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The map label for this gene is iscS

Identifier: 50812274

GI number: 50812274

Start: 2810559

End: 2811698

Strand: Reverse

Name: iscS

Synonym: BSU27510

Alternate gene names: 50812274

Gene position: 2811698-2810559 (Counterclockwise)

Preceding gene: 255767629

Following gene: 255767628

Centisome position: 66.7

GC content: 48.16

Gene sequence:

>1140_bases
ATGGAACGGATTTATTTAGATCATGCCGCAACGTCTCCGATGGATGAGCGCGTGCTGGAACAAATGATACCGCACTTCTC
CGGCAGTTTCGGTAATCCCTCCAGTATTCATTCATTTGGCAGAGAATCTCGAAAATGGGTGGATGAAGCAAGAGCGCAGA
TCGCAGCAGAAATCGGAGCGGCAGAGCAGGAGATCATTTTTACAAGCGGGGGAACGGAAGCTGATAACTTGGCGATCATG
GGAACCGCACTTGCAAGAAAAGATCTTGGCAGACATATCATCACAACAAAAATTGAGCATCACGCCGTGCTTCACACATG
TGAAAAACTTGAGGGAGACGGATTTGACATAACGTATCTGGACGTTGACCAAAATGGAAGAGTCAGTGCAAAACAGGTGA
AAGAAGCACTGCGTGATGATACAATCCTTGTGACAGTGATGTATGGAAATAATGAAGTCGGAACAGTGCAGCCGATCGAA
GAAATCGGTGAGCTGTTAAAGGAACACAAAGCTTATTTTCATACCGATGCTGTTCAGGCATTCGGGTTGCTGCCAATTGA
TGTGAAAAACAGCCATATTGACCTTCTGTCTGTTTCCGGGCACAAGCTCAACGGGCCAAAAGGGACAGGCTTTTTATATG
CTAGTAAAGATGTGAAGCTTTCACCCCTTTTATTCGGAGGAGAGCAAGAAAGAAAACGCCGTGCCGGAACGGAAAATGTT
CCAGGCATTGTCGGGCTGAAAGAAGCGATCAAGCTGTCAAGTGAAGAACGTGACGAAAAAAACGAGAAGTATCAATCGTT
TAAAGCAATCTTTGCCGACACGCTTAGAGATGCAGGTGTGGCATTCGAGGTCAACGGGGACAAAGAGCATAGTCTGCCGC
ATGTTCTGAATCTTTATTTCCCCGGTGTGTCAGTGGAAGCGCTGCTGGTTAATCTTGATATGGCTGGTGTCGCTGTCTCC
AGCGGTTCAGCGTGCACGGCCGGTTCAGTCCTGCCGTCACATGTTCTGACTGCTATGTTCGGCGAAGAGAGTGACCGGCT
GACGTCCTCTATTCGAATCAGCTTCGGCCTCGGCAATACGGCTGAGCAAGTGAAAACTGCTGCCAAACATGTGGCCGACG
TTGTCAAACGGCTGACATAA

Upstream 100 bases:

>100_bases
AGAGGTTTTAGACAGTACAACATTAGAGGATCTTGCCAGCTATACAGACGGAGAGCAAGAAGCTTATATGTTCTATATTT
AGATCAGAGGTGTAAACATA

Downstream 100 bases:

>100_bases
TAAGGCGGCGTAGTAATTGGAGTGAATGAAATGGAAAAACGGCCGGAGGATACAAGAGTCGTCGTCGGCATGTCCGGCGG
AGTAGACTCATCTGTGGCGG

Product: cysteine desulfurase involved in tRNA thiolation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIM
GTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYLDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIE
EIGELLKEHKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV
PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVS
SGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT

Sequences:

>Translated_379_residues
MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIM
GTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYLDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIE
EIGELLKEHKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV
PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVS
SGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT
>Mature_379_residues
MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIM
GTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYLDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIE
EIGELLKEHKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV
PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVS
SGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=42.257217847769, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=30.6954436450839, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI48994898, Length=380, Percent_Identity=44.2105263157895, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1789175, Length=383, Percent_Identity=25.8485639686684, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787970, Length=270, Percent_Identity=25.5555555555556, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=380, Percent_Identity=43.9473684210526, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=32.3076923076923, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=381, Percent_Identity=43.5695538057743, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI20129463, Length=378, Percent_Identity=43.9153439153439, Blast_Score=297, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS1_BACSU (O34599)

Other databases:

- EMBL:   AL009126
- PIR:   E69981
- RefSeq:   NP_390629.2
- HSSP:   P0A6B7
- ProteinModelPortal:   O34599
- SMR:   O34599
- EnsemblBacteria:   EBBACT00000003550
- GeneID:   937550
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27510
- GenoList:   BSU27510
- GeneTree:   EBGT00050000000894
- HOGENOM:   HBG635316
- OMA:   DEARRVC
- PhylomeDB:   O34599
- ProtClustDB:   CLSK928150
- BioCyc:   BSUB:BSU27510-MONOMER
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 41192; Mature: 41192

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGA
CCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYL
CCEEEEEECCCCCCCCEEEEHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCCCEEEEE
DVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQA
EECCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV
HCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCEECEEEECCHHHHHHHCCCCCC
PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEC
PGVSVEALLVNLDMAGVAVSSGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNT
CCCCEEEEEEECCHHCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEECCCCH
AEQVKTAAKHVADVVKRLT
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGA
CCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYL
CCEEEEEECCCCCCCCEEEEHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCCCEEEEE
DVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQA
EECCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENV
HCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCEECEEEECCHHHHHHHCCCCCC
PGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEC
PGVSVEALLVNLDMAGVAVSSGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNT
CCCCEEEEEEECCHHCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEECCCCH
AEQVKTAAKHVADVVKRLT
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377