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The map label for this gene is alaT

Identifier: 50812284

GI number: 50812284

Start: 3225772

End: 3226932

Strand: Reverse

Name: alaT

Synonym: BSU31400

Alternate gene names: 50812284

Gene position: 3226932-3225772 (Counterclockwise)

Preceding gene: 16080193

Following gene: 16080191

Centisome position: 76.55

GC content: 47.46

Gene sequence:

>1161_bases
ATGACTTCGTATTTATCAGACTATGTACAACAAATAAAACCATCCGGCATCCGCAAATTCTTTGATTTGGCGGCAACGAT
GGAAGGCGTCATTTCTTTAGGCGTCGGCGAGCCTGATTTTGTTACCGCATGGAATGTCCGCGAAGCAAGCATTCTCTCTC
TTGAACAAGGATATACGTCATACACGGCCAATGCGGGTTTATACTCATTGCGGGAGGAAATCAGCCGTTATTTGAGCAAC
AGGTTTGACCTTAGCTACTCGCCAGATAATGAGCTGATTGTGACAGTGGGGGCAAGTCAGGCTTTGGATATTGCGATCCG
CGCCATTGTGAATCCAGGTGAGGAAGTCATCATTCCTGAGCCGTGTTTTGTTGCGTACGATGCGCTTGTTTCTTTGGCAG
GCGGTATCCCGGTTCACGTTCATACAACGGCAGACAAAGGATTTAAAGCAACGGCTGCTGATTTTGAAGCGGCAGTCACT
GAAAAAACAAAGGCCATTCTCATCTGCTCACCATCGAATCCGACAGGTTCGGTTTATTCGAAGGAAGAGCTGAATGAGAT
TGCTGAGTTTGCCAAAAAACATGATGTCATCGTCTTAGCCGACGAGATCTACGCGGAGCTGACATATGATGAGGAATTTA
CAAGTATAGCGGCATTACCGGGAATGAAGGAACGGACGGTAGTCATCTCAGGCTTTTCAAAAGCATTTGCAATGACGGGC
TGGAGGCTCGGTTTTGCCGCGGCACCATCCTTGCTTCGGGATGCCATGCTTAAAATTCATCAGTATGCGATGATGTGCGC
GCCCGCAATGGCGCAGTTTGCCGCCCTTGAAGGCCTGAAGAACGGCATGGAAGACGTAGAAAAAATGAAAAAAAGCTATC
GAAGAAGACGGAATCTGTTTGTAGAATCGCTCAATGAAATCGGTCTCAGCTGTCATCATCCGGGCGGCGCTTTCTATGCT
TTTCCATCTATCAAAAGCATGGGAATGAGTTCAGAACAGTTTGCCGAGGAGCTACTGACGCAGGAAAAAGTGGCAGTTGT
TCCTGGAAGTGTGTTTGGTCCGAGCGGTGAGGGATATATCCGTTGTTCATACGCAACCTCGATTGAACAGCTTCAAGAAG
CGTTAGTCAGAATGAAACGCTTCCTGCACAAAACAACATAA

Upstream 100 bases:

>100_bases
TGTATCTACGACAACCCATTTTATCCTGAAAAAATACAAGCATGACGGCAAAGTGTTTGAAACAGGAGACGACGACAAAA
GAATCGTGGTGTCACCGTAA

Downstream 100 bases:

>100_bases
AAAAAGATACGAACCTTTTGTTCGCATCCAATAAAAGGGGGGAATACTAGAAAGCCTTTGGTATTTTCAGTTTACCCCCT
TTTTGCTGTGCATAAACCTG

Product: hypothetical protein

Products: N-succinyl-2-L-amino-6-oxoheptanedioate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTSYTANAGLYSLREEISRYLSN
RFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPEPCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVT
EKTKAILICSPSNPTGSVYSKEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG
WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLFVESLNEIGLSCHHPGGAFYA
FPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYIRCSYATSIEQLQEALVRMKRFLHKTT

Sequences:

>Translated_386_residues
MTSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTSYTANAGLYSLREEISRYLSN
RFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPEPCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVT
EKTKAILICSPSNPTGSVYSKEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG
WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLFVESLNEIGLSCHHPGGAFYA
FPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYIRCSYATSIEQLQEALVRMKRFLHKTT
>Mature_385_residues
TSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTSYTANAGLYSLREEISRYLSNR
FDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPEPCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVTE
KTKAILICSPSNPTGSVYSKEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTGW
RLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLFVESLNEIGLSCHHPGGAFYAF
PSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYIRCSYATSIEQLQEALVRMKRFLHKTT

