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The map label for this gene is trpS

Identifier: 15594351

GI number: 15594351

Start: 5251

End: 6312

Strand: Reverse

Name: trpS

Synonym: BB0005

Alternate gene names: 15594351

Gene position: 6312-5251 (Counterclockwise)

Preceding gene: 15594352

Following gene: 15594349

Centisome position: 0.69

GC content: 32.49

Gene sequence:

>1062_bases
TTGATTTTGAAAAGAAAGGTTATGCTTACGGGAGATAGGCCTACTGGCGCTCTTCATTTGGGGCACTATGTGGGGTCTGT
AGTAAATCGGTTAAAATTTCAAGAAGAGTATGAAACATATTTTATTATAGCCGATTTACATACTTTGACTACAAAGCCTG
ACTTGAAGAGCATCAATACAATACCTTCTAATGTTAGAGAAATGGTTTTAGATTATCTTGCTTGTGGAATTAATCCTGAT
AAGGTTAGTATATATCTGCAATCAGCCATACCCGAGCTTTTTGAGCTTCATTTAATATTTTCAATGATTGTTACTGTTGC
GCGCTTGCAAAGGATTCCAAGTATAAAAGACATGAGCATTGCAGCTGGACTTAAAGAGATTCCTTATGGCCTTTTGGGGT
ATCCTGTTCTTATGAGTGCAGATATTTTAATGACAAAAGCAAATTTAGTTCCCGTTGGGCGTGATAATGAATCTCATATT
GAATTTGCAAGAGAGCTTGCACGAAGGTTTAACCATTTGTACAAAAATAATTTTTTCCCAATACCCGAATCTGTTTTCAC
AGATTCTCGTCCTTTGGTGGGTATTTATGGCAAAAATAAAATGAGCAAGAGCCTTGATAATGCAATTTTTTTAAACGACG
ATGAAAATTTATTAGAAAAAAAAATTATGTCTATGTATACAGATCCAAATAGGATAAGGGCAGATATTCCTGGCAATGTT
GAAGGTAATCCCATTTTTATTTATCACAGTTTTTTTAATAGTAATCATGAAGAAGTTGAGGATCTTAAAAGTAGGTATAA
GAAGGGTAAAGTAGGTGATGTTGAGGTTAAGAAAAAATTGTTTTTAGCTTTGAATAGTTTTTTAAAGCCAATAAGAGATA
AAAGAAGTTTTTATGAAGCCAAAAAAAAAGATTACATTGATGAAATTATTTTTGATGGTACAAGCAAGGCGAGGTTTATT
GCAAATAAAGTAGTAAAAGATGTTAAGGATTTAATAGGGCTTTCAAAAACTTGGAATGGAATAAAATATAGCGCTGAGAA
AAAATTAAAAAATGAAGAATGA

Upstream 100 bases:

>100_bases
TAGTTGATAATGTGAGTTGGCTTAAGTCTTTTTCTGTATTTTTGGGTGGCTCTGAGATTTTAAGTAATATTAGCCATATT
TCAAGTAAAGATAAGGAGAT

Downstream 100 bases:

>100_bases
AAATTATTGATTTTATGCTAGACTTATATTAGTAATTAACTTATTATTTAAATCAAACAATATTAATTATTAGGTAGATT
TGCCTTTTTTATTAATTTTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILKRKVMLTGDRPTGALHLGHYVGSVVNRLKFQEEYETYFIIADLHTLTTKPDLKSINTIPSNVREMVLDYLACGINPD
KVSIYLQSAIPELFELHLIFSMIVTVARLQRIPSIKDMSIAAGLKEIPYGLLGYPVLMSADILMTKANLVPVGRDNESHI
EFARELARRFNHLYKNNFFPIPESVFTDSRPLVGIYGKNKMSKSLDNAIFLNDDENLLEKKIMSMYTDPNRIRADIPGNV
EGNPIFIYHSFFNSNHEEVEDLKSRYKKGKVGDVEVKKKLFLALNSFLKPIRDKRSFYEAKKKDYIDEIIFDGTSKARFI
ANKVVKDVKDLIGLSKTWNGIKYSAEKKLKNEE

Sequences:

