMER
- GO:   GO:0005737
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA

EC number: =2.1.1.193

Molecular weight: Translated: 27493; Mature: 27493

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLMLITFNEFKTGISLNDTRAEHLVKILKLKDNDKFKFGILGEKNIYHCIYKKDKKLFF
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCHHHHH
KKIFKVGESNKLKKLYVLIGMIRPIVAKRIIKELASIGTYKIIFFNTELTEKSYLNSKIF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCCCC
KNNDCEKHLIAGAMQGKITYLPKIKIIKNLKESLKYIQQENFEIKILLEKNSEKNLIDIE
CCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEHH
QINNAVIIVGPERGFTEKEKQLIAQYNFSPYSISTNTLRTETATIAASIITASKLINM
HCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLMLITFNEFKTGISLNDTRAEHLVKILKLKDNDKFKFGILGEKNIYHCIYKKDKKLFF
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCHHHHH
KKIFKVGESNKLKKLYVLIGMIRPIVAKRIIKELASIGTYKIIFFNTELTEKSYLNSKIF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCCCC
KNNDCEKHLIAGAMQGKITYLPKIKIIKNLKESLKYIQQENFEIKILLEKNSEKNLIDIE
CCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEHH
QINNAVIIVGPERGFTEKEKQLIAQYNFSPYSISTNTLRTETATIAASIITASKLINM
HCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685