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI95147551, Length=394, Percent_Identity=26.1421319796954, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI169881279, Length=394, Percent_Identity=26.1421319796954, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI56713256, Length=368, Percent_Identity=30.1630434782609, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI56713254, Length=368, Percent_Identity=30.1630434782609, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI169881281, Length=301, Percent_Identity=25.5813953488372, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4507369, Length=378, Percent_Identity=26.984126984127, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI187936925, Length=421, Percent_Identity=25.1781472684086, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI14211921, Length=421, Percent_Identity=25.1781472684086, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI215599424, Length=393, Percent_Identity=25.6997455470738, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19263340, Length=393, Percent_Identity=25.6997455470738, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI149944424, Length=240, Percent_Identity=30.4166666666667, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI4885351, Length=368, Percent_Identity=23.0978260869565, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786816, Length=361, Percent_Identity=27.9778393351801, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788722, Length=372, Percent_Identity=29.0322580645161, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788627, Length=366, Percent_Identity=32.2404371584699, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787909, Length=283, Percent_Identity=26.1484098939929, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790797, Length=344, Percent_Identity=23.8372093023256, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787710, Length=302, Percent_Identity=22.8476821192053, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788332, Length=172, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=379, Percent_Identity=31.3984168865435, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI71994476, Length=379, Percent_Identity=31.6622691292876, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17567369, Length=400, Percent_Identity=29.75, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17567663, Length=398, Percent_Identity=27.3869346733668, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71981209, Length=369, Percent_Identity=22.4932249322493, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322401, Length=300, Percent_Identity=28, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323118, Length=350, Percent_Identity=22.8571428571429, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320317, Length=350, Percent_Identity=22, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28573069, Length=410, Percent_Identity=28.0487804878049, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24646114, Length=410, Percent_Identity=28.0487804878049, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28573067, Length=410, Percent_Identity=28.0487804878049, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28573065, Length=410, Percent_Identity=28.0487804878049, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859735, Length=386, Percent_Identity=26.4248704663212, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24641770, Length=393, Percent_Identity=27.2264631043257, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641760, Length=393, Percent_Identity=27.2264631043257, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641768, Length=393, Percent_Identity=27.2264631043257, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641766, Length=393, Percent_Identity=27.2264631043257, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641764, Length=393, Percent_Identity=27.2264631043257, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45551451, Length=393, Percent_Identity=27.2264631043257, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUGH_BACSU (Q795M6)

Other databases:

- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   G70010
- RefSeq:   NP_391018.2
- HSSP:   Q56232
- ProteinModelPortal:   Q795M6
- SMR:   Q795M6
- EnsemblBacteria:   EBBACT00000001811
- GeneID:   938846
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31400
- GenoList:   BSU31400
- GeneTree:   EBGT00070000031834
- HOGENOM:   HBG645860
- OMA:   YTSNQGL
- PhylomeDB:   Q795M6
- ProtClustDB:   PRK07682
- BioCyc:   BSUB:BSU31400-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PRINTS:   PR00753

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.17

Molecular weight: Translated: 42311; Mature: 42180

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTS
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCC
YTANAGLYSLREEISRYLSNRFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPE
EECCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECC
PCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVTEKTKAILICSPSNPTGSVYS
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCC
KEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG
HHHHHHHHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHCC
WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESLNEIGLSCHHPGGAFYAFPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYI
HHHHHHCCCEEECCCCCEEECCCHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
RCSYATSIEQLQEALVRMKRFLHKTT
EEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTS
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCC
YTANAGLYSLREEISRYLSNRFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPE
EECCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECC
PCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVTEKTKAILICSPSNPTGSVYS
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCC
KEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG
HHHHHHHHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHCC
WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESLNEIGLSCHHPGGAFYAFPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYI
HHHHHHCCCEEECCCCCEEECCCHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
RCSYATSIEQLQEALVRMKRFLHKTT
EEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-succinyl-L-2,6-diaminoheptanedioate; 2-oxoglutarate

Specific reaction: N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377