>Translated_353_residues
MILKRKVMLTGDRPTGALHLGHYVGSVVNRLKFQEEYETYFIIADLHTLTTKPDLKSINTIPSNVREMVLDYLACGINPD
KVSIYLQSAIPELFELHLIFSMIVTVARLQRIPSIKDMSIAAGLKEIPYGLLGYPVLMSADILMTKANLVPVGRDNESHI
EFARELARRFNHLYKNNFFPIPESVFTDSRPLVGIYGKNKMSKSLDNAIFLNDDENLLEKKIMSMYTDPNRIRADIPGNV
EGNPIFIYHSFFNSNHEEVEDLKSRYKKGKVGDVEVKKKLFLALNSFLKPIRDKRSFYEAKKKDYIDEIIFDGTSKARFI
ANKVVKDVKDLIGLSKTWNGIKYSAEKKLKNEE
>Mature_353_residues
MILKRKVMLTGDRPTGALHLGHYVGSVVNRLKFQEEYETYFIIADLHTLTTKPDLKSINTIPSNVREMVLDYLACGINPD
KVSIYLQSAIPELFELHLIFSMIVTVARLQRIPSIKDMSIAAGLKEIPYGLLGYPVLMSADILMTKANLVPVGRDNESHI
EFARELARRFNHLYKNNFFPIPESVFTDSRPLVGIYGKNKMSKSLDNAIFLNDDENLLEKKIMSMYTDPNRIRADIPGNV
EGNPIFIYHSFFNSNHEEVEDLKSRYKKGKVGDVEVKKKLFLALNSFLKPIRDKRSFYEAKKKDYIDEIIFDGTSKARFI
ANKVVKDVKDLIGLSKTWNGIKYSAEKKLKNEE

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=346, Percent_Identity=32.3699421965318, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI41352700, Length=188, Percent_Identity=38.8297872340425, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1789786, Length=344, Percent_Identity=29.6511627906977, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71982800, Length=339, Percent_Identity=26.8436578171091, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982793, Length=339, Percent_Identity=26.8436578171091, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320474, Length=356, Percent_Identity=31.4606741573034, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24666151, Length=351, Percent_Identity=33.6182336182336, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_BORBU (O51038)

Other databases:

- EMBL:   AE000783
- PIR:   E70100
- RefSeq:   NP_212139.1
- ProteinModelPortal:   O51038
- SMR:   O51038
- EnsemblBacteria:   EBBORT00000008646
- GeneID:   1194841
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0005
- NMPDR:   fig|224326.1.peg.389
- TIGR:   BB_0005
- GeneTree:   EBGT00050000007460
- HOGENOM:   HBG293263
- OMA:   DNESHIE
- PhylomeDB:   O51038
- ProtClustDB:   PRK00927
- BioCyc:   BBUR224326:BB_0005-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 40419; Mature: 40419

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKRKVMLTGDRPTGALHLGHYVGSVVNRLKFQEEYETYFIIADLHTLTTKPDLKSINT
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCHHHHHC
IPSNVREMVLDYLACGINPDKVSIYLQSAIPELFELHLIFSMIVTVARLQRIPSIKDMSI
CHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AAGLKEIPYGLLGYPVLMSADILMTKANLVPVGRDNESHIEFARELARRFNHLYKNNFFP
HHHHHHCCHHHHCHHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
IPESVFTDSRPLVGIYGKNKMSKSLDNAIFLNDDENLLEKKIMSMYTDPNRIRADIPGNV
CCHHHHCCCCCEEEECCCCHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCC
EGNPIFIYHSFFNSNHEEVEDLKSRYKKGKVGDVEVKKKLFLALNSFLKPIRDKRSFYEA
CCCEEEEEEHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKDYIDEIIFDGTSKARFIANKVVKDVKDLIGLSKTWNGIKYSAEKKLKNEE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCEECHHHHCCCCC
>Mature Secondary Structure
MILKRKVMLTGDRPTGALHLGHYVGSVVNRLKFQEEYETYFIIADLHTLTTKPDLKSINT
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCHHHHHC
IPSNVREMVLDYLACGINPDKVSIYLQSAIPELFELHLIFSMIVTVARLQRIPSIKDMSI
CHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
AAGLKEIPYGLLGYPVLMSADILMTKANLVPVGRDNESHIEFARELARRFNHLYKNNFFP
HHHHHHCCHHHHCHHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
IPESVFTDSRPLVGIYGKNKMSKSLDNAIFLNDDENLLEKKIMSMYTDPNRIRADIPGNV
CCHHHHCCCCCEEEECCCCHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCC
EGNPIFIYHSFFNSNHEEVEDLKSRYKKGKVGDVEVKKKLFLALNSFLKPIRDKRSFYEA
CCCEEEEEEHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKDYIDEIIFDGTSKARFIANKVVKDVKDLIGLSKTWNGIKYSAEKKLKNEE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCEECHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685